Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28880 and RR42_RS28885 are separated by 257 nucleotides RR42_RS28885 and RR42_RS28890 are separated by 389 nucleotides
RR42_RS28880: RR42_RS28880 - hypothetical protein, at 1,832,213 to 1,833,547
_RS28880
RR42_RS28885: RR42_RS28885 - membrane protein, at 1,833,805 to 1,835,118
_RS28885
RR42_RS28890: RR42_RS28890 - acetone carboxylase subunit beta, at 1,835,508 to 1,837,652
_RS28890
Position (kb)
1833
1834
1835
1836 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1832.901 kb on + strand, within RR42_RS28880 at 1832.902 kb on - strand, within RR42_RS28880 at 1832.902 kb on - strand, within RR42_RS28880 at 1833.078 kb on + strand, within RR42_RS28880 at 1833.079 kb on - strand, within RR42_RS28880 at 1833.248 kb on + strand, within RR42_RS28880 at 1833.249 kb on - strand, within RR42_RS28880 at 1833.354 kb on - strand, within RR42_RS28880 at 1833.393 kb on + strand, within RR42_RS28880 at 1833.394 kb on - strand, within RR42_RS28880 at 1833.394 kb on - strand, within RR42_RS28880 at 1833.394 kb on - strand, within RR42_RS28880 at 1833.394 kb on - strand, within RR42_RS28880 at 1833.397 kb on + strand, within RR42_RS28880 at 1833.398 kb on - strand, within RR42_RS28880 at 1833.691 kb on + strand at 1833.703 kb on + strand at 1833.703 kb on + strand at 1833.704 kb on - strand at 1833.746 kb on + strand at 1833.746 kb on + strand at 1833.859 kb on + strand at 1833.859 kb on + strand at 1833.860 kb on - strand at 1834.169 kb on - strand, within RR42_RS28885 at 1834.515 kb on + strand, within RR42_RS28885 at 1834.515 kb on + strand, within RR42_RS28885 at 1834.516 kb on - strand, within RR42_RS28885 at 1834.516 kb on - strand, within RR42_RS28885 at 1834.516 kb on - strand, within RR42_RS28885 at 1834.661 kb on - strand, within RR42_RS28885 at 1834.790 kb on + strand, within RR42_RS28885 at 1834.790 kb on + strand, within RR42_RS28885 at 1834.791 kb on - strand, within RR42_RS28885 at 1834.947 kb on + strand, within RR42_RS28885 at 1835.149 kb on - strand at 1835.165 kb on + strand at 1835.165 kb on + strand at 1835.166 kb on - strand at 1835.167 kb on - strand at 1835.315 kb on + strand at 1835.316 kb on - strand at 1835.316 kb on - strand at 1835.372 kb on - strand at 1835.372 kb on - strand at 1835.507 kb on + strand at 1835.507 kb on + strand at 1835.507 kb on + strand at 1835.507 kb on + strand at 1835.508 kb on - strand at 1835.508 kb on - strand at 1835.508 kb on - strand at 1835.508 kb on - strand at 1835.698 kb on + strand at 1835.699 kb on - strand at 1835.699 kb on - strand at 1835.838 kb on + strand, within RR42_RS28890 at 1835.877 kb on - strand, within RR42_RS28890 at 1835.886 kb on + strand, within RR42_RS28890
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 1,832,901 + RR42_RS28880 0.52 +0.7 1,832,902 - RR42_RS28880 0.52 +0.1 1,832,902 - RR42_RS28880 0.52 -1.4 1,833,078 + RR42_RS28880 0.65 +0.6 1,833,079 - RR42_RS28880 0.65 -3.1 1,833,248 + RR42_RS28880 0.78 -1.3 1,833,249 - RR42_RS28880 0.78 -1.2 1,833,354 - RR42_RS28880 0.85 -0.5 1,833,393 + RR42_RS28880 0.88 +0.0 1,833,394 - RR42_RS28880 0.88 +0.5 1,833,394 - RR42_RS28880 0.88 +0.0 1,833,394 - RR42_RS28880 0.88 +0.9 1,833,394 - RR42_RS28880 0.88 +0.4 1,833,397 + RR42_RS28880 0.89 -0.0 1,833,398 - RR42_RS28880 0.89 +0.2 1,833,691 + +0.3 1,833,703 + -0.1 1,833,703 + -1.3 1,833,704 - -0.3 1,833,746 + -0.4 1,833,746 + -0.2 1,833,859 + -1.5 1,833,859 + -1.6 1,833,860 - +0.2 1,834,169 - RR42_RS28885 0.28 -2.9 1,834,515 + RR42_RS28885 0.54 -2.5 1,834,515 + RR42_RS28885 0.54 -1.9 1,834,516 - RR42_RS28885 0.54 -2.9 1,834,516 - RR42_RS28885 0.54 -2.9 1,834,516 - RR42_RS28885 0.54 -1.7 1,834,661 - RR42_RS28885 0.65 -0.9 1,834,790 + RR42_RS28885 0.75 -0.3 1,834,790 + RR42_RS28885 0.75 -1.6 1,834,791 - RR42_RS28885 0.75 -1.3 1,834,947 + RR42_RS28885 0.87 -0.9 1,835,149 - -1.3 1,835,165 + -0.0 1,835,165 + -1.2 1,835,166 - -0.2 1,835,167 - -1.7 1,835,315 + +0.7 1,835,316 - +0.4 1,835,316 - +0.7 1,835,372 - +0.8 1,835,372 - -0.5 1,835,507 + +0.3 1,835,507 + -0.2 1,835,507 + +0.2 1,835,507 + +0.5 1,835,508 - -0.5 1,835,508 - -0.7 1,835,508 - -0.5 1,835,508 - +0.4 1,835,698 + +0.2 1,835,699 - +1.3 1,835,699 - -0.2 1,835,838 + RR42_RS28890 0.15 -0.2 1,835,877 - RR42_RS28890 0.17 +1.2 1,835,886 + RR42_RS28890 0.18 +0.5
Or see this region's nucleotide sequence