Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34320 and RR42_RS34325 are separated by 79 nucleotides RR42_RS34325 and RR42_RS34330 are separated by 384 nucleotides
RR42_RS34320: RR42_RS34320 - IclR family transcriptional regulator, at 3,127,489 to 3,128,283
_RS34320
RR42_RS34325: RR42_RS34325 - hypothetical protein, at 3,128,363 to 3,128,671
_RS34325
RR42_RS34330: RR42_RS34330 - hypothetical protein, at 3,129,056 to 3,129,280
_RS34330
Position (kb)
3129
3130 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3128.292 kb on - strand at 3128.445 kb on + strand, within RR42_RS34325 at 3128.445 kb on + strand, within RR42_RS34325 at 3128.490 kb on + strand, within RR42_RS34325 at 3128.490 kb on + strand, within RR42_RS34325 at 3128.490 kb on + strand, within RR42_RS34325 at 3128.491 kb on - strand, within RR42_RS34325 at 3128.541 kb on + strand, within RR42_RS34325 at 3128.542 kb on - strand, within RR42_RS34325 at 3128.652 kb on + strand at 3128.652 kb on + strand at 3128.652 kb on + strand at 3128.653 kb on - strand at 3128.653 kb on - strand at 3128.742 kb on + strand at 3128.743 kb on - strand at 3128.782 kb on - strand at 3128.831 kb on + strand at 3128.831 kb on + strand at 3128.832 kb on - strand at 3128.832 kb on - strand at 3128.832 kb on - strand at 3128.832 kb on - strand at 3128.832 kb on - strand at 3128.832 kb on - strand at 3128.916 kb on + strand at 3128.916 kb on + strand at 3128.917 kb on - strand at 3128.917 kb on - strand at 3129.001 kb on + strand at 3129.001 kb on + strand at 3129.002 kb on - strand at 3129.002 kb on - strand at 3129.002 kb on - strand at 3129.002 kb on - strand at 3129.016 kb on + strand at 3129.016 kb on + strand at 3129.016 kb on + strand at 3129.017 kb on - strand at 3129.118 kb on + strand, within RR42_RS34330 at 3129.119 kb on - strand, within RR42_RS34330 at 3129.119 kb on - strand, within RR42_RS34330 at 3129.410 kb on + strand at 3129.411 kb on - strand at 3129.461 kb on + strand at 3129.461 kb on + strand at 3129.462 kb on - strand at 3129.462 kb on - strand at 3129.462 kb on - strand at 3129.487 kb on + strand at 3129.634 kb on - strand at 3129.687 kb on + strand at 3129.688 kb on - strand at 3129.688 kb on - strand at 3129.701 kb on - strand at 3129.759 kb on + strand at 3129.760 kb on - strand at 3129.760 kb on - strand at 3129.811 kb on - strand at 3129.911 kb on + strand at 3129.912 kb on - strand at 3129.955 kb on + strand at 3129.955 kb on + strand at 3129.955 kb on + strand at 3129.955 kb on + strand at 3129.994 kb on + strand at 3130.119 kb on + strand at 3130.120 kb on - strand at 3130.120 kb on - strand at 3130.120 kb on - strand at 3130.120 kb on - strand at 3130.174 kb on - strand at 3130.212 kb on + strand at 3130.213 kb on - strand at 3130.213 kb on - strand at 3130.213 kb on - strand at 3130.256 kb on + strand at 3130.256 kb on + strand at 3130.257 kb on - strand at 3130.257 kb on - strand at 3130.257 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 3,128,292 - -0.1 3,128,445 + RR42_RS34325 0.27 +0.1 3,128,445 + RR42_RS34325 0.27 -0.6 3,128,490 + RR42_RS34325 0.41 +1.1 3,128,490 + RR42_RS34325 0.41 -1.0 3,128,490 + RR42_RS34325 0.41 -0.5 3,128,491 - RR42_RS34325 0.41 -2.2 3,128,541 + RR42_RS34325 0.58 +0.4 3,128,542 - RR42_RS34325 0.58 -0.7 3,128,652 + -1.4 3,128,652 + -1.0 3,128,652 + -0.8 3,128,653 - +0.9 3,128,653 - +0.6 3,128,742 + +0.4 3,128,743 - +0.2 3,128,782 - -0.1 3,128,831 + -0.9 3,128,831 + -0.3 3,128,832 - +0.0 3,128,832 - -0.2 3,128,832 - +0.1 3,128,832 - -0.4 3,128,832 - -1.0 3,128,832 - +1.5 3,128,916 + -0.6 3,128,916 + -1.3 3,128,917 - -0.5 3,128,917 - +0.0 3,129,001 + +0.1 3,129,001 + +0.4 3,129,002 - -0.7 3,129,002 - +0.5 3,129,002 - -0.3 3,129,002 - +0.1 3,129,016 + -0.3 3,129,016 + -1.2 3,129,016 + +1.1 3,129,017 - -1.0 3,129,118 + RR42_RS34330 0.28 -0.2 3,129,119 - RR42_RS34330 0.28 +0.5 3,129,119 - RR42_RS34330 0.28 -0.5 3,129,410 + -2.2 3,129,411 - -1.9 3,129,461 + -0.2 3,129,461 + -0.1 3,129,462 - -0.6 3,129,462 - -0.4 3,129,462 - +0.2 3,129,487 + +0.1 3,129,634 - +0.6 3,129,687 + -1.7 3,129,688 - -0.2 3,129,688 - -0.1 3,129,701 - +0.5 3,129,759 + +0.1 3,129,760 - -0.1 3,129,760 - -1.8 3,129,811 - -0.3 3,129,911 + -0.5 3,129,912 - +0.0 3,129,955 + +0.3 3,129,955 + -1.0 3,129,955 + +0.3 3,129,955 + -0.8 3,129,994 + +0.8 3,130,119 + -1.0 3,130,120 - -0.7 3,130,120 - +2.7 3,130,120 - -0.2 3,130,120 - +0.1 3,130,174 - +0.8 3,130,212 + -0.4 3,130,213 - +1.7 3,130,213 - +0.1 3,130,213 - +0.1 3,130,256 + +0.6 3,130,256 + -0.2 3,130,257 - +0.9 3,130,257 - +0.5 3,130,257 - -0.7
Or see this region's nucleotide sequence