Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25505 and RR42_RS25510 are separated by 199 nucleotides RR42_RS25510 and RR42_RS25515 are separated by 11 nucleotides
RR42_RS25505: RR42_RS25505 - membrane protein, at 1,068,166 to 1,068,867
_RS25505
RR42_RS25510: RR42_RS25510 - short-chain dehydrogenase, at 1,069,067 to 1,069,855
_RS25510
RR42_RS25515: RR42_RS25515 - AMP-dependent synthetase, at 1,069,867 to 1,071,327
_RS25515
Position (kb) 1069
1070 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1068.174 kb on + strand at 1068.175 kb on - strand at 1068.175 kb on - strand at 1068.175 kb on - strand at 1068.176 kb on + strand at 1068.176 kb on + strand at 1068.177 kb on - strand at 1068.177 kb on - strand at 1068.177 kb on - strand at 1068.177 kb on - strand at 1068.196 kb on + strand at 1068.243 kb on + strand, within RR42_RS25505 at 1068.303 kb on + strand, within RR42_RS25505 at 1068.303 kb on + strand, within RR42_RS25505 at 1068.353 kb on + strand, within RR42_RS25505 at 1068.400 kb on + strand, within RR42_RS25505 at 1068.414 kb on - strand, within RR42_RS25505 at 1068.449 kb on + strand, within RR42_RS25505 at 1068.449 kb on + strand, within RR42_RS25505 at 1068.450 kb on - strand, within RR42_RS25505 at 1068.488 kb on - strand, within RR42_RS25505 at 1068.503 kb on + strand, within RR42_RS25505 at 1068.503 kb on + strand, within RR42_RS25505 at 1068.554 kb on - strand, within RR42_RS25505 at 1068.752 kb on + strand, within RR42_RS25505 at 1068.752 kb on + strand, within RR42_RS25505 at 1068.753 kb on - strand, within RR42_RS25505 at 1069.081 kb on - strand at 1069.123 kb on + strand at 1069.395 kb on + strand, within RR42_RS25510 at 1069.395 kb on + strand, within RR42_RS25510 at 1069.419 kb on + strand, within RR42_RS25510 at 1069.973 kb on + strand at 1070.150 kb on + strand, within RR42_RS25515 at 1070.150 kb on + strand, within RR42_RS25515 at 1070.151 kb on - strand, within RR42_RS25515 at 1070.501 kb on + strand, within RR42_RS25515 at 1070.501 kb on + strand, within RR42_RS25515 at 1070.501 kb on + strand, within RR42_RS25515 at 1070.502 kb on - strand, within RR42_RS25515 at 1070.618 kb on + strand, within RR42_RS25515 at 1070.619 kb on - strand, within RR42_RS25515 at 1070.642 kb on + strand, within RR42_RS25515 at 1070.643 kb on - strand, within RR42_RS25515 at 1070.643 kb on - strand, within RR42_RS25515
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 1,068,174 + +0.2 1,068,175 - -0.1 1,068,175 - +0.5 1,068,175 - -0.1 1,068,176 + -0.6 1,068,176 + +0.1 1,068,177 - +0.5 1,068,177 - +0.4 1,068,177 - +0.4 1,068,177 - -0.2 1,068,196 + -0.0 1,068,243 + RR42_RS25505 0.11 -1.1 1,068,303 + RR42_RS25505 0.20 +0.6 1,068,303 + RR42_RS25505 0.20 +0.3 1,068,353 + RR42_RS25505 0.27 +0.9 1,068,400 + RR42_RS25505 0.33 -2.8 1,068,414 - RR42_RS25505 0.35 -0.9 1,068,449 + RR42_RS25505 0.40 +2.9 1,068,449 + RR42_RS25505 0.40 +0.1 1,068,450 - RR42_RS25505 0.40 +0.5 1,068,488 - RR42_RS25505 0.46 +0.4 1,068,503 + RR42_RS25505 0.48 +0.1 1,068,503 + RR42_RS25505 0.48 +0.4 1,068,554 - RR42_RS25505 0.55 -0.2 1,068,752 + RR42_RS25505 0.83 -0.4 1,068,752 + RR42_RS25505 0.83 -0.4 1,068,753 - RR42_RS25505 0.84 +0.6 1,069,081 - -0.7 1,069,123 + +1.1 1,069,395 + RR42_RS25510 0.42 +0.1 1,069,395 + RR42_RS25510 0.42 -1.3 1,069,419 + RR42_RS25510 0.45 -1.4 1,069,973 + +0.6 1,070,150 + RR42_RS25515 0.19 +0.4 1,070,150 + RR42_RS25515 0.19 +0.2 1,070,151 - RR42_RS25515 0.19 +0.3 1,070,501 + RR42_RS25515 0.43 +0.4 1,070,501 + RR42_RS25515 0.43 -0.5 1,070,501 + RR42_RS25515 0.43 -1.3 1,070,502 - RR42_RS25515 0.43 +0.4 1,070,618 + RR42_RS25515 0.51 +0.6 1,070,619 - RR42_RS25515 0.51 +0.1 1,070,642 + RR42_RS25515 0.53 +0.1 1,070,643 - RR42_RS25515 0.53 +0.3 1,070,643 - RR42_RS25515 0.53 -0.1
Or see this region's nucleotide sequence