Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24555 and RR42_RS24560 are separated by 35 nucleotides RR42_RS24560 and RR42_RS24565 are separated by 208 nucleotides
RR42_RS24555: RR42_RS24555 - succinate-semialdehyde dehydrogenase, at 840,923 to 842,407
_RS24555
RR42_RS24560: RR42_RS24560 - alcohol dehydrogenase, at 842,443 to 843,612
_RS24560
RR42_RS24565: RR42_RS24565 - monooxygenase, at 843,821 to 845,275
_RS24565
Position (kb) 842
843
844 Strain fitness (log2 ratio) -2
-1
0
1 at 841.490 kb on - strand, within RR42_RS24555 at 841.862 kb on + strand, within RR42_RS24555 at 841.862 kb on + strand, within RR42_RS24555 at 841.863 kb on - strand, within RR42_RS24555 at 842.130 kb on + strand, within RR42_RS24555 at 842.192 kb on + strand, within RR42_RS24555 at 842.192 kb on + strand, within RR42_RS24555 at 842.213 kb on + strand, within RR42_RS24555 at 842.213 kb on + strand, within RR42_RS24555 at 842.261 kb on + strand at 842.372 kb on + strand at 842.373 kb on - strand at 842.388 kb on - strand at 842.388 kb on - strand at 842.388 kb on - strand at 842.466 kb on - strand at 842.818 kb on + strand, within RR42_RS24560 at 842.818 kb on + strand, within RR42_RS24560 at 842.818 kb on + strand, within RR42_RS24560 at 842.818 kb on + strand, within RR42_RS24560 at 842.818 kb on + strand, within RR42_RS24560 at 842.819 kb on - strand, within RR42_RS24560 at 842.819 kb on - strand, within RR42_RS24560 at 842.902 kb on - strand, within RR42_RS24560 at 842.902 kb on - strand, within RR42_RS24560 at 842.960 kb on - strand, within RR42_RS24560 at 843.062 kb on - strand, within RR42_RS24560 at 843.062 kb on - strand, within RR42_RS24560 at 843.062 kb on - strand, within RR42_RS24560 at 843.125 kb on + strand, within RR42_RS24560 at 843.141 kb on - strand, within RR42_RS24560 at 843.257 kb on - strand, within RR42_RS24560 at 843.427 kb on - strand, within RR42_RS24560 at 843.546 kb on - strand at 843.607 kb on + strand at 843.746 kb on + strand at 843.746 kb on + strand at 843.747 kb on - strand at 843.747 kb on - strand at 843.763 kb on - strand at 843.840 kb on - strand at 843.840 kb on - strand at 843.972 kb on - strand, within RR42_RS24565 at 844.007 kb on + strand, within RR42_RS24565 at 844.008 kb on - strand, within RR42_RS24565 at 844.049 kb on + strand, within RR42_RS24565 at 844.076 kb on + strand, within RR42_RS24565 at 844.292 kb on + strand, within RR42_RS24565 at 844.293 kb on - strand, within RR42_RS24565 at 844.293 kb on - strand, within RR42_RS24565 at 844.379 kb on + strand, within RR42_RS24565 at 844.380 kb on - strand, within RR42_RS24565
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 841,490 - RR42_RS24555 0.38 +0.1 841,862 + RR42_RS24555 0.63 -0.6 841,862 + RR42_RS24555 0.63 -0.4 841,863 - RR42_RS24555 0.63 +0.1 842,130 + RR42_RS24555 0.81 -0.1 842,192 + RR42_RS24555 0.85 -0.2 842,192 + RR42_RS24555 0.85 -0.7 842,213 + RR42_RS24555 0.87 +0.4 842,213 + RR42_RS24555 0.87 -0.2 842,261 + +0.1 842,372 + +1.6 842,373 - -0.3 842,388 - +1.6 842,388 - +1.4 842,388 - +0.1 842,466 - -1.8 842,818 + RR42_RS24560 0.32 -0.9 842,818 + RR42_RS24560 0.32 -0.8 842,818 + RR42_RS24560 0.32 +0.8 842,818 + RR42_RS24560 0.32 -1.4 842,818 + RR42_RS24560 0.32 -1.1 842,819 - RR42_RS24560 0.32 -0.5 842,819 - RR42_RS24560 0.32 -0.2 842,902 - RR42_RS24560 0.39 -2.4 842,902 - RR42_RS24560 0.39 -0.8 842,960 - RR42_RS24560 0.44 -1.3 843,062 - RR42_RS24560 0.53 -0.3 843,062 - RR42_RS24560 0.53 -0.1 843,062 - RR42_RS24560 0.53 +0.2 843,125 + RR42_RS24560 0.58 -1.6 843,141 - RR42_RS24560 0.60 +0.0 843,257 - RR42_RS24560 0.70 +0.0 843,427 - RR42_RS24560 0.84 +0.4 843,546 - +0.3 843,607 + -1.2 843,746 + +0.2 843,746 + -0.5 843,747 - +1.1 843,747 - -0.4 843,763 - +0.2 843,840 - +0.1 843,840 - -0.7 843,972 - RR42_RS24565 0.10 -0.2 844,007 + RR42_RS24565 0.13 -1.6 844,008 - RR42_RS24565 0.13 -0.2 844,049 + RR42_RS24565 0.16 -0.5 844,076 + RR42_RS24565 0.18 +0.4 844,292 + RR42_RS24565 0.32 +0.0 844,293 - RR42_RS24565 0.32 +0.1 844,293 - RR42_RS24565 0.32 -0.1 844,379 + RR42_RS24565 0.38 +0.1 844,380 - RR42_RS24565 0.38 -0.2
Or see this region's nucleotide sequence