Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27835 and RR42_RS27840 are separated by 37 nucleotides RR42_RS27840 and RR42_RS27845 are separated by 65 nucleotides RR42_RS27845 and RR42_RS27850 are separated by 63 nucleotides RR42_RS27850 and RR42_RS27855 are separated by 21 nucleotides
RR42_RS27835: RR42_RS27835 - betaine-aldehyde dehydrogenase, at 1,608,708 to 1,610,168
_RS27835
RR42_RS27840: RR42_RS27840 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 1,610,206 to 1,611,054
_RS27840
RR42_RS27845: RR42_RS27845 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 1,611,120 to 1,611,509
_RS27845
RR42_RS27850: RR42_RS27850 - 2-oxo-hepta-3-ene-1,7-dioate hydratase, at 1,611,573 to 1,612,376
_RS27850
RR42_RS27855: RR42_RS27855 - 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase, at 1,612,398 to 1,613,201
_RS27855
Position (kb) 1611
1612 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1610.147 kb on + strand at 1610.148 kb on - strand at 1610.216 kb on + strand at 1610.216 kb on + strand at 1610.431 kb on + strand, within RR42_RS27840 at 1610.431 kb on + strand, within RR42_RS27840 at 1610.431 kb on + strand, within RR42_RS27840 at 1610.431 kb on + strand, within RR42_RS27840 at 1610.474 kb on - strand, within RR42_RS27840 at 1610.603 kb on - strand, within RR42_RS27840 at 1610.897 kb on - strand, within RR42_RS27840 at 1610.972 kb on - strand at 1610.973 kb on - strand at 1611.142 kb on - strand at 1611.298 kb on - strand, within RR42_RS27845 at 1611.298 kb on - strand, within RR42_RS27845 at 1611.406 kb on + strand, within RR42_RS27845 at 1611.406 kb on + strand, within RR42_RS27845 at 1611.406 kb on + strand, within RR42_RS27845 at 1611.407 kb on - strand, within RR42_RS27845 at 1611.409 kb on - strand, within RR42_RS27845 at 1611.409 kb on - strand, within RR42_RS27845 at 1611.468 kb on + strand, within RR42_RS27845 at 1611.690 kb on + strand, within RR42_RS27850 at 1611.811 kb on - strand, within RR42_RS27850 at 1611.954 kb on + strand, within RR42_RS27850 at 1611.954 kb on + strand, within RR42_RS27850 at 1611.955 kb on - strand, within RR42_RS27850 at 1611.969 kb on + strand, within RR42_RS27850 at 1611.969 kb on + strand, within RR42_RS27850 at 1612.322 kb on + strand at 1612.415 kb on + strand at 1612.482 kb on + strand, within RR42_RS27855 at 1612.483 kb on - strand, within RR42_RS27855
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 1,610,147 + -0.1 1,610,148 - +0.1 1,610,216 + +0.6 1,610,216 + +0.6 1,610,431 + RR42_RS27840 0.27 -0.7 1,610,431 + RR42_RS27840 0.27 -0.4 1,610,431 + RR42_RS27840 0.27 -0.2 1,610,431 + RR42_RS27840 0.27 +0.1 1,610,474 - RR42_RS27840 0.32 -1.7 1,610,603 - RR42_RS27840 0.47 -0.7 1,610,897 - RR42_RS27840 0.81 +0.5 1,610,972 - -1.0 1,610,973 - -1.4 1,611,142 - +3.1 1,611,298 - RR42_RS27845 0.46 +0.6 1,611,298 - RR42_RS27845 0.46 -3.0 1,611,406 + RR42_RS27845 0.73 +0.4 1,611,406 + RR42_RS27845 0.73 +0.0 1,611,406 + RR42_RS27845 0.73 -0.6 1,611,407 - RR42_RS27845 0.74 +0.9 1,611,409 - RR42_RS27845 0.74 -0.4 1,611,409 - RR42_RS27845 0.74 -0.3 1,611,468 + RR42_RS27845 0.89 +0.9 1,611,690 + RR42_RS27850 0.15 +0.4 1,611,811 - RR42_RS27850 0.30 +0.7 1,611,954 + RR42_RS27850 0.47 +0.6 1,611,954 + RR42_RS27850 0.47 -0.5 1,611,955 - RR42_RS27850 0.48 +0.7 1,611,969 + RR42_RS27850 0.49 -0.4 1,611,969 + RR42_RS27850 0.49 +0.2 1,612,322 + +0.2 1,612,415 + -0.0 1,612,482 + RR42_RS27855 0.10 -0.9 1,612,483 - RR42_RS27855 0.11 +0.4
Or see this region's nucleotide sequence