Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24090 and RR42_RS24095 are separated by 76 nucleotides RR42_RS24095 and RR42_RS24100 are separated by 115 nucleotides RR42_RS24100 and RR42_RS24105 are separated by 384 nucleotides
RR42_RS24090: RR42_RS24090 - quinone oxidoreductase, at 729,487 to 730,479
_RS24090
RR42_RS24095: RR42_RS24095 - hypothetical protein, at 730,556 to 730,885
_RS24095
RR42_RS24100: RR42_RS24100 - hypothetical protein, at 731,001 to 731,516
_RS24100
RR42_RS24105: RR42_RS24105 - membrane protein, at 731,901 to 733,004
_RS24105
Position (kb)
731
732 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 730.026 kb on + strand, within RR42_RS24090 at 730.027 kb on - strand, within RR42_RS24090 at 730.027 kb on - strand, within RR42_RS24090 at 730.027 kb on - strand, within RR42_RS24090 at 730.200 kb on + strand, within RR42_RS24090 at 730.200 kb on + strand, within RR42_RS24090 at 730.200 kb on + strand, within RR42_RS24090 at 730.210 kb on + strand, within RR42_RS24090 at 730.210 kb on + strand, within RR42_RS24090 at 730.230 kb on + strand, within RR42_RS24090 at 730.231 kb on - strand, within RR42_RS24090 at 730.231 kb on - strand, within RR42_RS24090 at 730.231 kb on - strand, within RR42_RS24090 at 730.326 kb on - strand, within RR42_RS24090 at 730.326 kb on - strand, within RR42_RS24090 at 730.396 kb on + strand at 730.396 kb on + strand at 730.396 kb on + strand at 730.451 kb on + strand at 730.452 kb on - strand at 730.452 kb on - strand at 730.456 kb on - strand at 730.496 kb on + strand at 730.497 kb on - strand at 730.497 kb on - strand at 730.640 kb on + strand, within RR42_RS24095 at 730.640 kb on + strand, within RR42_RS24095 at 730.640 kb on + strand, within RR42_RS24095 at 730.641 kb on - strand, within RR42_RS24095 at 730.641 kb on - strand, within RR42_RS24095 at 730.654 kb on - strand, within RR42_RS24095 at 730.673 kb on + strand, within RR42_RS24095 at 730.673 kb on + strand, within RR42_RS24095 at 730.673 kb on + strand, within RR42_RS24095 at 730.673 kb on + strand, within RR42_RS24095 at 730.674 kb on - strand, within RR42_RS24095 at 730.734 kb on + strand, within RR42_RS24095 at 730.735 kb on - strand, within RR42_RS24095 at 730.807 kb on + strand, within RR42_RS24095 at 730.807 kb on + strand, within RR42_RS24095 at 730.807 kb on + strand, within RR42_RS24095 at 730.895 kb on - strand at 730.906 kb on - strand at 730.906 kb on - strand at 731.137 kb on + strand, within RR42_RS24100 at 731.220 kb on + strand, within RR42_RS24100 at 731.232 kb on + strand, within RR42_RS24100 at 731.232 kb on + strand, within RR42_RS24100 at 731.233 kb on - strand, within RR42_RS24100 at 731.233 kb on - strand, within RR42_RS24100 at 731.330 kb on + strand, within RR42_RS24100 at 731.331 kb on - strand, within RR42_RS24100 at 731.352 kb on + strand, within RR42_RS24100 at 731.352 kb on + strand, within RR42_RS24100 at 731.352 kb on + strand, within RR42_RS24100 at 731.458 kb on + strand, within RR42_RS24100 at 731.512 kb on + strand at 731.512 kb on + strand at 731.513 