Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23755 and RR42_RS23760 are separated by 140 nucleotides RR42_RS23760 and RR42_RS23765 are separated by 4 nucleotides RR42_RS23765 and RR42_RS23770 are separated by 104 nucleotides
RR42_RS23755: RR42_RS23755 - phenylacetate--CoA ligase, at 654,283 to 655,608
_RS23755
RR42_RS23760: RR42_RS23760 - tRNA-Asn, at 655,749 to 655,824
_RS23760
RR42_RS23765: RR42_RS23765 - tRNA-Gly, at 655,829 to 655,904
_RS23765
RR42_RS23770: RR42_RS23770 - hypothetical protein, at 656,009 to 656,920
_RS23770
Position (kb) 655
656 Strain fitness (log2 ratio) -2
-1
0
1
2 at 654.803 kb on + strand, within RR42_RS23755 at 654.804 kb on - strand, within RR42_RS23755 at 654.804 kb on - strand, within RR42_RS23755 at 654.804 kb on - strand, within RR42_RS23755 at 654.853 kb on + strand, within RR42_RS23755 at 654.853 kb on + strand, within RR42_RS23755 at 654.853 kb on + strand, within RR42_RS23755 at 654.853 kb on + strand, within RR42_RS23755 at 654.854 kb on - strand, within RR42_RS23755 at 654.854 kb on - strand, within RR42_RS23755 at 654.861 kb on + strand, within RR42_RS23755 at 654.862 kb on - strand, within RR42_RS23755 at 655.003 kb on + strand, within RR42_RS23755 at 655.004 kb on - strand, within RR42_RS23755 at 655.004 kb on - strand, within RR42_RS23755 at 655.004 kb on - strand, within RR42_RS23755 at 655.158 kb on + strand, within RR42_RS23755 at 655.159 kb on - strand, within RR42_RS23755 at 655.363 kb on + strand, within RR42_RS23755 at 655.363 kb on + strand, within RR42_RS23755 at 655.364 kb on - strand, within RR42_RS23755 at 655.392 kb on + strand, within RR42_RS23755 at 655.756 kb on + strand at 655.768 kb on + strand, within RR42_RS23760 at 655.784 kb on + strand, within RR42_RS23760 at 655.784 kb on + strand, within RR42_RS23760 at 655.837 kb on - strand, within RR42_RS23765 at 655.837 kb on - strand, within RR42_RS23765 at 655.849 kb on - strand, within RR42_RS23765 at 655.849 kb on - strand, within RR42_RS23765 at 655.925 kb on + strand at 655.925 kb on + strand at 655.926 kb on - strand at 655.931 kb on - strand at 656.010 kb on + strand at 656.011 kb on - strand at 656.017 kb on - strand at 656.017 kb on - strand at 656.058 kb on + strand at 656.058 kb on + strand at 656.058 kb on + strand at 656.058 kb on + strand at 656.058 kb on + strand at 656.071 kb on + strand at 656.083 kb on + strand at 656.083 kb on + strand at 656.083 kb on + strand at 656.083 kb on + strand at 656.084 kb on - strand at 656.084 kb on - strand at 656.149 kb on + strand, within RR42_RS23770 at 656.149 kb on + strand, within RR42_RS23770 at 656.177 kb on + strand, within RR42_RS23770 at 656.177 kb on + strand, within RR42_RS23770 at 656.178 kb on - strand, within RR42_RS23770 at 656.178 kb on - strand, within RR42_RS23770 at 656.178 kb on - strand, within RR42_RS23770 at 656.188 kb on + strand, within RR42_RS23770 at 656.189 kb on - strand, within RR42_RS23770 at 656.189 kb on - strand, within RR42_RS23770 at 656.189 kb on - strand, within RR42_RS23770 at 656.328 kb on + strand, within RR42_RS23770 at 656.330 kb on + strand, within RR42_RS23770 at 656.331 kb on - strand, within RR42_RS23770 at 656.341 kb on - strand, within RR42_RS23770 at 656.425 kb on + strand, within RR42_RS23770 at 656.425 kb on + strand, within RR42_RS23770 at 656.426 kb on - strand, within RR42_RS23770 at 656.426 kb on - strand, within RR42_RS23770 at 656.426 kb on - strand, within RR42_RS23770 at 656.444 kb on + strand, within RR42_RS23770 at 656.444 kb on + strand, within