Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28555 and RR42_RS28560 are separated by 449 nucleotides RR42_RS28560 and RR42_RS28565 are separated by 76 nucleotides
RR42_RS28555: RR42_RS28555 - 3-hydroxyisobutyrate dehydrogenase, at 1,761,093 to 1,761,992
_RS28555
RR42_RS28560: RR42_RS28560 - methylmalonate-semialdehyde dehydrogenase, at 1,762,442 to 1,763,962
_RS28560
RR42_RS28565: RR42_RS28565 - acyl-CoA dehydrogenase, at 1,764,039 to 1,765,172
_RS28565
Position (kb)
1762
1763
1764 Strain fitness (log2 ratio)
-2
-1
0
1 at 1761.970 kb on - strand at 1761.984 kb on + strand at 1761.984 kb on + strand at 1761.984 kb on + strand at 1761.985 kb on - strand at 1762.042 kb on + strand at 1762.043 kb on - strand at 1762.462 kb on + strand at 1762.527 kb on + strand at 1762.551 kb on + strand at 1762.698 kb on - strand, within RR42_RS28560 at 1762.772 kb on + strand, within RR42_RS28560 at 1762.773 kb on - strand, within RR42_RS28560 at 1762.803 kb on + strand, within RR42_RS28560 at 1762.803 kb on + strand, within RR42_RS28560 at 1762.803 kb on + strand, within RR42_RS28560 at 1762.979 kb on + strand, within RR42_RS28560 at 1762.980 kb on - strand, within RR42_RS28560 at 1763.067 kb on - strand, within RR42_RS28560 at 1763.067 kb on - strand, within RR42_RS28560 at 1763.236 kb on - strand, within RR42_RS28560 at 1763.236 kb on - strand, within RR42_RS28560 at 1763.535 kb on + strand, within RR42_RS28560 at 1763.539 kb on + strand, within RR42_RS28560 at 1763.681 kb on - strand, within RR42_RS28560 at 1763.721 kb on + strand, within RR42_RS28560 at 1763.721 kb on + strand, within RR42_RS28560 at 1763.983 kb on + strand at 1763.983 kb on + strand at 1764.088 kb on + strand at 1764.134 kb on - strand at 1764.145 kb on + strand at 1764.209 kb on - strand, within RR42_RS28565 at 1764.209 kb on - strand, within RR42_RS28565 at 1764.209 kb on - strand, within RR42_RS28565 at 1764.209 kb on - strand, within RR42_RS28565 at 1764.209 kb on - strand, within RR42_RS28565 at 1764.845 kb on - strand, within RR42_RS28565
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 1,761,970 - +0.4 1,761,984 + -1.4 1,761,984 + +0.1 1,761,984 + -0.2 1,761,985 - +0.7 1,762,042 + +0.3 1,762,043 - -0.3 1,762,462 + +0.0 1,762,527 + +0.5 1,762,551 + -0.7 1,762,698 - RR42_RS28560 0.17 +0.2 1,762,772 + RR42_RS28560 0.22 -0.9 1,762,773 - RR42_RS28560 0.22 +1.5 1,762,803 + RR42_RS28560 0.24 -1.3 1,762,803 + RR42_RS28560 0.24 -0.3 1,762,803 + RR42_RS28560 0.24 -0.3 1,762,979 + RR42_RS28560 0.35 +0.2 1,762,980 - RR42_RS28560 0.35 -1.9 1,763,067 - RR42_RS28560 0.41 +0.4 1,763,067 - RR42_RS28560 0.41 +0.4 1,763,236 - RR42_RS28560 0.52 -1.2 1,763,236 - RR42_RS28560 0.52 -1.1 1,763,535 + RR42_RS28560 0.72 +0.2 1,763,539 + RR42_RS28560 0.72 -0.1 1,763,681 - RR42_RS28560 0.81 -0.3 1,763,721 + RR42_RS28560 0.84 -1.2 1,763,721 + RR42_RS28560 0.84 -0.5 1,763,983 + -0.4 1,763,983 + -0.7 1,764,088 + +0.8 1,764,134 - -0.3 1,764,145 + -0.6 1,764,209 - RR42_RS28565 0.15 +0.1 1,764,209 - RR42_RS28565 0.15 -0.3 1,764,209 - RR42_RS28565 0.15 +0.1 1,764,209 - RR42_RS28565 0.15 -0.2 1,764,209 - RR42_RS28565 0.15 -0.2 1,764,845 - RR42_RS28565 0.71 +0.3
Or see this region's nucleotide sequence