Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24265 and RR42_RS24270 are separated by 98 nucleotides RR42_RS24270 and RR42_RS24275 are separated by 108 nucleotides RR42_RS24275 and RR42_RS24280 overlap by 4 nucleotides
RR42_RS24265: RR42_RS24265 - hypothetical protein, at 771,699 to 772,415
_RS24265
RR42_RS24270: RR42_RS24270 - hypothetical protein, at 772,514 to 773,482
_RS24270
RR42_RS24275: RR42_RS24275 - hypothetical protein, at 773,591 to 774,478
_RS24275
RR42_RS24280: RR42_RS24280 - gentisate 1,2-dioxygenase, at 774,475 to 775,524
_RS24280
Position (kb) 772
773
774 Strain fitness (log2 ratio) -2
-1
0
1
2 at 771.526 kb on - strand at 771.526 kb on - strand at 771.613 kb on - strand at 771.701 kb on - strand at 771.742 kb on + strand at 771.743 kb on - strand at 771.743 kb on - strand at 771.758 kb on + strand at 771.758 kb on + strand at 771.787 kb on - strand, within RR42_RS24265 at 771.896 kb on + strand, within RR42_RS24265 at 771.955 kb on + strand, within RR42_RS24265 at 771.959 kb on - strand, within RR42_RS24265 at 771.984 kb on + strand, within RR42_RS24265 at 771.985 kb on - strand, within RR42_RS24265 at 771.985 kb on - strand, within RR42_RS24265 at 772.035 kb on + strand, within RR42_RS24265 at 772.036 kb on - strand, within RR42_RS24265 at 772.036 kb on - strand, within RR42_RS24265 at 772.198 kb on + strand, within RR42_RS24265 at 772.198 kb on + strand, within RR42_RS24265 at 772.198 kb on + strand, within RR42_RS24265 at 772.199 kb on - strand, within RR42_RS24265 at 772.199 kb on - strand, within RR42_RS24265 at 772.199 kb on - strand, within RR42_RS24265 at 772.199 kb on - strand, within RR42_RS24265 at 772.199 kb on - strand, within RR42_RS24265 at 772.199 kb on - strand, within RR42_RS24265 at 772.459 kb on + strand at 772.460 kb on - strand at 772.460 kb on - strand at 772.463 kb on + strand at 772.463 kb on + strand at 772.464 kb on - strand at 772.526 kb on + strand at 772.527 kb on - strand at 772.580 kb on - strand at 772.580 kb on - strand at 772.580 kb on - strand at 772.580 kb on - strand at 772.755 kb on + strand, within RR42_RS24270 at 772.853 kb on - strand, within RR42_RS24270 at 772.938 kb on + strand, within RR42_RS24270 at 772.938 kb on + strand, within RR42_RS24270 at 772.939 kb on - strand at 773.164 kb on - strand, within RR42_RS24270 at 773.164 kb on - strand, within RR42_RS24270 at 773.164 kb on - strand, within RR42_RS24270 at 773.230 kb on - strand, within RR42_RS24270 at 773.469 kb on - strand at 773.504 kb on - strand at 773.592 kb on + strand at 773.593 kb on - strand at 773.595 kb on + strand at 773.595 kb on + strand at 773.595 kb on + strand at 773.596 kb on - strand at 773.675 kb on - strand at 773.834 kb on + strand, within RR42_RS24275 at 773.834 kb on + strand, within RR42_RS24275 at 773.834 kb on + strand, within RR42_RS24275 at 773.835 kb on - strand, within RR42_RS24275 at 774.000 kb on + strand, within RR42_RS24275 at 774.000 kb on + strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.243 kb on - strand, within RR42_RS24275 at 774.401 kb on + strand at 774.402 kb on - strand at 774.420 kb on + strand at 774.420 kb on + strand at 774.420 kb on + strand at 774.421 kb on - strand at 774.421 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 771,526 - +1.6 771,526 - +0.1 771,613 - -1.1 771,701 - -0.5 771,742 + +1.0 771,743 - +0.9 771,743 - +0.2 771,758 + +0.2 771,758 + +0.3 771,787 - RR42_RS24265 0.12 -0.1 771,896 + RR42_RS24265 0.27 +1.0 771,955 + RR42_RS24265 0.36 -1.0 771,959 - RR42_RS24265 0.36 +1.0 771,984 + RR42_RS24265 0.40 +0.0 771,985 - RR42_RS24265 0.40 +0.2 771,985 - RR42_RS24265 0.40 +0.9 772,035 + RR42_RS24265 0.47 +1.1 772,036 - RR42_RS24265 0.47 -1.0 772,036 - RR42_RS24265 0.47 +0.6 772,198 + RR42_RS24265 0.70 -0.4 772,198 + RR42_RS24265 0.70 +0.6 772,198 + RR42_RS24265 0.70 -0.8 772,199 - RR42_RS24265 0.70 +1.0 772,199 - RR42_RS24265 0.70 +0.4 772,199 - RR42_RS24265 0.70 +0.2 772,199 - RR42_RS24265 0.70 -0.1 772,199 - RR42_RS24265 0.70 +0.2 772,199 - RR42_RS24265 0.70 +0.5 772,459 + +0.2 772,460 - +0.5 772,460 - +0.2 772,463 + -0.9 772,463 + +0.4 772,464 - +0.3 772,526 + +0.3 772,527 - -0.6 772,580 - +0.4 772,580 - -0.7 772,580 - +0.1 772,580 - -0.8 772,755 + RR42_RS24270 0.25 +0.5 772,853 - RR42_RS24270 0.35 +1.6 772,938 + RR42_RS24270 0.44 +0.2 772,938 + RR42_RS24270 0.44 +0.7 772,939 - +1.8 773,164 - RR42_RS24270 0.67 +0.1 773,164 - RR42_RS24270 0.67 +0.8 773,164 - RR42_RS24270 0.67 +0.5 773,230 - RR42_RS24270 0.74 -0.4 773,469 - -0.8 773,504 - +0.9 773,592 + -0.0 773,593 - -0.5 773,595 + +0.4 773,595 + -0.3 773,595 + +0.4 773,596 - -0.3 773,675 - -0.9 773,834 + RR42_RS24275 0.27 -0.1 773,834 + RR42_RS24275 0.27 -0.9 773,834 + RR42_RS24275 0.27 -0.4 773,835 - RR42_RS24275 0.27 -0.2 774,000 + RR42_RS24275 0.46 +0.5 774,000 + RR42_RS24275 0.46 -0.2 774,243 - RR42_RS24275 0.73 -0.9 774,243 - RR42_RS24275 0.73 -0.4 774,243 - RR42_RS24275 0.73 +0.7 774,243 - RR42_RS24275 0.73 +0.3 774,401 + +0.8 774,402 - +0.8 774,420 + -1.7 774,420 + -2.2 774,420 + +0.4 774,421 - +0.1 774,421 - -1.6
Or see this region's nucleotide sequence