Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30035 and RR42_RS30040 overlap by 20 nucleotides RR42_RS30040 and RR42_RS30045 overlap by 14 nucleotides
RR42_RS30035: RR42_RS30035 - hemolysin D, at 2,110,210 to 2,111,259
_RS30035
RR42_RS30040: RR42_RS30040 - fusaric acid resistance protein, at 2,111,240 to 2,113,264
_RS30040
RR42_RS30045: RR42_RS30045 - RND transporter, at 2,113,251 to 2,114,789
_RS30045
Position (kb)
2111
2112
2113
2114 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2110.603 kb on + strand, within RR42_RS30035 at 2110.603 kb on + strand, within RR42_RS30035 at 2110.604 kb on - strand, within RR42_RS30035 at 2110.657 kb on + strand, within RR42_RS30035 at 2110.657 kb on + strand, within RR42_RS30035 at 2110.658 kb on - strand, within RR42_RS30035 at 2110.931 kb on + strand, within RR42_RS30035 at 2110.932 kb on - strand, within RR42_RS30035 at 2111.017 kb on + strand, within RR42_RS30035 at 2111.018 kb on - strand, within RR42_RS30035 at 2111.070 kb on + strand, within RR42_RS30035 at 2111.070 kb on + strand, within RR42_RS30035 at 2111.071 kb on - strand, within RR42_RS30035 at 2111.119 kb on + strand, within RR42_RS30035 at 2111.119 kb on + strand, within RR42_RS30035 at 2111.120 kb on - strand, within RR42_RS30035 at 2111.120 kb on - strand, within RR42_RS30035 at 2111.385 kb on - strand at 2111.385 kb on - strand at 2111.385 kb on - strand at 2111.385 kb on - strand at 2111.385 kb on - strand at 2111.385 kb on - strand at 2111.414 kb on + strand at 2111.414 kb on + strand at 2111.414 kb on + strand at 2111.415 kb on - strand at 2111.417 kb on - strand at 2111.417 kb on - strand at 2111.708 kb on + strand, within RR42_RS30040 at 2111.981 kb on - strand, within RR42_RS30040 at 2111.981 kb on - strand, within RR42_RS30040 at 2111.981 kb on - strand, within RR42_RS30040 at 2112.299 kb on - strand, within RR42_RS30040 at 2112.299 kb on - strand, within RR42_RS30040 at 2112.357 kb on - strand, within RR42_RS30040 at 2112.366 kb on - strand, within RR42_RS30040 at 2112.394 kb on + strand, within RR42_RS30040 at 2112.394 kb on + strand, within RR42_RS30040 at 2112.394 kb on + strand, within RR42_RS30040 at 2112.394 kb on + strand, within RR42_RS30040 at 2112.395 kb on - strand, within RR42_RS30040 at 2112.395 kb on - strand, within RR42_RS30040 at 2112.395 kb on - strand, within RR42_RS30040 at 2112.395 kb on - strand, within RR42_RS30040 at 2112.413 kb on + strand, within RR42_RS30040 at 2112.414 kb on - strand, within RR42_RS30040 at 2112.426 kb on + strand, within RR42_RS30040 at 2112.515 kb on + strand, within RR42_RS30040 at 2112.515 kb on + strand, within RR42_RS30040 at 2112.515 kb on + strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.516 kb on - strand, within RR42_RS30040 at 2112.597 kb on + strand, within RR42_RS30040 at 2112.617 kb on + strand, within RR42_RS30040 at 2112.647 kb on + strand, within RR42_RS30040 at 2112.648 kb on - strand, within RR42_RS30040 at 2112.648 kb on - strand, within RR42_RS30040 at 2112.648 kb on - strand, within RR42_RS30040 at 2112.741 kb on - strand, within