Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27635 and RR42_RS27640 are separated by 470 nucleotides RR42_RS27640 and RR42_RS27645 are separated by 89 nucleotides RR42_RS27645 and RR42_RS27650 are separated by 75 nucleotides
RR42_RS27635: RR42_RS27635 - hypothetical protein, at 1,564,308 to 1,564,529
_RS27635
RR42_RS27640: RR42_RS27640 - CDP-diacylglycerol pyrophosphatase, at 1,565,000 to 1,565,776
_RS27640
RR42_RS27645: RR42_RS27645 - hypothetical protein, at 1,565,866 to 1,566,120
_RS27645
RR42_RS27650: RR42_RS27650 - phosphate-binding protein, at 1,566,196 to 1,567,179
_RS27650
Position (kb)
1564
1565
1566 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1564.262 kb on + strand at 1564.263 kb on - strand at 1564.423 kb on + strand, within RR42_RS27635 at 1564.718 kb on + strand at 1564.718 kb on + strand at 1564.718 kb on + strand at 1564.719 kb on - strand at 1564.780 kb on + strand at 1564.780 kb on + strand at 1564.781 kb on - strand at 1564.781 kb on - strand at 1564.815 kb on + strand at 1565.013 kb on + strand at 1565.013 kb on + strand at 1565.014 kb on - strand at 1565.175 kb on - strand, within RR42_RS27640 at 1565.395 kb on + strand, within RR42_RS27640 at 1565.455 kb on + strand, within RR42_RS27640 at 1565.499 kb on + strand, within RR42_RS27640 at 1565.499 kb on + strand, within RR42_RS27640 at 1565.618 kb on + strand, within RR42_RS27640 at 1565.618 kb on + strand, within RR42_RS27640 at 1565.619 kb on - strand, within RR42_RS27640 at 1565.619 kb on - strand, within RR42_RS27640 at 1565.619 kb on - strand, within RR42_RS27640 at 1565.619 kb on - strand, within RR42_RS27640 at 1565.619 kb on - strand, within RR42_RS27640 at 1565.775 kb on - strand at 1565.827 kb on + strand at 1565.876 kb on - strand at 1565.876 kb on - strand at 1566.053 kb on - strand, within RR42_RS27645 at 1566.067 kb on + strand, within RR42_RS27645 at 1566.068 kb on - strand, within RR42_RS27645 at 1566.260 kb on + strand at 1566.261 kb on - strand at 1566.425 kb on + strand, within RR42_RS27650 at 1566.426 kb on - strand, within RR42_RS27650 at 1566.551 kb on + strand, within RR42_RS27650 at 1566.551 kb on + strand, within RR42_RS27650 at 1566.566 kb on + strand, within RR42_RS27650 at 1566.567 kb on - strand, within RR42_RS27650 at 1566.596 kb on + strand, within RR42_RS27650 at 1566.596 kb on + strand, within RR42_RS27650 at 1566.597 kb on - strand, within RR42_RS27650 at 1566.701 kb on + strand, within RR42_RS27650
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 1,564,262 + -1.2 1,564,263 - +0.5 1,564,423 + RR42_RS27635 0.52 -0.1 1,564,718 + +0.1 1,564,718 + -1.1 1,564,718 + +0.5 1,564,719 - -0.9 1,564,780 + +0.5 1,564,780 + +0.4 1,564,781 - -0.5 1,564,781 - +1.8 1,564,815 + -0.9 1,565,013 + +0.9 1,565,013 + -0.1 1,565,014 - -0.1 1,565,175 - RR42_RS27640 0.23 +0.1 1,565,395 + RR42_RS27640 0.51 +1.0 1,565,455 + RR42_RS27640 0.59 +0.6 1,565,499 + RR42_RS27640 0.64 +0.3 1,565,499 + RR42_RS27640 0.64 -0.7 1,565,618 + RR42_RS27640 0.80 +0.4 1,565,618 + RR42_RS27640 0.80 -0.4 1,565,619 - RR42_RS27640 0.80 +1.8 1,565,619 - RR42_RS27640 0.80 +0.2 1,565,619 - RR42_RS27640 0.80 +0.2 1,565,619 - RR42_RS27640 0.80 +0.4 1,565,619 - RR42_RS27640 0.80 -0.3 1,565,775 - +0.8 1,565,827 + -0.1 1,565,876 - +0.1 1,565,876 - +0.7 1,566,053 - RR42_RS27645 0.73 +0.5 1,566,067 + RR42_RS27645 0.79 -1.8 1,566,068 - RR42_RS27645 0.79 -0.0 1,566,260 + -1.5 1,566,261 - +1.5 1,566,425 + RR42_RS27650 0.23 -0.1 1,566,426 - RR42_RS27650 0.23 -1.2 1,566,551 + RR42_RS27650 0.36 +0.4 1,566,551 + RR42_RS27650 0.36 +0.2 1,566,566 + RR42_RS27650 0.38 -0.5 1,566,567 - RR42_RS27650 0.38 -1.4 1,566,596 + RR42_RS27650 0.41 +0.5 1,566,596 + RR42_RS27650 0.41 +0.5 1,566,597 - RR42_RS27650 0.41 +0.6 1,566,701 + RR42_RS27650 0.51 +0.7
Or see this region's nucleotide sequence