RR42_RS23770 at 656.445 kb on - strand, within RR42_RS23770 at 656.497 kb on - strand, within RR42_RS23770 at 656.530 kb on + strand, within RR42_RS23770 at 656.531 kb on - strand, within RR42_RS23770 at 656.531 kb on - strand, within RR42_RS23770 at 656.531 kb on - strand, within RR42_RS23770 at 656.573 kb on + strand, within RR42_RS23770 at 656.574 kb on - strand, within RR42_RS23770 at 656.583 kb on - strand, within RR42_RS23770 at 656.720 kb on + strand, within RR42_RS23770 at 656.726 kb on - strand, within RR42_RS23770 at 656.730 kb on + strand, within RR42_RS23770 at 656.730 kb on + strand, within RR42_RS23770 at 656.731 kb on - strand, within RR42_RS23770 at 656.731 kb on - strand, within RR42_RS23770 at 656.731 kb on - strand, within RR42_RS23770 at 656.777 kb on - strand, within RR42_RS23770 at 656.810 kb on - strand, within RR42_RS23770
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 654,803 + RR42_RS23755 0.39 +0.4 654,804 - RR42_RS23755 0.39 -0.3 654,804 - RR42_RS23755 0.39 -0.4 654,804 - RR42_RS23755 0.39 -0.4 654,853 + RR42_RS23755 0.43 -1.0 654,853 + RR42_RS23755 0.43 -0.1 654,853 + RR42_RS23755 0.43 -0.1 654,853 + RR42_RS23755 0.43 -0.0 654,854 - RR42_RS23755 0.43 -1.0 654,854 - RR42_RS23755 0.43 -0.5 654,861 + RR42_RS23755 0.44 -0.3 654,862 - RR42_RS23755 0.44 -0.2 655,003 + RR42_RS23755 0.54 -0.5 655,004 - RR42_RS23755 0.54 -0.3 655,004 - RR42_RS23755 0.54 -0.9 655,004 - RR42_RS23755 0.54 -0.0 655,158 + RR42_RS23755 0.66 +1.0 655,159 - RR42_RS23755 0.66 -0.4 655,363 + RR42_RS23755 0.81 -1.0 655,363 + RR42_RS23755 0.81 +0.2 655,364 - RR42_RS23755 0.82 +0.2 655,392 + RR42_RS23755 0.84 -0.6 655,756 + -0.7 655,768 + RR42_RS23760 0.25 -0.7 655,784 + RR42_RS23760 0.46 +0.5 655,784 + RR42_RS23760 0.46 -1.1 655,837 - RR42_RS23765 0.11 -0.1 655,837 - RR42_RS23765 0.11 -0.4 655,849 - RR42_RS23765 0.26 +0.7 655,849 - RR42_RS23765 0.26 -0.1 655,925 + -1.2 655,925 + -0.6 655,926 - +0.2 655,931 - -0.3 656,010 + +1.5 656,011 - +1.5 656,017 - +0.4 656,017 - -0.5 656,058 + +1.9 656,058 + -0.9 656,058 + -0.9 656,058 + -1.2 656,058 + +0.6 656,071 + -1.4 656,083 + -0.1 656,083 + -0.2 656,083 + -0.6 656,083 + -0.6 656,084 - +0.3 656,084 - -0.5 656,149 + RR42_RS23770 0.15 +0.1 656,149 + RR42_RS23770 0.15 -0.0 656,177 + RR42_RS23770 0.18 -1.4 656,177 + RR42_RS23770 0.18 -1.4 656,178 - RR42_RS23770 0.19 +0.0 656,178 - RR42_RS23770 0.19 -1.2 656,178 - RR42_RS23770 0.19 -1.3 656,188 + RR42_RS23770 0.20 -1.4 656,189 - RR42_RS23770 0.20 -1.9 656,189 - RR42_RS23770 0.20 -0.6 656,189 - RR42_RS23770 0.20 -0.9 656,328 + RR42_RS23770 0.35 -1.1 656,330 + RR42_RS23770 0.35 -2.2 656,331 - RR42_RS23770 0.35 +0.8 656,341 - RR42_RS23770 0.36 +0.2 656,425 + RR42_RS23770 0.46 -0.7 656,425 + RR42_RS23770 0.46 -0.1 656,426 - RR42_RS23770 0.46 +0.4 656,426 - RR42_RS23770 0.46 -1.1 656,426 - RR42_RS23770 0.46 -1.4 656,444 + RR42_RS23770 0.48 -0.9 656,444 + RR42_RS23770 0.48 -0.8 656,445 - RR42_RS23770 0.48 -0.1 656,497 - RR42_RS23770 0.54 +0.2 656,530 + RR42_RS23770 0.57 +0.0 656,531 - RR42_RS23770 0.57 -0.5 656,531 - RR42_RS23770 0.57 -0.5 656,531 - RR42_RS23770 0.57 -0.4 656,573 + RR42_RS23770 0.62 -1.4 656,574 - RR42_RS23770 0.62 -1.5 656,583 - RR42_RS23770 0.63 -0.2 656,720 + RR42_RS23770 0.78 -1.9 656,726 - RR42_RS23770 0.79 -1.1 656,730 + RR42_RS23770 0.79 -1.4 656,730 + RR42_RS23770 0.79 -2.6 656,731 - RR42_RS23770 0.79 -1.1 656,731 - RR42_RS23770 0.79 +0.9 656,731 - RR42_RS23770 0.79 +0.0 656,777 - RR42_RS23770 0.84 -0.8 656,810 - RR42_RS23770 0.88 +0.4
Or see this region's nucleotide sequence