Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23780 and RR42_RS23785 are separated by 305 nucleotides RR42_RS23785 and RR42_RS23790 are separated by 385 nucleotides
RR42_RS23780: RR42_RS23780 - hypothetical protein, at 658,815 to 659,753
_RS23780
RR42_RS23785: RR42_RS23785 - hypothetical protein, at 660,059 to 660,319
_RS23785
RR42_RS23790: RR42_RS23790 - hypothetical protein, at 660,705 to 662,045
_RS23790
Position (kb)
660
661 Strain fitness (log2 ratio)
-2
-1
0
1 at 659.117 kb on - strand, within RR42_RS23780 at 659.124 kb on + strand, within RR42_RS23780 at 659.125 kb on - strand, within RR42_RS23780 at 659.129 kb on + strand, within RR42_RS23780 at 659.129 kb on + strand, within RR42_RS23780 at 659.129 kb on + strand, within RR42_RS23780 at 659.130 kb on - strand, within RR42_RS23780 at 659.263 kb on + strand, within RR42_RS23780 at 659.304 kb on + strand, within RR42_RS23780 at 659.304 kb on + strand, within RR42_RS23780 at 659.305 kb on - strand, within RR42_RS23780 at 659.306 kb on + strand, within RR42_RS23780 at 659.307 kb on - strand, within RR42_RS23780 at 659.558 kb on - strand, within RR42_RS23780 at 659.656 kb on - strand, within RR42_RS23780 at 659.656 kb on - strand, within RR42_RS23780 at 659.656 kb on - strand, within RR42_RS23780 at 659.656 kb on - strand, within RR42_RS23780 at 659.656 kb on - strand, within RR42_RS23780 at 659.656 kb on - strand, within RR42_RS23780 at 659.656 kb on - strand, within RR42_RS23780 at 659.819 kb on + strand at 659.819 kb on + strand at 659.819 kb on + strand at 659.903 kb on - strand at 659.903 kb on - strand at 659.903 kb on - strand at 659.934 kb on + strand at 660.068 kb on - strand at 660.075 kb on - strand at 660.095 kb on + strand, within RR42_RS23785 at 660.190 kb on + strand, within RR42_RS23785 at 660.190 kb on + strand, within RR42_RS23785 at 660.191 kb on - strand, within RR42_RS23785 at 660.191 kb on - strand, within RR42_RS23785 at 660.191 kb on - strand, within RR42_RS23785 at 660.193 kb on - strand, within RR42_RS23785 at 660.215 kb on + strand, within RR42_RS23785 at 660.215 kb on + strand, within RR42_RS23785 at 660.216 kb on - strand, within RR42_RS23785 at 660.216 kb on - strand, within RR42_RS23785 at 660.317 kb on + strand at 660.320 kb on + strand at 660.321 kb on - strand at 660.329 kb on - strand at 660.330 kb on + strand at 660.330 kb on + strand at 660.331 kb on - strand at 660.331 kb on - strand at 660.380 kb on + strand at 660.381 kb on - strand at 660.381 kb on - strand at 660.426 kb on + strand at 660.426 kb on + strand at 660.533 kb on + strand at 660.534 kb on - strand at 660.534 kb on - strand at 660.536 kb on + strand at 660.536 kb on + strand at 660.536 kb on + strand at 660.537 kb on - strand at 660.547 kb on - strand at 660.589 kb on - strand at 660.589 kb on - strand at 660.678 kb on + strand at 660.678 kb on + strand at 660.678 kb on + strand