Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23795 and RR42_RS23800 are separated by 331 nucleotides RR42_RS23800 and RR42_RS23805 are separated by 407 nucleotides
RR42_RS23795: RR42_RS23795 - hypothetical protein, at 662,441 to 666,097
_RS23795
RR42_RS23800: RR42_RS23800 - hypothetical protein, at 666,429 to 666,728
_RS23800
RR42_RS23805: RR42_RS23805 - hypothetical protein, at 667,136 to 669,109
_RS23805
Position (kb)
666
667 Strain fitness (log2 ratio)
-2
-1
0
1 at 665.469 kb on + strand, within RR42_RS23795 at 665.469 kb on + strand, within RR42_RS23795 at 665.470 kb on - strand, within RR42_RS23795 at 665.470 kb on - strand, within RR42_RS23795 at 665.548 kb on - strand, within RR42_RS23795 at 665.637 kb on + strand, within RR42_RS23795 at 665.637 kb on + strand, within RR42_RS23795 at 665.660 kb on + strand, within RR42_RS23795 at 665.661 kb on - strand, within RR42_RS23795 at 665.661 kb on - strand, within RR42_RS23795 at 665.715 kb on + strand, within RR42_RS23795 at 665.739 kb on + strand at 665.740 kb on - strand at 665.813 kb on + strand at 665.813 kb on + strand at 665.813 kb on + strand at 665.814 kb on - strand at 665.871 kb on + strand at 665.871 kb on + strand at 665.910 kb on - strand at 665.910 kb on - strand at 666.106 kb on + strand at 666.117 kb on + strand at 666.117 kb on + strand at 666.118 kb on - strand at 666.131 kb on + strand at 666.151 kb on + strand at 666.152 kb on - strand at 666.152 kb on - strand at 666.169 kb on + strand at 666.177 kb on - strand at 666.208 kb on + strand at 666.282 kb on - strand at 666.282 kb on - strand at 666.282 kb on - strand at 666.282 kb on - strand at 666.340 kb on + strand at 666.340 kb on + strand at 666.340 kb on + strand at 666.360 kb on + strand at 666.361 kb on - strand at 666.376 kb on + strand at 666.377 kb on - strand at 666.436 kb on + strand at 666.437 kb on - strand at 666.459 kb on + strand, within RR42_RS23800 at 666.459 kb on + strand, within RR42_RS23800 at 666.460 kb on - strand, within RR42_RS23800 at 666.460 kb on - strand, within RR42_RS23800 at 666.491 kb on + strand, within RR42_RS23800 at 666.524 kb on - strand, within RR42_RS23800 at 666.524 kb on - strand, within RR42_RS23800 at 666.524 kb on - strand, within RR42_RS23800 at 666.528 kb on + strand, within RR42_RS23800 at 666.529 kb on - strand, within RR42_RS23800 at 666.529 kb on - strand, within RR42_RS23800 at 666.529 kb on - strand, within RR42_RS23800 at 666.577 kb on + strand, within RR42_RS23800 at 666.577 kb on + strand, within RR42_RS23800 at 666.577 kb on + strand, within RR42_RS23800 at 666.578 kb on - strand, within RR42_RS23800 at 666.592 kb on + strand, within RR42_RS23800 at 666.593 kb on - strand, within RR42_RS23800 at 666.685 kb on - strand, within RR42_RS23800 at 666.706 kb on - strand at 666.706 kb on - strand at 666.878 kb on - strand at 666.901 kb on - strand at 666.962 kb on - strand at 666.962 kb on - strand at 666.970 kb on + strand at 666.971 kb on - strand