Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28825 and RR42_RS28830 are separated by 7 nucleotides RR42_RS28830 and RR42_RS28835 are separated by 269 nucleotides RR42_RS28835 and RR42_RS28840 are separated by 116 nucleotides RR42_RS28840 and RR42_RS28845 are separated by 40 nucleotides
RR42_RS28825: RR42_RS28825 - energy transducer TonB, at 1,819,720 to 1,822,122
_RS28825
RR42_RS28830: RR42_RS28830 - hypothetical protein, at 1,822,130 to 1,822,462
_RS28830
RR42_RS28835: RR42_RS28835 - gluconate:H+ symporter (gntT) (from data) , at 1,822,732 to 1,824,093
_RS28835
RR42_RS28840: RR42_RS28840 - hypothetical protein, at 1,824,210 to 1,824,602
_RS28840
RR42_RS28845: RR42_RS28845 - D-aminoacylase, at 1,824,643 to 1,826,136
_RS28845
Position (kb)
1822
1823
1824
1825 Strain fitness (log2 ratio)
-1
0
1 at 1821.892 kb on + strand at 1821.907 kb on + strand at 1821.908 kb on - strand at 1821.978 kb on + strand at 1822.131 kb on + strand at 1822.131 kb on + strand at 1822.132 kb on - strand at 1822.221 kb on - strand, within RR42_RS28830 at 1822.278 kb on + strand, within RR42_RS28830 at 1822.279 kb on - strand, within RR42_RS28830 at 1822.279 kb on - strand, within RR42_RS28830 at 1822.456 kb on + strand at 1822.457 kb on - strand at 1822.494 kb on - strand at 1822.528 kb on - strand at 1822.631 kb on + strand at 1822.632 kb on - strand at 1822.739 kb on - strand at 1822.838 kb on + strand at 1822.839 kb on - strand at 1823.228 kb on + strand, within RR42_RS28835 at 1823.228 kb on + strand, within RR42_RS28835 at 1823.229 kb on - strand, within RR42_RS28835 at 1823.229 kb on - strand, within RR42_RS28835 at 1823.229 kb on - strand, within RR42_RS28835 at 1823.229 kb on - strand, within RR42_RS28835 at 1823.229 kb on - strand, within RR42_RS28835 at 1823.511 kb on + strand, within RR42_RS28835 at 1823.511 kb on + strand, within RR42_RS28835 at 1823.574 kb on - strand, within RR42_RS28835 at 1823.574 kb on - strand, within RR42_RS28835 at 1823.755 kb on - strand, within RR42_RS28835 at 1823.755 kb on - strand, within RR42_RS28835 at 1823.911 kb on + strand, within RR42_RS28835 at 1823.911 kb on + strand, within RR42_RS28835 at 1823.995 kb on - strand at 1824.059 kb on + strand at 1824.059 kb on + strand at 1824.229 kb on + strand at 1824.508 kb on + strand, within RR42_RS28840 at 1824.508 kb on + strand, within RR42_RS28840 at 1824.508 kb on + strand, within RR42_RS28840 at 1824.508 kb on + strand, within RR42_RS28840 at 1824.509 kb on - strand, within RR42_RS28840 at 1824.596 kb on + strand at 1824.636 kb on + strand at 1824.636 kb on + strand at 1824.636 kb on + strand at 1824.637 kb on - strand at 1824.637 kb on - strand at 1824.637 kb on - strand at 1824.637 kb on - strand at 1824.637 kb on - strand at 1824.637 kb on - strand at 1824.644 kb on + strand at 1824.645 kb on - strand at 1824.848 kb on + strand, within RR42_RS28845 at 1825.092 kb on - strand, within RR42_RS28845 at 1825.092 kb on - strand, within RR42_RS28845 at 1825.092 kb on - strand, within RR42_RS28845
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 1,821,892 + -0.8 1,821,907 + -0.1 1,821,908 - +0.5 1,821,978 + +0.3 1,822,131 + +0.4 1,822,131 + -0.5 1,822,132 - +0.3 1,822,221 - RR42_RS28830 0.27 -0.9 1,822,278 + RR42_RS28830 0.44 +0.3 1,822,279 - RR42_RS28830 0.45 -1.4 1,822,279 - RR42_RS28830 0.45 -0.0 1,822,456 + +0.6 1,822,457 - +0.2 1,822,494 - -0.5 1,822,528 - -0.9 1,822,631 + +0.6 1,822,632 - +0.2 1,822,739 - -0.0 1,822,838 + -0.5 1,822,839 - +1.1 1,823,228 + RR42_RS28835 0.36 +0.1 1,823,228 + RR42_RS28835 0.36 -1.2 1,823,229 - RR42_RS28835 0.36 +0.8 1,823,229 - RR42_RS28835 0.36 +0.5 1,823,229 - RR42_RS28835 0.36 +0.4 1,823,229 - RR42_RS28835 0.36 +1.0 1,823,229 - RR42_RS28835 0.36 -0.0 1,823,511 + RR42_RS28835 0.57 -0.5 1,823,511 + RR42_RS28835 0.57 +0.3 1,823,574 - RR42_RS28835 0.62 +1.3 1,823,574 - RR42_RS28835 0.62 +0.3 1,823,755 - RR42_RS28835 0.75 +0.3 1,823,755 - RR42_RS28835 0.75 +0.7 1,823,911 + RR42_RS28835 0.87 +0.2 1,823,911 + RR42_RS28835 0.87 +0.3 1,823,995 - -0.6 1,824,059 + +1.3 1,824,059 + +1.2 1,824,229 + -1.1 1,824,508 + RR42_RS28840 0.76 +0.0 1,824,508 + RR42_RS28840 0.76 -0.5 1,824,508 + RR42_RS28840 0.76 +0.7 1,824,508 + RR42_RS28840 0.76 +0.3 1,824,509 - RR42_RS28840 0.76 +0.1 1,824,596 + -0.0 1,824,636 + +0.6 1,824,636 + -0.5 1,824,636 + -0.5 1,824,637 - -0.2 1,824,637 - +0.3 1,824,637 - +0.5 1,824,637 - -0.9 1,824,637 - -0.8 1,824,637 - +0.1 1,824,644 + +0.8 1,824,645 - -0.7 1,824,848 + RR42_RS28845 0.14 +0.3 1,825,092 - RR42_RS28845 0.30 -1.0 1,825,092 - RR42_RS28845 0.30 +0.5 1,825,092 - RR42_RS28845 0.30 -0.2
Or see this region's nucleotide sequence