Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33585 and RR42_RS33590 are separated by 104 nucleotides RR42_RS33590 and RR42_RS33595 are separated by 11 nucleotides RR42_RS33595 and RR42_RS33600 overlap by 8 nucleotides RR42_RS33600 and RR42_RS33605 are separated by 135 nucleotides
RR42_RS33585: RR42_RS33585 - 3-keto-5-aminohexanoate cleavage protein, at 2,963,341 to 2,964,273
_RS33585
RR42_RS33590: RR42_RS33590 - cyclase, at 2,964,378 to 2,965,136
_RS33590
RR42_RS33595: RR42_RS33595 - hypothetical protein, at 2,965,148 to 2,965,522
_RS33595
RR42_RS33600: RR42_RS33600 - oxidoreductase, at 2,965,515 to 2,966,303
_RS33600
RR42_RS33605: RR42_RS33605 - ABC transporter substrate-binding protein, at 2,966,439 to 2,967,716
_RS33605
Position (kb)
2965
2966 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2964.286 kb on + strand at 2964.286 kb on + strand at 2964.331 kb on - strand at 2964.407 kb on + strand at 2964.485 kb on + strand, within RR42_RS33590 at 2964.506 kb on + strand, within RR42_RS33590 at 2964.655 kb on - strand, within RR42_RS33590 at 2964.730 kb on - strand, within RR42_RS33590 at 2964.733 kb on - strand, within RR42_RS33590 at 2964.739 kb on - strand, within RR42_RS33590 at 2964.753 kb on + strand, within RR42_RS33590 at 2964.753 kb on + strand, within RR42_RS33590 at 2964.754 kb on - strand, within RR42_RS33590 at 2964.754 kb on - strand, within RR42_RS33590 at 2964.892 kb on - strand, within RR42_RS33590 at 2964.892 kb on - strand, within RR42_RS33590 at 2965.078 kb on + strand at 2965.078 kb on + strand at 2965.078 kb on + strand at 2965.079 kb on - strand at 2965.325 kb on + strand, within RR42_RS33595 at 2965.326 kb on - strand, within RR42_RS33595 at 2965.326 kb on - strand, within RR42_RS33595 at 2965.498 kb on + strand at 2965.499 kb on - strand at 2965.499 kb on - strand at 2965.520 kb on + strand at 2965.520 kb on + strand at 2965.524 kb on - strand at 2965.555 kb on + strand at 2965.556 kb on - strand at 2965.556 kb on - strand at 2966.177 kb on + strand, within RR42_RS33600 at 2966.272 kb on + strand at 2966.273 kb on - strand at 2966.273 kb on - strand at 2966.274 kb on + strand at 2966.275 kb on - strand at 2966.275 kb on - strand at 2966.275 kb on - strand at 2966.290 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,964,286 + +0.7 2,964,286 + +0.7 2,964,331 - +1.8 2,964,407 + -0.1 2,964,485 + RR42_RS33590 0.14 -0.1 2,964,506 + RR42_RS33590 0.17 -0.3 2,964,655 - RR42_RS33590 0.36 +0.4 2,964,730 - RR42_RS33590 0.46 -0.3 2,964,733 - RR42_RS33590 0.47 +0.3 2,964,739 - RR42_RS33590 0.48 -0.2 2,964,753 + RR42_RS33590 0.49 -0.4 2,964,753 + RR42_RS33590 0.49 +0.1 2,964,754 - RR42_RS33590 0.50 -0.5 2,964,754 - RR42_RS33590 0.50 +1.1 2,964,892 - RR42_RS33590 0.68 +0.1 2,964,892 - RR42_RS33590 0.68 +0.2 2,965,078 + -0.7 2,965,078 + +1.2 2,965,078 + +1.0 2,965,079 - +0.3 2,965,325 + RR42_RS33595 0.47 -0.3 2,965,326 - RR42_RS33595 0.47 +0.1 2,965,326 - RR42_RS33595 0.47 -1.8 2,965,498 + -1.6 2,965,499 - +0.2 2,965,499 - -0.2 2,965,520 + -0.1 2,965,520 + -1.2 2,965,524 - -0.6 2,965,555 + +0.7 2,965,556 - -0.7 2,965,556 - +0.2 2,966,177 + RR42_RS33600 0.84 +0.3 2,966,272 + +0.2 2,966,273 - +0.7 2,966,273 - +0.5 2,966,274 + +0.6 2,966,275 - +0.6 2,966,275 - +0.8 2,966,275 - -0.0 2,966,290 - +0.1
Or see this region's nucleotide sequence