Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32050 and RR42_RS32055 are separated by 128 nucleotides RR42_RS32055 and RR42_RS32060 are separated by 137 nucleotides RR42_RS32060 and RR42_RS32065 are separated by 3 nucleotides
RR42_RS32050: RR42_RS32050 - AraC family transcriptional regulator, at 2,567,432 to 2,568,625
_RS32050
RR42_RS32055: RR42_RS32055 - 3-carboxymuconate cycloisomerase PcaB (EC 5.5.1.2) (from data) , at 2,568,754 to 2,570,079
_RS32055
RR42_RS32060: RR42_RS32060 - protocatechuate 3,4-dioxygenase, at 2,570,217 to 2,570,795
_RS32060
RR42_RS32065: RR42_RS32065 - protocatechuate 3,4-dioxygenase, beta subunit (EC 1.13.11.3) (from data) , at 2,570,799 to 2,571,524
_RS32065
Position (kb)
2568
2569
2570
2571 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2568.084 kb on + strand, within RR42_RS32050 at 2568.589 kb on - strand at 2568.589 kb on - strand at 2568.589 kb on - strand at 2568.660 kb on + strand at 2568.661 kb on - strand at 2568.661 kb on - strand at 2568.665 kb on + strand at 2568.804 kb on - strand at 2568.804 kb on - strand at 2569.128 kb on + strand, within RR42_RS32055 at 2569.129 kb on - strand, within RR42_RS32055 at 2569.397 kb on - strand, within RR42_RS32055 at 2569.407 kb on + strand, within RR42_RS32055 at 2569.408 kb on - strand, within RR42_RS32055 at 2569.408 kb on - strand, within RR42_RS32055 at 2570.377 kb on + strand, within RR42_RS32060 at 2570.377 kb on + strand, within RR42_RS32060 at 2570.377 kb on + strand, within RR42_RS32060 at 2570.377 kb on + strand, within RR42_RS32060 at 2570.377 kb on + strand, within RR42_RS32060 at 2570.378 kb on - strand, within RR42_RS32060 at 2570.380 kb on - strand, within RR42_RS32060 at 2570.380 kb on - strand, within RR42_RS32060 at 2570.415 kb on - strand, within RR42_RS32060 at 2570.415 kb on - strand, within RR42_RS32060 at 2570.432 kb on + strand, within RR42_RS32060 at 2570.432 kb on + strand, within RR42_RS32060 at 2570.432 kb on + strand, within RR42_RS32060 at 2570.759 kb on - strand at 2570.789 kb on - strand at 2570.800 kb on + strand at 2570.800 kb on + strand at 2570.801 kb on - strand at 2570.837 kb on + strand at 2570.974 kb on + strand, within RR42_RS32065 at 2570.974 kb on + strand, within RR42_RS32065 at 2570.974 kb on + strand, within RR42_RS32065 at 2570.974 kb on + strand, within RR42_RS32065 at 2570.975 kb on - strand, within RR42_RS32065 at 2570.975 kb on - strand, within RR42_RS32065 at 2570.975 kb on - strand, within RR42_RS32065 at 2570.975 kb on - strand, within RR42_RS32065 at 2571.001 kb on + strand, within RR42_RS32065 at 2571.002 kb on - strand, within RR42_RS32065 at 2571.029 kb on + strand, within RR42_RS32065 at 2571.030 kb on - strand, within RR42_RS32065 at 2571.030 kb on - strand, within RR42_RS32065 at 2571.030 kb on - strand, within RR42_RS32065 at 2571.030 kb on - strand, within RR42_RS32065 at 2571.030 kb on - strand, within RR42_RS32065
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,568,084 + RR42_RS32050 0.55 -0.3 2,568,589 - +0.7 2,568,589 - +0.5 2,568,589 - +0.8 2,568,660 + -0.7 2,568,661 - -0.1 2,568,661 - +0.2 2,568,665 + -0.2 2,568,804 - -0.3 2,568,804 - +0.2 2,569,128 + RR42_RS32055 0.28 +0.7 2,569,129 - RR42_RS32055 0.28 +0.0 2,569,397 - RR42_RS32055 0.48 +0.4 2,569,407 + RR42_RS32055 0.49 -0.4 2,569,408 - RR42_RS32055 0.49 +1.9 2,569,408 - RR42_RS32055 0.49 +0.4 2,570,377 + RR42_RS32060 0.28 +0.1 2,570,377 + RR42_RS32060 0.28 -0.2 2,570,377 + RR42_RS32060 0.28 -0.2 2,570,377 + RR42_RS32060 0.28 +0.1 2,570,377 + RR42_RS32060 0.28 -0.5 2,570,378 - RR42_RS32060 0.28 +0.4 2,570,380 - RR42_RS32060 0.28 -0.6 2,570,380 - RR42_RS32060 0.28 -1.4 2,570,415 - RR42_RS32060 0.34 -0.6 2,570,415 - RR42_RS32060 0.34 -0.6 2,570,432 + RR42_RS32060 0.37 -1.7 2,570,432 + RR42_RS32060 0.37 -0.8 2,570,432 + RR42_RS32060 0.37 +0.3 2,570,759 - -0.0 2,570,789 - -0.4 2,570,800 + -0.4 2,570,800 + -1.9 2,570,801 - -1.6 2,570,837 + -0.2 2,570,974 + RR42_RS32065 0.24 -0.4 2,570,974 + RR42_RS32065 0.24 +0.1 2,570,974 + RR42_RS32065 0.24 -0.9 2,570,974 + RR42_RS32065 0.24 +0.1 2,570,975 - RR42_RS32065 0.24 -0.2 2,570,975 - RR42_RS32065 0.24 +0.0 2,570,975 - RR42_RS32065 0.24 -0.9 2,570,975 - RR42_RS32065 0.24 +0.2 2,571,001 + RR42_RS32065 0.28 -0.6 2,571,002 - RR42_RS32065 0.28 +0.6 2,571,029 + RR42_RS32065 0.32 -1.0 2,571,030 - RR42_RS32065 0.32 +0.0 2,571,030 - RR42_RS32065 0.32 -1.1 2,571,030 - RR42_RS32065 0.32 +1.1 2,571,030 - RR42_RS32065 0.32 +0.4 2,571,030 - RR42_RS32065 0.32 +0.7
Or see this region's nucleotide sequence