Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31520 and RR42_RS31525 are separated by 125 nucleotides RR42_RS31525 and RR42_RS31530 overlap by 20 nucleotides RR42_RS31530 and RR42_RS31535 overlap by 1 nucleotides RR42_RS31535 and RR42_RS31540 are separated by 218 nucleotides
RR42_RS31520: RR42_RS31520 - 2-nitropropane dioxygenase, at 2,449,716 to 2,450,834
_RS31520
RR42_RS31525: RR42_RS31525 - threonine transporter, at 2,450,960 to 2,451,601
_RS31525
RR42_RS31530: RR42_RS31530 - histidine kinase, at 2,451,582 to 2,453,042
_RS31530
RR42_RS31535: RR42_RS31535 - transcriptional regulator, at 2,453,042 to 2,453,716
_RS31535
RR42_RS31540: RR42_RS31540 - RND transporter, at 2,453,935 to 2,457,180
_RS31540
Position (kb)
2451
2452
2453
2454 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2450.646 kb on + strand, within RR42_RS31520 at 2450.647 kb on - strand, within RR42_RS31520 at 2450.647 kb on - strand, within RR42_RS31520 at 2450.700 kb on + strand, within RR42_RS31520 at 2450.875 kb on + strand at 2450.875 kb on + strand at 2450.876 kb on - strand at 2450.876 kb on - strand at 2450.888 kb on + strand at 2450.888 kb on + strand at 2450.889 kb on - strand at 2450.889 kb on - strand at 2450.889 kb on - strand at 2451.083 kb on + strand, within RR42_RS31525 at 2451.083 kb on + strand, within RR42_RS31525 at 2451.084 kb on - strand, within RR42_RS31525 at 2451.084 kb on - strand, within RR42_RS31525 at 2451.176 kb on + strand, within RR42_RS31525 at 2451.176 kb on + strand, within RR42_RS31525 at 2451.176 kb on + strand, within RR42_RS31525 at 2451.176 kb on + strand, within RR42_RS31525 at 2451.177 kb on - strand, within RR42_RS31525 at 2451.206 kb on + strand, within RR42_RS31525 at 2451.206 kb on + strand, within RR42_RS31525 at 2451.207 kb on - strand, within RR42_RS31525 at 2451.207 kb on - strand, within RR42_RS31525 at 2451.215 kb on + strand, within RR42_RS31525 at 2451.216 kb on - strand, within RR42_RS31525 at 2451.216 kb on - strand, within RR42_RS31525 at 2451.369 kb on - strand, within RR42_RS31525 at 2451.513 kb on - strand, within RR42_RS31525 at 2451.555 kb on + strand at 2451.556 kb on - strand at 2451.599 kb on + strand at 2451.599 kb on + strand at 2452.475 kb on - strand, within RR42_RS31530 at 2452.619 kb on + strand, within RR42_RS31530 at 2452.960 kb on + strand at 2452.961 kb on - strand at 2453.583 kb on + strand, within RR42_RS31535 at 2453.584 kb on - strand, within RR42_RS31535 at 2453.584 kb on - strand, within RR42_RS31535 at 2453.584 kb on - strand, within RR42_RS31535 at 2453.674 kb on - strand at 2453.811 kb on + strand at 2453.853 kb on + strand at 2453.853 kb on + strand at 2453.854 kb on - strand at 2453.854 kb on - strand at 2453.968 kb on + strand at 2454.039 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,450,646 + RR42_RS31520 0.83 -0.6 2,450,647 - RR42_RS31520 0.83 -0.4 2,450,647 - RR42_RS31520 0.83 -0.1 2,450,700 + RR42_RS31520 0.88 -1.9 2,450,875 + +0.8 2,450,875 + +0.1 2,450,876 - -0.2 2,450,876 - -0.5 2,450,888 + -0.2 2,450,888 + -0.6 2,450,889 - +0.1 2,450,889 - +0.2 2,450,889 - -0.3 2,451,083 + RR42_RS31525 0.19 -0.9 2,451,083 + RR42_RS31525 0.19 +0.1 2,451,084 - RR42_RS31525 0.19 +0.2 2,451,084 - RR42_RS31525 0.19 -0.2 2,451,176 + RR42_RS31525 0.34 +0.6 2,451,176 + RR42_RS31525 0.34 -0.5 2,451,176 + RR42_RS31525 0.34 +0.6 2,451,176 + RR42_RS31525 0.34 +0.2 2,451,177 - RR42_RS31525 0.34 +1.8 2,451,206 + RR42_RS31525 0.38 -0.7 2,451,206 + RR42_RS31525 0.38 +0.9 2,451,207 - RR42_RS31525 0.38 -0.2 2,451,207 - RR42_RS31525 0.38 -0.6 2,451,215 + RR42_RS31525 0.40 -0.1 2,451,216 - RR42_RS31525 0.40 +0.6 2,451,216 - RR42_RS31525 0.40 +0.2 2,451,369 - RR42_RS31525 0.64 +0.6 2,451,513 - RR42_RS31525 0.86 +0.6 2,451,555 + -0.1 2,451,556 - -0.5 2,451,599 + -1.7 2,451,599 + -0.1 2,452,475 - RR42_RS31530 0.61 -1.7 2,452,619 + RR42_RS31530 0.71 -2.3 2,452,960 + +0.1 2,452,961 - -1.0 2,453,583 + RR42_RS31535 0.80 +0.6 2,453,584 - RR42_RS31535 0.80 -1.8 2,453,584 - RR42_RS31535 0.80 +0.0 2,453,584 - RR42_RS31535 0.80 -0.8 2,453,674 - -0.7 2,453,811 + -0.2 2,453,853 + +0.0 2,453,853 + -3.1 2,453,854 - -0.3 2,453,854 - +0.5 2,453,968 + -0.4 2,454,039 + +0.7
Or see this region's nucleotide sequence