Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32380 and RR42_RS32385 overlap by 4 nucleotides RR42_RS32385 and RR42_RS32390 are separated by 62 nucleotides
RR42_RS32380: RR42_RS32380 - CoA-transferase, at 2,659,418 to 2,660,617
_RS32380
RR42_RS32385: RR42_RS32385 - citrate synthase, at 2,660,614 to 2,661,480
_RS32385
RR42_RS32390: RR42_RS32390 - citrate synthase, at 2,661,543 to 2,662,772
_RS32390
Position (kb)
2660
2661
2662 Strain fitness (log2 ratio)
-2
-1
0
1 at 2659.787 kb on + strand, within RR42_RS32380 at 2659.787 kb on + strand, within RR42_RS32380 at 2659.787 kb on + strand, within RR42_RS32380 at 2659.788 kb on - strand, within RR42_RS32380 at 2659.788 kb on - strand, within RR42_RS32380 at 2659.908 kb on - strand, within RR42_RS32380 at 2660.004 kb on - strand, within RR42_RS32380 at 2660.067 kb on - strand, within RR42_RS32380 at 2660.067 kb on - strand, within RR42_RS32380 at 2660.323 kb on + strand, within RR42_RS32380 at 2660.323 kb on + strand, within RR42_RS32380 at 2660.323 kb on + strand, within RR42_RS32380 at 2660.324 kb on - strand, within RR42_RS32380 at 2660.372 kb on + strand, within RR42_RS32380 at 2660.373 kb on - strand, within RR42_RS32380 at 2660.614 kb on - strand at 2660.625 kb on + strand at 2661.001 kb on + strand, within RR42_RS32385 at 2661.002 kb on - strand, within RR42_RS32385 at 2661.002 kb on - strand, within RR42_RS32385 at 2661.074 kb on - strand, within RR42_RS32385 at 2661.293 kb on - strand, within RR42_RS32385 at 2661.424 kb on + strand at 2661.424 kb on + strand at 2661.424 kb on + strand at 2661.424 kb on + strand at 2661.425 kb on - strand at 2661.425 kb on - strand at 2661.425 kb on - strand at 2661.425 kb on - strand at 2661.436 kb on + strand at 2661.655 kb on + strand at 2661.656 kb on - strand at 2661.656 kb on - strand at 2661.874 kb on + strand, within RR42_RS32390 at 2662.225 kb on + strand, within RR42_RS32390 at 2662.225 kb on + strand, within RR42_RS32390 at 2662.226 kb on - strand, within RR42_RS32390 at 2662.226 kb on - strand, within RR42_RS32390 at 2662.345 kb on + strand, within RR42_RS32390 at 2662.345 kb on + strand, within RR42_RS32390 at 2662.345 kb on + strand, within RR42_RS32390 at 2662.345 kb on + strand, within RR42_RS32390 at 2662.345 kb on + strand, within RR42_RS32390
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,659,787 + RR42_RS32380 0.31 -0.2 2,659,787 + RR42_RS32380 0.31 -0.2 2,659,787 + RR42_RS32380 0.31 +0.5 2,659,788 - RR42_RS32380 0.31 -0.7 2,659,788 - RR42_RS32380 0.31 -1.0 2,659,908 - RR42_RS32380 0.41 +0.1 2,660,004 - RR42_RS32380 0.49 -0.1 2,660,067 - RR42_RS32380 0.54 -0.5 2,660,067 - RR42_RS32380 0.54 -0.2 2,660,323 + RR42_RS32380 0.75 -0.4 2,660,323 + RR42_RS32380 0.75 -0.1 2,660,323 + RR42_RS32380 0.75 -0.5 2,660,324 - RR42_RS32380 0.76 -1.0 2,660,372 + RR42_RS32380 0.80 -0.0 2,660,373 - RR42_RS32380 0.80 +0.5 2,660,614 - +0.6 2,660,625 + -1.2 2,661,001 + RR42_RS32385 0.45 -0.7 2,661,002 - RR42_RS32385 0.45 -2.2 2,661,002 - RR42_RS32385 0.45 -0.4 2,661,074 - RR42_RS32385 0.53 -0.6 2,661,293 - RR42_RS32385 0.78 -0.2 2,661,424 + -0.1 2,661,424 + -0.2 2,661,424 + +0.1 2,661,424 + -0.3 2,661,425 - +0.4 2,661,425 - +0.1 2,661,425 - -1.8 2,661,425 - -0.2 2,661,436 + -0.6 2,661,655 + +0.3 2,661,656 - +0.1 2,661,656 - -0.2 2,661,874 + RR42_RS32390 0.27 -0.9 2,662,225 + RR42_RS32390 0.55 -1.1 2,662,225 + RR42_RS32390 0.55 -0.1 2,662,226 - RR42_RS32390 0.56 -0.3 2,662,226 - RR42_RS32390 0.56 -0.6 2,662,345 + RR42_RS32390 0.65 +0.4 2,662,345 + RR42_RS32390 0.65 -1.0 2,662,345 + RR42_RS32390 0.65 -1.5 2,662,345 + RR42_RS32390 0.65 +0.2 2,662,345 + RR42_RS32390 0.65 +1.0
Or see this region's nucleotide sequence