Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27675 and RR42_RS27680 are separated by 143 nucleotides RR42_RS27680 and RR42_RS27685 are separated by 223 nucleotides
RR42_RS27675: RR42_RS27675 - esterase, at 1,571,007 to 1,571,930
_RS27675
RR42_RS27680: RR42_RS27680 - MexE family multidrug efflux RND transporter periplasmic adaptor subunit, at 1,572,074 to 1,573,345
_RS27680
RR42_RS27685: RR42_RS27685 - multidrug efflux RND transporter permease subunit, at 1,573,569 to 1,576,760
_RS27685
Position (kb)
1572
1573
1574 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1571.125 kb on - strand, within RR42_RS27675 at 1571.178 kb on + strand, within RR42_RS27675 at 1571.178 kb on + strand, within RR42_RS27675 at 1571.178 kb on + strand, within RR42_RS27675 at 1571.586 kb on + strand, within RR42_RS27675 at 1571.587 kb on - strand, within RR42_RS27675 at 1571.590 kb on - strand, within RR42_RS27675 at 1571.598 kb on + strand, within RR42_RS27675 at 1571.632 kb on - strand, within RR42_RS27675 at 1571.940 kb on - strand at 1572.395 kb on + strand, within RR42_RS27680 at 1572.611 kb on + strand, within RR42_RS27680 at 1572.612 kb on - strand, within RR42_RS27680 at 1572.612 kb on - strand, within RR42_RS27680 at 1572.612 kb on - strand, within RR42_RS27680 at 1572.613 kb on + strand, within RR42_RS27680 at 1572.718 kb on - strand, within RR42_RS27680 at 1572.734 kb on + strand, within RR42_RS27680 at 1572.735 kb on - strand, within RR42_RS27680 at 1572.773 kb on + strand, within RR42_RS27680 at 1572.863 kb on + strand, within RR42_RS27680 at 1572.977 kb on + strand, within RR42_RS27680 at 1572.977 kb on + strand, within RR42_RS27680 at 1572.978 kb on - strand, within RR42_RS27680 at 1572.978 kb on - strand, within RR42_RS27680 at 1572.978 kb on - strand, within RR42_RS27680 at 1573.100 kb on + strand, within RR42_RS27680 at 1573.464 kb on + strand at 1573.464 kb on + strand at 1573.465 kb on - strand at 1573.601 kb on + strand at 1573.602 kb on - strand at 1573.602 kb on - strand at 1573.663 kb on + strand at 1573.803 kb on + strand at 1573.803 kb on + strand at 1573.803 kb on + strand at 1573.803 kb on + strand at 1573.803 kb on + strand at 1573.804 kb on - strand at 1573.804 kb on - strand at 1573.804 kb on - strand at 1573.804 kb on - strand at 1573.804 kb on - strand at 1573.804 kb on - strand at 1573.804 kb on - strand at 1573.823 kb on + strand at 1574.031 kb on + strand, within RR42_RS27685 at 1574.032 kb on - strand, within RR42_RS27685 at 1574.032 kb on - strand, within RR42_RS27685 at 1574.032 kb on - strand, within RR42_RS27685 at 1574.043 kb on + strand, within RR42_RS27685 at 1574.044 kb on - strand, within RR42_RS27685 at 1574.122 kb on - strand, within RR42_RS27685 at 1574.122 kb on - strand, within RR42_RS27685
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 1,571,125 - RR42_RS27675 0.13 +0.3 1,571,178 + RR42_RS27675 0.19 -0.4 1,571,178 + RR42_RS27675 0.19 -1.0 1,571,178 + RR42_RS27675 0.19 +1.2 1,571,586 + RR42_RS27675 0.63 -4.3 1,571,587 - RR42_RS27675 0.63 -0.9 1,571,590 - RR42_RS27675 0.63 +0.2 1,571,598 + RR42_RS27675 0.64 -0.7 1,571,632 - RR42_RS27675 0.68 +0.4 1,571,940 - +0.8 1,572,395 + RR42_RS27680 0.25 +0.0 1,572,611 + RR42_RS27680 0.42 -0.0 1,572,612 - RR42_RS27680 0.42 +0.4 1,572,612 - RR42_RS27680 0.42 -1.6 1,572,612 - RR42_RS27680 0.42 +0.6 1,572,613 + RR42_RS27680 0.42 -0.2 1,572,718 - RR42_RS27680 0.51 +0.5 1,572,734 + RR42_RS27680 0.52 -0.3 1,572,735 - RR42_RS27680 0.52 +0.0 1,572,773 + RR42_RS27680 0.55 +0.2 1,572,863 + RR42_RS27680 0.62 -0.3 1,572,977 + RR42_RS27680 0.71 -0.0 1,572,977 + RR42_RS27680 0.71 -1.8 1,572,978 - RR42_RS27680 0.71 +0.5 1,572,978 - RR42_RS27680 0.71 +0.6 1,572,978 - RR42_RS27680 0.71 -0.3 1,573,100 + RR42_RS27680 0.81 -0.7 1,573,464 + -0.0 1,573,464 + -0.3 1,573,465 - -0.5 1,573,601 + -0.1 1,573,602 - -0.2 1,573,602 - +0.5 1,573,663 + -0.4 1,573,803 + -0.2 1,573,803 + +0.3 1,573,803 + -1.7 1,573,803 + -0.7 1,573,803 + +0.1 1,573,804 - +0.5 1,573,804 - -0.1 1,573,804 - -0.7 1,573,804 - +0.3 1,573,804 - -0.5 1,573,804 - +1.1 1,573,804 - -0.8 1,573,823 + +0.3 1,574,031 + RR42_RS27685 0.14 -0.5 1,574,032 - RR42_RS27685 0.15 -1.0 1,574,032 - RR42_RS27685 0.15 +0.3 1,574,032 - RR42_RS27685 0.15 -0.8 1,574,043 + RR42_RS27685 0.15 -0.4 1,574,044 - RR42_RS27685 0.15 -1.0 1,574,122 - RR42_RS27685 0.17 +0.3 1,574,122 - RR42_RS27685 0.17 -1.6
Or see this region's nucleotide sequence