kb on - strand at 731.513 kb on - strand at 731.513 kb on - strand at 731.544 kb on - strand at 731.544 kb on - strand at 731.552 kb on + strand at 731.553 kb on - strand at 731.553 kb on - strand at 731.568 kb on + strand at 731.568 kb on + strand at 731.569 kb on - strand at 731.630 kb on - strand at 731.647 kb on - strand at 731.647 kb on - strand at 731.647 kb on - strand at 731.647 kb on - strand at 731.895 kb on + strand at 731.895 kb on + strand at 731.895 kb on + strand at 731.896 kb on - strand at 731.896 kb on - strand at 731.896 kb on - strand at 731.902 kb on + strand at 731.903 kb on - strand at 731.903 kb on - strand at 732.017 kb on - strand, within RR42_RS24105 at 732.017 kb on - strand, within RR42_RS24105 at 732.058 kb on + strand, within RR42_RS24105 at 732.058 kb on + strand, within RR42_RS24105 at 732.058 kb on + strand, within RR42_RS24105 at 732.059 kb on - strand, within RR42_RS24105 at 732.059 kb on - strand, within RR42_RS24105 at 732.059 kb on - strand, within RR42_RS24105 at 732.059 kb on - strand, within RR42_RS24105 at 732.059 kb on - strand, within RR42_RS24105 at 732.059 kb on - strand, within RR42_RS24105 at 732.059 kb on - strand, within RR42_RS24105 at 732.137 kb on + strand, within RR42_RS24105 at 732.152 kb on + strand, within RR42_RS24105 at 732.152 kb on + strand, within RR42_RS24105 at 732.173 kb on + strand, within RR42_RS24105 at 732.173 kb on - strand, within RR42_RS24105 at 732.173 kb on - strand, within RR42_RS24105 at 732.175 kb on + strand, within RR42_RS24105 at 732.176 kb on - strand, within RR42_RS24105 at 732.259 kb on + strand, within RR42_RS24105 at 732.288 kb on + strand, within RR42_RS24105 at 732.343 kb on + strand, within RR42_RS24105 at 732.344 kb on - strand, within RR42_RS24105 at 732.356 kb on + strand, within RR42_RS24105 at 732.371 kb on + strand, within RR42_RS24105 at 732.371 kb on + strand, within RR42_RS24105 at 732.372 kb on - strand, within RR42_RS24105 at 732.372 kb on - strand, within RR42_RS24105 at 732.376 kb on + strand, within RR42_RS24105 at 732.377 kb on - strand, within RR42_RS24105 at 732.384 kb on + strand, within RR42_RS24105 at 732.384 kb on + strand, within RR42_RS24105 at 732.385 kb on - strand, within RR42_RS24105 at 732.388 kb on - strand, within RR42_RS24105 at 732.490 kb on + strand, within RR42_RS24105 at 732.490 kb on + strand, within RR42_RS24105 at 732.491 kb on - strand, within RR42_RS24105 at 732.491 kb on - strand, within RR42_RS24105 at 732.491 kb on - strand, within RR42_RS24105 at 732.491 kb on - strand, within RR42_RS24105 at 732.494 kb on + strand, within RR42_RS24105
