Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31315 and RR42_RS31320 are separated by 208 nucleotides RR42_RS31320 and RR42_RS31325 are separated by 658 nucleotides
RR42_RS31315: RR42_RS31315 - NADH-quinone oxidoreductase subunit F, at 2,400,177 to 2,401,886
_RS31315
RR42_RS31320: RR42_RS31320 - spermidine/putrescine ABC transporter substrate-binding protein, at 2,402,095 to 2,403,411
_RS31320
RR42_RS31325: RR42_RS31325 - oxalate:formate antiporter, at 2,404,070 to 2,404,291
_RS31325
Position (kb)
2402
2403
2404 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2401.106 kb on + strand, within RR42_RS31315 at 2401.292 kb on - strand, within RR42_RS31315 at 2401.292 kb on - strand, within RR42_RS31315 at 2401.605 kb on + strand, within RR42_RS31315 at 2401.605 kb on + strand, within RR42_RS31315 at 2401.606 kb on - strand, within RR42_RS31315 at 2401.621 kb on + strand, within RR42_RS31315 at 2401.639 kb on + strand, within RR42_RS31315 at 2401.640 kb on - strand, within RR42_RS31315 at 2401.640 kb on - strand, within RR42_RS31315 at 2401.941 kb on + strand at 2401.941 kb on + strand at 2402.043 kb on + strand at 2402.043 kb on + strand at 2402.043 kb on + strand at 2402.156 kb on + strand at 2402.156 kb on + strand at 2402.157 kb on - strand at 2402.157 kb on - strand at 2402.157 kb on - strand at 2402.157 kb on - strand at 2402.325 kb on - strand, within RR42_RS31320 at 2402.396 kb on + strand, within RR42_RS31320 at 2402.396 kb on + strand, within RR42_RS31320 at 2402.396 kb on + strand, within RR42_RS31320 at 2402.396 kb on + strand, within RR42_RS31320 at 2402.396 kb on + strand, within RR42_RS31320 at 2402.396 kb on + strand, within RR42_RS31320 at 2402.396 kb on + strand, within RR42_RS31320 at 2402.397 kb on - strand, within RR42_RS31320 at 2402.397 kb on - strand, within RR42_RS31320 at 2402.397 kb on - strand, within RR42_RS31320 at 2402.397 kb on - strand, within RR42_RS31320 at 2402.462 kb on + strand, within RR42_RS31320 at 2402.467 kb on - strand, within RR42_RS31320 at 2402.467 kb on - strand, within RR42_RS31320 at 2402.730 kb on - strand, within RR42_RS31320 at 2402.741 kb on + strand, within RR42_RS31320 at 2402.780 kb on + strand, within RR42_RS31320 at 2402.780 kb on + strand, within RR42_RS31320 at 2402.780 kb on + strand, within RR42_RS31320 at 2402.780 kb on + strand, within RR42_RS31320 at 2402.781 kb on - strand, within RR42_RS31320 at 2402.781 kb on - strand, within RR42_RS31320 at 2402.837 kb on + strand, within RR42_RS31320 at 2402.837 kb on + strand, within RR42_RS31320 at 2402.837 kb on + strand, within RR42_RS31320 at 2403.044 kb on + strand, within RR42_RS31320 at 2403.045 kb on - strand, within RR42_RS31320 at 2403.176 kb on + strand, within RR42_RS31320 at 2403.176 kb on + strand, within RR42_RS31320 at 2403.176 kb on + strand, within RR42_RS31320 at 2403.177 kb on - strand, within RR42_RS31320 at 2403.177 kb on - strand, within RR42_RS31320 at 2403.177 kb on - strand, within RR42_RS31320 at 2403.222 kb on + strand, within RR42_RS31320 at 2403.272 kb on + strand, within RR42_RS31320 at 2403.273 kb on - strand, within RR42_RS31320 at 2403.273 kb on - strand, within RR42_RS31320 at 2403.311 kb on + strand at 2403.312 kb on - strand at 2403.352 kb on + strand at 2403.352 kb on + strand at 2403.352 kb on + strand at 2403.353 kb on - strand at 2403.442 kb on + strand at 2403.442 kb on + strand at 2403.443 kb on - strand at 2403.505 kb on + strand at 2403.506 kb on - strand at 2403.525 kb on + strand at 2403.564 kb on + strand at 2403.712 kb on - strand at 2403.849 kb on + strand at 2403.850 kb on - strand at 2403.944 kb on - strand at 2403.944 kb on - strand at 2403.944 kb on - strand at 2403.944 kb on - strand at 2403.944 kb on - strand at 2403.944 kb on - strand at 2404.216 kb on + strand, within RR42_RS31325 at 2404.332 kb on - strand at 2404.333 kb on + strand at 2404.334 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,401,106 + RR42_RS31315 0.54 +0.0 2,401,292 - RR42_RS31315 0.65 +0.8 2,401,292 - RR42_RS31315 0.65 +0.6 2,401,605 + RR42_RS31315 0.84 +0.2 2,401,605 + RR42_RS31315 0.84 -0.6 2,401,606 - RR42_RS31315 0.84 -0.2 2,401,621 + RR42_RS31315 0.84 -0.1 2,401,639 + RR42_RS31315 0.85 -0.3 2,401,640 - RR42_RS31315 0.86 -0.1 2,401,640 - RR42_RS31315 0.86 -0.6 2,401,941 + -0.3 2,401,941 + +0.9 2,402,043 + +0.9 2,402,043 + -0.6 2,402,043 + -0.2 2,402,156 + +0.7 2,402,156 + -0.6 2,402,157 - -0.5 2,402,157 - -1.0 2,402,157 - -0.5 2,402,157 - -0.8 2,402,325 - RR42_RS31320 0.17 +0.1 2,402,396 + RR42_RS31320 0.23 +1.6 2,402,396 + RR42_RS31320 0.23 -0.1 2,402,396 + RR42_RS31320 0.23 +2.7 2,402,396 + RR42_RS31320 0.23 +1.0 2,402,396 + RR42_RS31320 0.23 -0.1 2,402,396 + RR42_RS31320 0.23 +0.4 2,402,396 + RR42_RS31320 0.23 +0.4 2,402,397 - RR42_RS31320 0.23 +1.0 2,402,397 - RR42_RS31320 0.23 +0.7 2,402,397 - RR42_RS31320 0.23 +0.8 2,402,397 - RR42_RS31320 0.23 -0.3 2,402,462 + RR42_RS31320 0.28 -0.2 2,402,467 - RR42_RS31320 0.28 +0.1 2,402,467 - RR42_RS31320 0.28 +1.0 2,402,730 - RR42_RS31320 0.48 -0.3 2,402,741 + RR42_RS31320 0.49 +0.0 2,402,780 + RR42_RS31320 0.52 -1.2 2,402,780 + RR42_RS31320 0.52 +0.0 2,402,780 + RR42_RS31320 0.52 +1.0 2,402,780 + RR42_RS31320 0.52 -0.1 2,402,781 - RR42_RS31320 0.52 +0.2 2,402,781 - RR42_RS31320 0.52 +0.6 2,402,837 + RR42_RS31320 0.56 +1.1 2,402,837 + RR42_RS31320 0.56 +0.5 2,402,837 + RR42_RS31320 0.56 -0.1 2,403,044 + RR42_RS31320 0.72 -1.5 2,403,045 - RR42_RS31320 0.72 -0.6 2,403,176 + RR42_RS31320 0.82 -1.2 2,403,176 + RR42_RS31320 0.82 +1.0 2,403,176 + RR42_RS31320 0.82 -0.4 2,403,177 - RR42_RS31320 0.82 +1.1 2,403,177 - RR42_RS31320 0.82 +0.0 2,403,177 - RR42_RS31320 0.82 -0.3 2,403,222 + RR42_RS31320 0.86 +1.6 2,403,272 + RR42_RS31320 0.89 -0.4 2,403,273 - RR42_RS31320 0.89 +1.5 2,403,273 - RR42_RS31320 0.89 -0.3 2,403,311 + -0.5 2,403,312 - -1.9 2,403,352 + -0.5 2,403,352 + -0.0 2,403,352 + -1.2 2,403,353 - -0.9 2,403,442 + -0.8 2,403,442 + -2.3 2,403,443 - +0.8 2,403,505 + +0.2 2,403,506 - -1.5 2,403,525 + +0.5 2,403,564 + -0.2 2,403,712 - +0.8 2,403,849 + +0.9 2,403,850 - +0.0 2,403,944 - -0.1 2,403,944 - +0.6 2,403,944 - +0.7 2,403,944 - -1.2 2,403,944 - -3.5 2,403,944 - +0.1 2,404,216 + RR42_RS31325 0.66 +1.2 2,404,332 - -0.8 2,404,333 + -0.3 2,404,334 - +0.5
Or see this region's nucleotide sequence