RR42_RS30040 at 2112.741 kb on - strand, within RR42_RS30040 at 2113.242 kb on - strand at 2113.510 kb on - strand, within RR42_RS30045 at 2113.620 kb on + strand, within RR42_RS30045 at 2113.620 kb on + strand, within RR42_RS30045 at 2113.821 kb on - strand, within RR42_RS30045 at 2113.876 kb on - strand, within RR42_RS30045 at 2113.876 kb on - strand, within RR42_RS30045 at 2114.070 kb on + strand, within RR42_RS30045 at 2114.097 kb on - strand, within RR42_RS30045 at 2114.097 kb on - strand, within RR42_RS30045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,110,603 + RR42_RS30035 0.37 -0.7 2,110,603 + RR42_RS30035 0.37 +0.0 2,110,604 - RR42_RS30035 0.38 +0.2 2,110,657 + RR42_RS30035 0.43 -0.6 2,110,657 + RR42_RS30035 0.43 +0.7 2,110,658 - RR42_RS30035 0.43 -0.2 2,110,931 + RR42_RS30035 0.69 -0.3 2,110,932 - RR42_RS30035 0.69 -0.5 2,111,017 + RR42_RS30035 0.77 -1.3 2,111,018 - RR42_RS30035 0.77 -0.4 2,111,070 + RR42_RS30035 0.82 -2.9 2,111,070 + RR42_RS30035 0.82 -0.1 2,111,071 - RR42_RS30035 0.82 +0.4 2,111,119 + RR42_RS30035 0.87 +0.9 2,111,119 + RR42_RS30035 0.87 +0.4 2,111,120 - RR42_RS30035 0.87 -0.4 2,111,120 - RR42_RS30035 0.87 -1.2 2,111,385 - -0.2 2,111,385 - -0.2 2,111,385 - -0.3 2,111,385 - -0.3 2,111,385 - -0.4 2,111,385 - -0.9 2,111,414 + -0.2 2,111,414 + +1.0 2,111,414 + +0.6 2,111,415 - +0.7 2,111,417 - +0.1 2,111,417 - +0.4 2,111,708 + RR42_RS30040 0.23 -0.0 2,111,981 - RR42_RS30040 0.37 -0.7 2,111,981 - RR42_RS30040 0.37 +0.2 2,111,981 - RR42_RS30040 0.37 +0.4 2,112,299 - RR42_RS30040 0.52 -0.1 2,112,299 - RR42_RS30040 0.52 -0.7 2,112,357 - RR42_RS30040 0.55 -0.3 2,112,366 - RR42_RS30040 0.56 -0.3 2,112,394 + RR42_RS30040 0.57 +0.2 2,112,394 + RR42_RS30040 0.57 -0.9 2,112,394 + RR42_RS30040 0.57 -0.0 2,112,394 + RR42_RS30040 0.57 -0.4 2,112,395 - RR42_RS30040 0.57 -0.5 2,112,395 - RR42_RS30040 0.57 +0.2 2,112,395 - RR42_RS30040 0.57 -0.4 2,112,395 - RR42_RS30040 0.57 -0.2 2,112,413 + RR42_RS30040 0.58 -0.5 2,112,414 - RR42_RS30040 0.58 -0.1 2,112,426 + RR42_RS30040 0.59 +1.9 2,112,515 + RR42_RS30040 0.63 -3.6 2,112,515 + RR42_RS30040 0.63 -0.3 2,112,515 + RR42_RS30040 0.63 -0.3 2,112,516 - RR42_RS30040 0.63 -0.2 2,112,516 - RR42_RS30040 0.63 -0.3 2,112,516 - RR42_RS30040 0.63 +0.4 2,112,516 - RR42_RS30040 0.63 +0.1 2,112,516 - RR42_RS30040 0.63 -0.4 2,112,516 - RR42_RS30040 0.63 -0.2 2,112,516 - RR42_RS30040 0.63 +0.0 2,112,516 - RR42_RS30040 0.63 -0.4 2,112,597 + RR42_RS30040 0.67 -0.3 2,112,617 + RR42_RS30040 0.68 -0.3 2,112,647 + RR42_RS30040 0.69 +0.1 2,112,648 - RR42_RS30040 0.70 -1.7 2,112,648 - RR42_RS30040 0.70 +0.3 2,112,648 - RR42_RS30040 0.70 +0.2 2,112,741 - RR42_RS30040 0.74 +0.2 2,112,741 - RR42_RS30040 0.74 -0.7 2,113,242 - +0.8 2,113,510 - RR42_RS30045 0.17 +0.5 2,113,620 + RR42_RS30045 0.24 +0.2 2,113,620 + RR42_RS30045 0.24 +0.7 2,113,821 - RR42_RS30045 0.37 -0.1 2,113,876 - RR42_RS30045 0.41 +0.4 2,113,876 - RR42_RS30045 0.41 -0.2 2,114,070 + RR42_RS30045 0.53 +0.4 2,114,097 - RR42_RS30045 0.55 -0.1 2,114,097 - RR42_RS30045 0.55 +0.4
Or see this region's nucleotide sequence