at 667.000 kb on + strand at 667.000 kb on + strand at 667.001 kb on - strand at 667.041 kb on + strand at 667.041 kb on + strand at 667.042 kb on - strand at 667.042 kb on - strand at 667.042 kb on - strand at 667.051 kb on + strand at 667.051 kb on + strand at 667.051 kb on + strand at 667.052 kb on - strand at 667.052 kb on - strand at 667.052 kb on - strand at 667.057 kb on + strand at 667.057 kb on + strand at 667.057 kb on + strand at 667.058 kb on - strand at 667.058 kb on - strand at 667.058 kb on - strand at 667.058 kb on - strand at 667.058 kb on - strand at 667.288 kb on - strand at 667.288 kb on - strand at 667.288 kb on - strand at 667.513 kb on + strand, within RR42_RS23805 at 667.513 kb on + strand, within RR42_RS23805 at 667.659 kb on - strand, within RR42_RS23805
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 665,469 + RR42_RS23795 0.83 +0.5 665,469 + RR42_RS23795 0.83 +0.4 665,470 - RR42_RS23795 0.83 +1.0 665,470 - RR42_RS23795 0.83 +0.7 665,548 - RR42_RS23795 0.85 +0.4 665,637 + RR42_RS23795 0.87 +0.4 665,637 + RR42_RS23795 0.87 +0.5 665,660 + RR42_RS23795 0.88 -1.0 665,661 - RR42_RS23795 0.88 +0.1 665,661 - RR42_RS23795 0.88 +0.6 665,715 + RR42_RS23795 0.90 +0.1 665,739 + +0.1 665,740 - +0.3 665,813 + +0.2 665,813 + +1.2 665,813 + -0.4 665,814 - +0.4 665,871 + +0.1 665,871 + -0.4 665,910 - -0.0 665,910 - +1.5 666,106 + +0.1 666,117 + -2.3 666,117 + +0.6 666,118 - -0.3 666,131 + +0.8 666,151 + -1.3 666,152 - +0.6 666,152 - +0.3 666,169 + -0.4 666,177 - +0.5 666,208 + +0.7 666,282 - -1.2 666,282 - -0.0 666,282 - -1.4 666,282 - +0.4 666,340 + +1.1 666,340 + -0.3 666,340 + +1.4 666,360 + +0.4 666,361 - -0.7 666,376 + -0.8 666,377 - +0.6 666,436 + -1.0 666,437 - -0.0 666,459 + RR42_RS23800 0.10 -0.2 666,459 + RR42_RS23800 0.10 +0.2 666,460 - RR42_RS23800 0.10 -0.1 666,460 - RR42_RS23800 0.10 -0.4 666,491 + RR42_RS23800 0.21 -0.5 666,524 - RR42_RS23800 0.32 +0.4 666,524 - RR42_RS23800 0.32 +1.0 666,524 - RR42_RS23800 0.32 -0.5 666,528 + RR42_RS23800 0.33 -0.2 666,529 - RR42_RS23800 0.33 +0.5 666,529 - RR42_RS23800 0.33 -0.2 666,529 - RR42_RS23800 0.33 +0.2 666,577 + RR42_RS23800 0.49 -0.4 666,577 + RR42_RS23800 0.49 +0.6 666,577 + RR42_RS23800 0.49 +1.5 666,578 - RR42_RS23800 0.50 +0.2 666,592 + RR42_RS23800 0.54 +0.7 666,593 - RR42_RS23800 0.55 +0.2 666,685 - RR42_RS23800 0.85 -0.2 666,706 - +1.2 666,706 - +0.8 666,878 - +1.3 666,901 - +0.8 666,962 - -0.1 666,962 - +0.1 666,970 + +0.0 666,971 - +0.5 667,000 + -0.6 667,000 + -0.5 667,001 - -0.8 667,041 + +1.0 667,041 + -0.1 667,042 - -2.0 667,042 - +0.3 667,042 - -0.9 667,051 + -1.5 667,051 + -0.2 667,051 + -0.0 667,052 - +0.2 667,052 - +0.9 667,052 - +1.1 667,057 + +1.4 667,057 + +1.1 667,057 + +1.2 667,058 - -0.1 667,058 - +0.9 667,058 - +0.4 667,058 - +0.5 667,058 - +1.6 667,288 - -0.1 667,288 - -0.2 667,288 - +0.3 667,513 + RR42_RS23805 0.19 +0.2 667,513 + RR42_RS23805 0.19 +1.1 667,659 - RR42_RS23805 0.26 -1.4
Or see this region's nucleotide sequence