Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33770 and RR42_RS33775 are separated by 181 nucleotides RR42_RS33775 and RR42_RS33780 are separated by 232 nucleotides
RR42_RS33770: RR42_RS33770 - cobalamin biosynthesis protein CobS, at 3,004,931 to 3,005,908
_RS33770
RR42_RS33775: RR42_RS33775 - sulfoacetaldehyde acetyltransferase, at 3,006,090 to 3,007,922
_RS33775
RR42_RS33780: RR42_RS33780 - GABA permease, at 3,008,155 to 3,009,579
_RS33780
Position (kb)
3006
3007
3008 Strain fitness (log2 ratio)
-2
-1
0
1 at 3005.265 kb on + strand, within RR42_RS33770 at 3005.265 kb on + strand, within RR42_RS33770 at 3005.265 kb on + strand, within RR42_RS33770 at 3005.265 kb on + strand, within RR42_RS33770 at 3005.265 kb on + strand, within RR42_RS33770 at 3005.266 kb on - strand, within RR42_RS33770 at 3005.266 kb on - strand, within RR42_RS33770 at 3005.331 kb on + strand, within RR42_RS33770 at 3005.332 kb on - strand, within RR42_RS33770 at 3005.620 kb on + strand, within RR42_RS33770 at 3005.750 kb on + strand, within RR42_RS33770 at 3005.750 kb on + strand, within RR42_RS33770 at 3005.948 kb on - strand at 3007.905 kb on + strand at 3007.905 kb on + strand at 3007.914 kb on + strand at 3008.098 kb on - strand at 3008.103 kb on + strand at 3008.103 kb on + strand at 3008.103 kb on + strand at 3008.103 kb on + strand at 3008.104 kb on - strand at 3008.104 kb on - strand at 3008.106 kb on + strand at 3008.106 kb on + strand at 3008.106 kb on + strand at 3008.107 kb on - strand at 3008.108 kb on + strand at 3008.108 kb on + strand at 3008.109 kb on - strand at 3008.109 kb on - strand at 3008.109 kb on - strand at 3008.109 kb on - strand at 3008.110 kb on + strand at 3008.110 kb on + strand at 3008.111 kb on - strand at 3008.111 kb on - strand at 3008.398 kb on + strand, within RR42_RS33780 at 3008.398 kb on + strand, within RR42_RS33780 at 3008.473 kb on + strand, within RR42_RS33780 at 3008.474 kb on - strand, within RR42_RS33780 at 3008.474 kb on - strand, within RR42_RS33780 at 3008.474 kb on - strand, within RR42_RS33780 at 3008.479 kb on + strand, within RR42_RS33780 at 3008.479 kb on + strand, within RR42_RS33780 at 3008.480 kb on - strand, within RR42_RS33780 at 3008.480 kb on - strand, within RR42_RS33780 at 3008.480 kb on - strand, within RR42_RS33780 at 3008.576 kb on + strand, within RR42_RS33780 at 3008.577 kb on - strand, within RR42_RS33780 at 3008.577 kb on - strand, within RR42_RS33780 at 3008.577 kb on - strand, within RR42_RS33780 at 3008.770 kb on - strand, within RR42_RS33780 at 3008.818 kb on + strand, within RR42_RS33780 at 3008.819 kb on - strand, within RR42_RS33780
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 3,005,265 + RR42_RS33770 0.34 +0.4 3,005,265 + RR42_RS33770 0.34 -1.0 3,005,265 + RR42_RS33770 0.34 -1.0 3,005,265 + RR42_RS33770 0.34 -0.1 3,005,265 + RR42_RS33770 0.34 +0.3 3,005,266 - RR42_RS33770 0.34 +0.0 3,005,266 - RR42_RS33770 0.34 -1.6 3,005,331 + RR42_RS33770 0.41 +0.3 3,005,332 - RR42_RS33770 0.41 -0.9 3,005,620 + RR42_RS33770 0.70 -0.0 3,005,750 + RR42_RS33770 0.84 +0.6 3,005,750 + RR42_RS33770 0.84 -0.7 3,005,948 - +0.3 3,007,905 + -0.0 3,007,905 + +0.4 3,007,914 + -1.0 3,008,098 - -0.2 3,008,103 + +1.4 3,008,103 + -0.5 3,008,103 + +0.5 3,008,103 + +0.7 3,008,104 - -0.5 3,008,104 - -0.8 3,008,106 + -2.1 3,008,106 + +0.8 3,008,106 + +0.0 3,008,107 - -1.1 3,008,108 + -0.7 3,008,108 + +0.5 3,008,109 - +0.4 3,008,109 - -0.6 3,008,109 - -1.4 3,008,109 - -1.6 3,008,110 + +0.4 3,008,110 + +0.3 3,008,111 - +1.4 3,008,111 - -0.2 3,008,398 + RR42_RS33780 0.17 -0.4 3,008,398 + RR42_RS33780 0.17 +0.6 3,008,473 + RR42_RS33780 0.22 -0.4 3,008,474 - RR42_RS33780 0.22 +0.9 3,008,474 - RR42_RS33780 0.22 -0.3 3,008,474 - RR42_RS33780 0.22 +1.0 3,008,479 + RR42_RS33780 0.23 +0.3 3,008,479 + RR42_RS33780 0.23 -1.6 3,008,480 - RR42_RS33780 0.23 -0.2 3,008,480 - RR42_RS33780 0.23 -0.5 3,008,480 - RR42_RS33780 0.23 -0.4 3,008,576 + RR42_RS33780 0.30 +0.1 3,008,577 - RR42_RS33780 0.30 -0.3 3,008,577 - RR42_RS33780 0.30 +0.4 3,008,577 - RR42_RS33780 0.30 +0.5 3,008,770 - RR42_RS33780 0.43 +0.2 3,008,818 + RR42_RS33780 0.47 +0.3 3,008,819 - RR42_RS33780 0.47 -0.7
Or see this region's nucleotide sequence