Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30955 and RR42_RS30960 are separated by 28 nucleotides RR42_RS30960 and RR42_RS30965 are separated by 156 nucleotides RR42_RS30965 and RR42_RS30970 are separated by 87 nucleotides RR42_RS30970 and RR42_RS30975 are separated by 29 nucleotides
RR42_RS30955: RR42_RS30955 - histidine kinase, at 2,329,278 to 2,330,720
_RS30955
RR42_RS30960: RR42_RS30960 - transcriptional regulator, at 2,330,749 to 2,331,423
_RS30960
RR42_RS30965: RR42_RS30965 - hypothetical protein, at 2,331,580 to 2,332,125
_RS30965
RR42_RS30970: RR42_RS30970 - hypothetical protein, at 2,332,213 to 2,332,866
_RS30970
RR42_RS30975: RR42_RS30975 - blue copper protein, at 2,332,896 to 2,333,405
_RS30975
Position (kb)
2331
2332
2333 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2330.623 kb on + strand at 2330.624 kb on - strand at 2330.624 kb on - strand at 2330.710 kb on - strand at 2330.710 kb on - strand at 2330.775 kb on - strand at 2330.868 kb on + strand, within RR42_RS30960 at 2330.868 kb on + strand, within RR42_RS30960 at 2330.868 kb on + strand, within RR42_RS30960 at 2330.868 kb on + strand, within RR42_RS30960 at 2330.868 kb on + strand, within RR42_RS30960 at 2330.868 kb on + strand, within RR42_RS30960 at 2330.869 kb on - strand, within RR42_RS30960 at 2331.134 kb on + strand, within RR42_RS30960 at 2331.134 kb on + strand, within RR42_RS30960 at 2331.135 kb on - strand, within RR42_RS30960 at 2331.291 kb on - strand, within RR42_RS30960 at 2331.291 kb on - strand, within RR42_RS30960 at 2331.291 kb on - strand, within RR42_RS30960 at 2331.291 kb on - strand, within RR42_RS30960 at 2331.291 kb on - strand, within RR42_RS30960 at 2331.291 kb on - strand, within RR42_RS30960 at 2331.380 kb on + strand at 2331.380 kb on + strand at 2331.381 kb on - strand at 2331.381 kb on - strand at 2331.415 kb on + strand at 2331.415 kb on + strand at 2331.415 kb on + strand at 2331.416 kb on - strand at 2331.423 kb on + strand at 2331.423 kb on + strand at 2331.424 kb on - strand at 2331.424 kb on - strand at 2331.428 kb on - strand at 2331.428 kb on - strand at 2331.471 kb on - strand at 2331.471 kb on - strand at 2331.471 kb on - strand at 2331.471 kb on - strand at 2331.472 kb on + strand at 2331.473 kb on - strand at 2331.473 kb on - strand at 2331.549 kb on + strand at 2331.550 kb on - strand at 2331.554 kb on + strand at 2331.594 kb on + strand at 2331.595 kb on - strand at 2331.595 kb on - strand at 2331.598 kb on + strand at 2331.598 kb on + strand at 2331.599 kb on - strand at 2331.599 kb on - strand at 2331.757 kb on - strand, within RR42_RS30965 at 2331.761 kb on + strand, within RR42_RS30965 at 2331.762 kb on - strand, within RR42_RS30965 at 2331.762 kb on - strand, within RR42_RS30965 at 2331.762 kb on - strand, within RR42_RS30965 at 2332.357 kb on + strand, within RR42_RS30970 at 2332.357 kb on + strand, within RR42_RS30970 at 2332.357 kb on + strand, within RR42_RS30970 at 2332.361 kb on + strand, within RR42_RS30970 at 2332.621 kb on + strand, within RR42_RS30970 at 2332.621 kb on + strand, within RR42_RS30970 at 2332.622 kb on - strand, within RR42_RS30970 at 2332.622 kb on - strand, within RR42_RS30970 at 2332.622 kb on - strand, within RR42_RS30970 at 2332.667 kb on - strand, within RR42_RS30970 at 2332.838 kb on - strand at 2332.838 kb on - strand at 2332.838 kb on - strand at 2333.123 kb on + strand, within RR42_RS30975
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,330,623 + -0.3 2,330,624 - -1.2 2,330,624 - -1.0 2,330,710 - -1.2 2,330,710 - -2.0 2,330,775 - -1.3 2,330,868 + RR42_RS30960 0.18 +0.2 2,330,868 + RR42_RS30960 0.18 -0.2 2,330,868 + RR42_RS30960 0.18 +0.0 2,330,868 + RR42_RS30960 0.18 +0.3 2,330,868 + RR42_RS30960 0.18 -0.7 2,330,868 + RR42_RS30960 0.18 -0.2 2,330,869 - RR42_RS30960 0.18 +0.1 2,331,134 + RR42_RS30960 0.57 -1.2 2,331,134 + RR42_RS30960 0.57 +1.2 2,331,135 - RR42_RS30960 0.57 +0.3 2,331,291 - RR42_RS30960 0.80 +0.1 2,331,291 - RR42_RS30960 0.80 -0.5 2,331,291 - RR42_RS30960 0.80 -0.5 2,331,291 - RR42_RS30960 0.80 -0.9 2,331,291 - RR42_RS30960 0.80 -0.8 2,331,291 - RR42_RS30960 0.80 +0.2 2,331,380 + +0.0 2,331,380 + +0.0 2,331,381 - +1.4 2,331,381 - -0.0 2,331,415 + -1.0 2,331,415 + +0.0 2,331,415 + -0.5 2,331,416 - -0.6 2,331,423 + +0.4 2,331,423 + +0.5 2,331,424 - -0.3 2,331,424 - -0.1 2,331,428 - -0.9 2,331,428 - +0.4 2,331,471 - -0.2 2,331,471 - -0.9 2,331,471 - -2.4 2,331,471 - -0.4 2,331,472 + +2.0 2,331,473 - +0.7 2,331,473 - -2.6 2,331,549 + -0.9 2,331,550 - -0.4 2,331,554 + +1.1 2,331,594 + +1.9 2,331,595 - -0.2 2,331,595 - +0.3 2,331,598 + +1.1 2,331,598 + +0.4 2,331,599 - -1.4 2,331,599 - -0.1 2,331,757 - RR42_RS30965 0.32 +0.5 2,331,761 + RR42_RS30965 0.33 +1.5 2,331,762 - RR42_RS30965 0.33 -2.1 2,331,762 - RR42_RS30965 0.33 -0.6 2,331,762 - RR42_RS30965 0.33 -0.5 2,332,357 + RR42_RS30970 0.22 +3.1 2,332,357 + RR42_RS30970 0.22 -0.1 2,332,357 + RR42_RS30970 0.22 -0.8 2,332,361 + RR42_RS30970 0.23 -1.4 2,332,621 + RR42_RS30970 0.62 -0.6 2,332,621 + RR42_RS30970 0.62 -0.1 2,332,622 - RR42_RS30970 0.63 +1.3 2,332,622 - RR42_RS30970 0.63 +0.4 2,332,622 - RR42_RS30970 0.63 -0.8 2,332,667 - RR42_RS30970 0.69 -1.0 2,332,838 - -0.4 2,332,838 - -0.5 2,332,838 - -0.3 2,333,123 + RR42_RS30975 0.45 +0.1
Or see this region's nucleotide sequence