at 660.678 kb on + strand at 660.679 kb on - strand at 660.777 kb on + strand at 660.778 kb on - strand at 660.801 kb on + strand at 660.801 kb on + strand at 660.801 kb on + strand at 660.801 kb on + strand at 660.801 kb on + strand at 660.802 kb on - strand at 660.802 kb on - strand at 660.802 kb on - strand at 660.802 kb on - strand at 660.804 kb on - strand at 660.832 kb on + strand at 660.832 kb on + strand at 660.832 kb on + strand at 660.833 kb on - strand at 660.833 kb on - strand at 660.833 kb on - strand at 660.907 kb on - strand, within RR42_RS23790 at 661.007 kb on - strand, within RR42_RS23790 at 661.074 kb on + strand, within RR42_RS23790 at 661.160 kb on + strand, within RR42_RS23790 at 661.239 kb on + strand, within RR42_RS23790
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 659,117 - RR42_RS23780 0.32 -0.1 659,124 + RR42_RS23780 0.33 -0.1 659,125 - RR42_RS23780 0.33 -2.0 659,129 + RR42_RS23780 0.33 -1.7 659,129 + RR42_RS23780 0.33 +1.3 659,129 + RR42_RS23780 0.33 -0.9 659,130 - RR42_RS23780 0.34 -0.1 659,263 + RR42_RS23780 0.48 -0.9 659,304 + RR42_RS23780 0.52 -0.9 659,304 + RR42_RS23780 0.52 +0.1 659,305 - RR42_RS23780 0.52 +0.1 659,306 + RR42_RS23780 0.52 -0.6 659,307 - RR42_RS23780 0.52 -0.6 659,558 - RR42_RS23780 0.79 -0.8 659,656 - RR42_RS23780 0.90 -0.1 659,656 - RR42_RS23780 0.90 -0.1 659,656 - RR42_RS23780 0.90 -1.0 659,656 - RR42_RS23780 0.90 +0.7 659,656 - RR42_RS23780 0.90 -0.2 659,656 - RR42_RS23780 0.90 +0.3 659,656 - RR42_RS23780 0.90 +0.6 659,819 + -0.2 659,819 + -0.4 659,819 + -0.0 659,903 - -0.9 659,903 - -0.1 659,903 - -1.9 659,934 + -0.1 660,068 - -0.2 660,075 - -1.0 660,095 + RR42_RS23785 0.14 -0.1 660,190 + RR42_RS23785 0.50 -0.3 660,190 + RR42_RS23785 0.50 +1.1 660,191 - RR42_RS23785 0.51 -0.3 660,191 - RR42_RS23785 0.51 -0.1 660,191 - RR42_RS23785 0.51 +0.2 660,193 - RR42_RS23785 0.51 -2.5 660,215 + RR42_RS23785 0.60 -0.3 660,215 + RR42_RS23785 0.60 +0.1 660,216 - RR42_RS23785 0.60 -0.0 660,216 - RR42_RS23785 0.60 -0.5 660,317 + +0.9 660,320 + +0.4 660,321 - +1.7 660,329 - -0.6 660,330 + +1.0 660,330 + +0.1 660,331 - +0.3 660,331 - -0.5 660,380 + +0.4 660,381 - +0.1 660,381 - -0.3 660,426 + +0.5 660,426 + +0.7 660,533 + -0.3 660,534 - +1.2 660,534 - +0.5 660,536 + -0.3 660,536 + +0.5 660,536 + +0.6 660,537 - +0.1 660,547 - -0.3 660,589 - -0.1 660,589 - +0.2 660,678 + +1.0 660,678 + +1.4 660,678 + +0.0 660,678 + +0.2 660,679 - +0.3 660,777 + -0.5 660,778 - +0.3 660,801 + -0.6 660,801 + -0.7 660,801 + +0.1 660,801 + -0.2 660,801 + +0.4 660,802 - +0.4 660,802 - +0.0 660,802 - +0.2 660,802 - +0.7 660,804 - -0.5 660,832 + +0.3 660,832 + +0.5 660,832 + -0.1 660,833 - +0.4 660,833 - +0.1 660,833 - +0.6 660,907 - RR42_RS23790 0.15 -1.4 661,007 - RR42_RS23790 0.23 +0.1 661,074 + RR42_RS23790 0.28 -1.1 661,160 + RR42_RS23790 0.34 +0.4 661,239 + RR42_RS23790 0.40 +0.2
Or see this region's nucleotide sequence