Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24630 and RR42_RS24635 are separated by 44 nucleotides RR42_RS24635 and RR42_RS24640 are separated by 34 nucleotides RR42_RS24640 and RR42_RS24645 overlap by 1 nucleotides RR42_RS24645 and RR42_RS24650 are separated by 245 nucleotides
RR42_RS24630: RR42_RS24630 - amine oxidase, at 862,468 to 863,964
_RS24630
RR42_RS24635: RR42_RS24635 - hypothetical protein, at 864,009 to 864,650
_RS24635
RR42_RS24640: RR42_RS24640 - amine oxidase, at 864,685 to 866,181
_RS24640
RR42_RS24645: RR42_RS24645 - hypothetical protein, at 866,181 to 866,861
_RS24645
RR42_RS24650: RR42_RS24650 - membrane protein, at 867,107 to 867,796
_RS24650
Position (kb)
864
865
866
867 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 863.689 kb on + strand, within RR42_RS24630 at 863.731 kb on + strand, within RR42_RS24630 at 863.732 kb on - strand, within RR42_RS24630 at 863.732 kb on - strand, within RR42_RS24630 at 863.887 kb on + strand at 863.888 kb on - strand at 863.970 kb on - strand at 864.037 kb on + strand at 864.038 kb on - strand at 864.066 kb on + strand at 864.082 kb on - strand, within RR42_RS24635 at 864.189 kb on + strand, within RR42_RS24635 at 864.216 kb on + strand, within RR42_RS24635 at 864.217 kb on - strand, within RR42_RS24635 at 864.489 kb on + strand, within RR42_RS24635 at 864.520 kb on - strand, within RR42_RS24635 at 864.615 kb on - strand at 864.634 kb on - strand at 864.634 kb on - strand at 864.671 kb on - strand at 864.671 kb on - strand at 864.694 kb on - strand at 864.757 kb on - strand at 864.963 kb on + strand, within RR42_RS24640 at 865.095 kb on + strand, within RR42_RS24640 at 865.402 kb on + strand, within RR42_RS24640 at 865.402 kb on + strand, within RR42_RS24640 at 865.402 kb on + strand, within RR42_RS24640 at 865.724 kb on - strand, within RR42_RS24640 at 865.799 kb on - strand, within RR42_RS24640 at 865.906 kb on + strand, within RR42_RS24640 at 865.907 kb on - strand, within RR42_RS24640 at 865.907 kb on - strand, within RR42_RS24640 at 865.907 kb on - strand, within RR42_RS24640 at 865.977 kb on + strand, within RR42_RS24640 at 865.978 kb on - strand, within RR42_RS24640 at 866.029 kb on + strand, within RR42_RS24640 at 866.029 kb on + strand, within RR42_RS24640 at 866.104 kb on + strand at 866.105 kb on - strand at 866.278 kb on - strand, within RR42_RS24645 at 866.293 kb on - strand, within RR42_RS24645 at 866.308 kb on + strand, within RR42_RS24645 at 866.308 kb on + strand, within RR42_RS24645 at 866.309 kb on - strand, within RR42_RS24645 at 866.457 kb on + strand, within RR42_RS24645 at 866.457 kb on + strand, within RR42_RS24645 at 866.457 kb on + strand, within RR42_RS24645 at 866.458 kb on - strand, within RR42_RS24645 at 866.700 kb on + strand, within RR42_RS24645 at 866.968 kb on - strand at 866.968 kb on - strand at 866.986 kb on + strand at 867.037 kb on + strand at 867.170 kb on + strand at 867.170 kb on + strand at 867.170 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 863,689 + RR42_RS24630 0.82 +0.0 863,731 + RR42_RS24630 0.84 +0.1 863,732 - RR42_RS24630 0.84 -0.4 863,732 - RR42_RS24630 0.84 -0.2 863,887 + -0.3 863,888 - +0.7 863,970 - -0.1 864,037 + -0.8 864,038 - -0.9 864,066 + +0.9 864,082 - RR42_RS24635 0.11 -0.6 864,189 + RR42_RS24635 0.28 -1.1 864,216 + RR42_RS24635 0.32 -0.3 864,217 - RR42_RS24635 0.32 -0.5 864,489 + RR42_RS24635 0.75 -0.3 864,520 - RR42_RS24635 0.80 -0.3 864,615 - -0.2 864,634 - -0.5 864,634 - -1.4 864,671 - -0.1 864,671 - -0.5 864,694 - -0.7 864,757 - +0.1 864,963 + RR42_RS24640 0.19 -0.1 865,095 + RR42_RS24640 0.27 -0.9 865,402 + RR42_RS24640 0.48 -2.8 865,402 + RR42_RS24640 0.48 -1.0 865,402 + RR42_RS24640 0.48 -1.1 865,724 - RR42_RS24640 0.69 -0.3 865,799 - RR42_RS24640 0.74 -0.4 865,906 + RR42_RS24640 0.82 -0.8 865,907 - RR42_RS24640 0.82 +0.3 865,907 - RR42_RS24640 0.82 +0.1 865,907 - RR42_RS24640 0.82 -1.0 865,977 + RR42_RS24640 0.86 -0.0 865,978 - RR42_RS24640 0.86 +0.7 866,029 + RR42_RS24640 0.90 -0.5 866,029 + RR42_RS24640 0.90 +0.2 866,104 + +0.0 866,105 - -0.6 866,278 - RR42_RS24645 0.14 -0.9 866,293 - RR42_RS24645 0.16 +0.2 866,308 + RR42_RS24645 0.19 -0.6 866,308 + RR42_RS24645 0.19 +0.0 866,309 - RR42_RS24645 0.19 -0.9 866,457 + RR42_RS24645 0.41 -1.1 866,457 + RR42_RS24645 0.41 -0.2 866,457 + RR42_RS24645 0.41 +0.1 866,458 - RR42_RS24645 0.41 +0.8 866,700 + RR42_RS24645 0.76 -1.2 866,968 - -0.0 866,968 - -0.2 866,986 + +0.3 867,037 + +0.1 867,170 + +0.2 867,170 + +0.1 867,170 + -0.0
Or see this region's nucleotide sequence