Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35105 and RR42_RS35110 are separated by 88 nucleotides RR42_RS35110 and RR42_RS35115 are separated by 152 nucleotides RR42_RS35115 and RR42_RS35120 are separated by 52 nucleotides
RR42_RS35105: RR42_RS35105 - hydrolase, at 3,328,330 to 3,329,418
_RS35105
RR42_RS35110: RR42_RS35110 - hypothetical protein, at 3,329,507 to 3,329,689
_RS35110
RR42_RS35115: RR42_RS35115 - riboflavin synthase subunit beta, at 3,329,842 to 3,330,312
_RS35115
RR42_RS35120: RR42_RS35120 - benzoyl-CoA oxygenase, at 3,330,365 to 3,331,645
_RS35120
Position (kb)
3329
3330 Strain fitness (log2 ratio)
-1
0
1
2 at 3328.599 kb on - strand, within RR42_RS35105 at 3328.599 kb on - strand, within RR42_RS35105 at 3328.599 kb on - strand, within RR42_RS35105 at 3328.632 kb on + strand, within RR42_RS35105 at 3328.633 kb on - strand, within RR42_RS35105 at 3328.745 kb on - strand, within RR42_RS35105 at 3328.745 kb on - strand, within RR42_RS35105 at 3328.745 kb on - strand, within RR42_RS35105 at 3328.962 kb on + strand, within RR42_RS35105 at 3328.963 kb on - strand, within RR42_RS35105 at 3329.101 kb on + strand, within RR42_RS35105 at 3329.421 kb on + strand at 3329.515 kb on + strand at 3329.515 kb on + strand at 3329.515 kb on + strand at 3329.515 kb on + strand at 3329.516 kb on - strand at 3329.516 kb on - strand at 3329.516 kb on - strand at 3329.606 kb on + strand, within RR42_RS35110 at 3329.606 kb on + strand at 3329.607 kb on - strand, within RR42_RS35110 at 3329.689 kb on + strand at 3329.690 kb on - strand at 3330.094 kb on + strand, within RR42_RS35115 at 3330.094 kb on + strand, within RR42_RS35115 at 3330.095 kb on - strand, within RR42_RS35115 at 3330.370 kb on - strand at 3330.501 kb on + strand, within RR42_RS35120 at 3330.502 kb on - strand, within RR42_RS35120 at 3330.512 kb on - strand, within RR42_RS35120 at 3330.657 kb on + strand, within RR42_RS35120 at 3330.657 kb on + strand, within RR42_RS35120 at 3330.657 kb on + strand, within RR42_RS35120 at 3330.658 kb on - strand, within RR42_RS35120
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 3,328,599 - RR42_RS35105 0.25 +0.3 3,328,599 - RR42_RS35105 0.25 -0.0 3,328,599 - RR42_RS35105 0.25 +0.3 3,328,632 + RR42_RS35105 0.28 +0.8 3,328,633 - RR42_RS35105 0.28 +1.5 3,328,745 - RR42_RS35105 0.38 +0.5 3,328,745 - RR42_RS35105 0.38 -0.2 3,328,745 - RR42_RS35105 0.38 +0.3 3,328,962 + RR42_RS35105 0.58 +0.2 3,328,963 - RR42_RS35105 0.58 +0.6 3,329,101 + RR42_RS35105 0.71 +1.3 3,329,421 + -0.1 3,329,515 + +0.5 3,329,515 + -0.1 3,329,515 + -0.4 3,329,515 + +0.6 3,329,516 - -0.7 3,329,516 - +0.0 3,329,516 - +0.1 3,329,606 + RR42_RS35110 0.54 +0.3 3,329,606 + +0.1 3,329,607 - RR42_RS35110 0.55 +2.2 3,329,689 + -1.1 3,329,690 - -0.5 3,330,094 + RR42_RS35115 0.54 -0.5 3,330,094 + RR42_RS35115 0.54 -0.5 3,330,095 - RR42_RS35115 0.54 -0.2 3,330,370 - +1.1 3,330,501 + RR42_RS35120 0.11 -1.0 3,330,502 - RR42_RS35120 0.11 +0.1 3,330,512 - RR42_RS35120 0.11 +0.5 3,330,657 + RR42_RS35120 0.23 +0.0 3,330,657 + RR42_RS35120 0.23 -0.3 3,330,657 + RR42_RS35120 0.23 -1.5 3,330,658 - RR42_RS35120 0.23 +2.1
Or see this region's nucleotide sequence