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 730,026 + RR42_RS24090 0.54 -0.1 730,027 - RR42_RS24090 0.54 +0.3 730,027 - RR42_RS24090 0.54 +0.2 730,027 - RR42_RS24090 0.54 +0.0 730,200 + RR42_RS24090 0.72 -0.1 730,200 + RR42_RS24090 0.72 -1.4 730,200 + RR42_RS24090 0.72 +0.4 730,210 + RR42_RS24090 0.73 +0.4 730,210 + RR42_RS24090 0.73 +0.1 730,230 + RR42_RS24090 0.75 -0.4 730,231 - RR42_RS24090 0.75 +1.3 730,231 - RR42_RS24090 0.75 +0.1 730,231 - RR42_RS24090 0.75 -0.5 730,326 - RR42_RS24090 0.84 +0.1 730,326 - RR42_RS24090 0.84 -0.5 730,396 + +2.6 730,396 + +0.7 730,396 + +0.5 730,451 + -0.1 730,452 - -0.9 730,452 - -0.4 730,456 - +1.6 730,496 + +0.2 730,497 - +0.7 730,497 - -0.1 730,640 + RR42_RS24095 0.25 +0.3 730,640 + RR42_RS24095 0.25 +0.4 730,640 + RR42_RS24095 0.25 -0.0 730,641 - RR42_RS24095 0.26 -0.8 730,641 - RR42_RS24095 0.26 -0.1 730,654 - RR42_RS24095 0.30 +0.7 730,673 + RR42_RS24095 0.35 +0.5 730,673 + RR42_RS24095 0.35 -0.3 730,673 + RR42_RS24095 0.35 -0.3 730,673 + RR42_RS24095 0.35 +0.6 730,674 - RR42_RS24095 0.36 -0.2 730,734 + RR42_RS24095 0.54 -3.5 730,735 - RR42_RS24095 0.54 +0.9 730,807 + RR42_RS24095 0.76 +0.2 730,807 + RR42_RS24095 0.76 +0.4 730,807 + RR42_RS24095 0.76 +0.6 730,895 - -0.2 730,906 - +0.5 730,906 - -0.8 731,137 + RR42_RS24100 0.26 +0.1 731,220 + RR42_RS24100 0.42 -0.2 731,232 + RR42_RS24100 0.45 +0.4 731,232 + RR42_RS24100 0.45 +0.3 731,233 - RR42_RS24100 0.45 +0.7 731,233 - RR42_RS24100 0.45 -0.1 731,330 + RR42_RS24100 0.64 -0.1 731,331 - RR42_RS24100 0.64 -0.3 731,352 + RR42_RS24100 0.68 +0.7 731,352 + RR42_RS24100 0.68 +0.4 731,352 + RR42_RS24100 0.68 -0.1 731,458 + RR42_RS24100 0.89 -0.6 731,512 + +0.6 731,512 + +0.5 731,513 - +0.4 731,513 - -0.5 731,513 - -0.2 731,544 - -3.0 731,544 - +0.0 731,552 + +0.8 731,553 - +1.8 731,553 - -0.5 731,568 + +0.4 731,568 + +0.3 731,569 - -0.6 731,630 - -1.2 731,647 - +1.2 731,647 - -1.5 731,647 - -1.4 731,647 - -0.2 731,895 + +0.4 731,895 + -0.6 731,895 + +0.2 731,896 - +0.0 731,896 - +0.6 731,896 - -2.2 731,902 + +0.0 731,903 - -0.2 731,903 - -0.0 732,017 - RR42_RS24105 0.11 -0.6 732,017 - RR42_RS24105 0.11 -0.1 732,058 + RR42_RS24105 0.14 +1.4 732,058 + RR42_RS24105 0.14 +0.9 732,058 + RR42_RS24105 0.14 +0.0 732,059 - RR42_RS24105 0.14 -0.2 732,059 - RR42_RS24105 0.14 +0.6 732,059 - RR42_RS24105 0.14 -0.0 732,059 - RR42_RS24105 0.14 -2.4 732,059 - RR42_RS24105 0.14 -1.2 732,059 - RR42_RS24105 0.14 -0.5 732,059 - RR42_RS24105 0.14 +0.8 732,137 + RR42_RS24105 0.21 +0.4 732,152 + RR42_RS24105 0.23 -0.1 732,152 + RR42_RS24105 0.23 +0.6 732,173 + RR42_RS24105 0.25 +0.6 732,173 - RR42_RS24105 0.25 -1.3 732,173 - RR42_RS24105 0.25 -0.5 732,175 + RR42_RS24105 0.25 -1.1 732,176 - RR42_RS24105 0.25 -0.4 732,259 + RR42_RS24105 0.32 -0.1 732,288 + RR42_RS24105 0.35 +1.2 732,343 + RR42_RS24105 0.40 -0.2 732,344 - RR42_RS24105 0.40 +0.0 732,356 + RR42_RS24105 0.41 +0.1 732,371 + RR42_RS24105 0.43 +2.2 732,371 + RR42_RS24105 0.43 +0.4 732,372 - RR42_RS24105 0.43 +0.7 732,372 - RR42_RS24105 0.43 +0.3 732,376 + RR42_RS24105 0.43 +0.1 732,377 - RR42_RS24105 0.43 +1.4 732,384 + RR42_RS24105 0.44 +0.2 732,384 + RR42_RS24105 0.44 +0.2 732,385 - RR42_RS24105 0.44 +0.1 732,388 - RR42_RS24105 0.44 -0.4 732,490 + RR42_RS24105 0.53 +0.7 732,490 + RR42_RS24105 0.53 -0.0 732,491 - RR42_RS24105 0.53 +0.1 732,491 - RR42_RS24105 0.53 +0.2 732,491 - RR42_RS24105 0.53 -0.6 732,491 - RR42_RS24105 0.53 +0.8 732,494 + RR42_RS24105 0.54 +1.4
Or see this region's nucleotide sequence