Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33780 and RR42_RS33785 are separated by 288 nucleotides RR42_RS33785 and RR42_RS33790 are separated by 37 nucleotides
RR42_RS33780: RR42_RS33780 - GABA permease, at 3,008,155 to 3,009,579
_RS33780
RR42_RS33785: RR42_RS33785 - transcriptional regulator, at 3,009,868 to 3,010,278
_RS33785
RR42_RS33790: RR42_RS33790 - FAD-dependent oxidoreductase, at 3,010,316 to 3,011,755
_RS33790
Position (kb)
3009
3010
3011 Strain fitness (log2 ratio)
-2
-1
0
1 at 3008.923 kb on + strand, within RR42_RS33780 at 3008.923 kb on + strand, within RR42_RS33780 at 3008.923 kb on + strand, within RR42_RS33780 at 3008.923 kb on + strand, within RR42_RS33780 at 3008.923 kb on + strand, within RR42_RS33780 at 3008.924 kb on - strand, within RR42_RS33780 at 3008.924 kb on - strand, within RR42_RS33780 at 3008.924 kb on - strand, within RR42_RS33780 at 3008.924 kb on - strand, within RR42_RS33780 at 3008.990 kb on - strand, within RR42_RS33780 at 3008.990 kb on - strand, within RR42_RS33780 at 3008.990 kb on - strand, within RR42_RS33780 at 3008.990 kb on - strand, within RR42_RS33780 at 3009.088 kb on + strand, within RR42_RS33780 at 3009.230 kb on - strand, within RR42_RS33780 at 3009.230 kb on - strand, within RR42_RS33780 at 3009.234 kb on - strand, within RR42_RS33780 at 3009.271 kb on + strand, within RR42_RS33780 at 3009.271 kb on + strand, within RR42_RS33780 at 3009.272 kb on - strand, within RR42_RS33780 at 3009.295 kb on + strand, within RR42_RS33780 at 3009.295 kb on + strand, within RR42_RS33780 at 3009.296 kb on - strand, within RR42_RS33780 at 3009.445 kb on - strand at 3009.508 kb on + strand at 3009.509 kb on - strand at 3009.763 kb on - strand at 3009.785 kb on + strand at 3009.785 kb on + strand at 3009.785 kb on + strand at 3009.786 kb on - strand at 3009.799 kb on - strand at 3010.433 kb on + strand at 3010.434 kb on - strand at 3010.445 kb on + strand at 3010.805 kb on + strand, within RR42_RS33790 at 3010.806 kb on - strand, within RR42_RS33790 at 3010.806 kb on - strand, within RR42_RS33790 at 3010.919 kb on + strand, within RR42_RS33790 at 3011.084 kb on + strand, within RR42_RS33790 at 3011.084 kb on + strand, within RR42_RS33790 at 3011.085 kb on - strand, within RR42_RS33790 at 3011.146 kb on + strand, within RR42_RS33790 at 3011.146 kb on + strand, within RR42_RS33790 at 3011.147 kb on - strand, within RR42_RS33790
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 3,008,923 + RR42_RS33780 0.54 -1.8 3,008,923 + RR42_RS33780 0.54 -0.4 3,008,923 + RR42_RS33780 0.54 -0.4 3,008,923 + RR42_RS33780 0.54 -2.0 3,008,923 + RR42_RS33780 0.54 -1.1 3,008,924 - RR42_RS33780 0.54 -0.2 3,008,924 - RR42_RS33780 0.54 +0.0 3,008,924 - RR42_RS33780 0.54 +0.5 3,008,924 - RR42_RS33780 0.54 +0.2 3,008,990 - RR42_RS33780 0.59 +1.1 3,008,990 - RR42_RS33780 0.59 +0.1 3,008,990 - RR42_RS33780 0.59 -1.1 3,008,990 - RR42_RS33780 0.59 +0.3 3,009,088 + RR42_RS33780 0.65 -0.8 3,009,230 - RR42_RS33780 0.75 -0.3 3,009,230 - RR42_RS33780 0.75 +0.2 3,009,234 - RR42_RS33780 0.76 -0.1 3,009,271 + RR42_RS33780 0.78 -0.1 3,009,271 + RR42_RS33780 0.78 -0.0 3,009,272 - RR42_RS33780 0.78 -0.9 3,009,295 + RR42_RS33780 0.80 -0.7 3,009,295 + RR42_RS33780 0.80 -0.4 3,009,296 - RR42_RS33780 0.80 +0.3 3,009,445 - -0.8 3,009,508 + -0.8 3,009,509 - -0.3 3,009,763 - +0.3 3,009,785 + -0.2 3,009,785 + -1.1 3,009,785 + -0.5 3,009,786 - -0.2 3,009,799 - +0.3 3,010,433 + +0.5 3,010,434 - -1.3 3,010,445 + -0.6 3,010,805 + RR42_RS33790 0.34 -1.0 3,010,806 - RR42_RS33790 0.34 -0.8 3,010,806 - RR42_RS33790 0.34 -0.5 3,010,919 + RR42_RS33790 0.42 -0.2 3,011,084 + RR42_RS33790 0.53 -0.2 3,011,084 + RR42_RS33790 0.53 -0.2 3,011,085 - RR42_RS33790 0.53 -0.5 3,011,146 + RR42_RS33790 0.58 +0.4 3,011,146 + RR42_RS33790 0.58 -2.2 3,011,147 - RR42_RS33790 0.58 -0.8
Or see this region's nucleotide sequence