Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23700 and RR42_RS23705 are separated by 51 nucleotides RR42_RS23705 and RR42_RS23710 are separated by 40 nucleotides
RR42_RS23700: RR42_RS23700 - acyl-CoA dehydrogenase, at 643,022 to 644,173
_RS23700
RR42_RS23705: RR42_RS23705 - cyclohexanecarboxylate--CoA ligase, at 644,225 to 645,877
_RS23705
RR42_RS23710: RR42_RS23710 - enoyl-CoA hydratase, at 645,918 to 646,667
_RS23710
Position (kb)
644
645
646 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 643.274 kb on + strand, within RR42_RS23700 at 643.274 kb on + strand, within RR42_RS23700 at 643.274 kb on + strand, within RR42_RS23700 at 643.274 kb on + strand, within RR42_RS23700 at 643.274 kb on + strand, within RR42_RS23700 at 643.274 kb on + strand, within RR42_RS23700 at 643.275 kb on - strand, within RR42_RS23700 at 643.275 kb on - strand, within RR42_RS23700 at 643.460 kb on + strand, within RR42_RS23700 at 643.460 kb on + strand, within RR42_RS23700 at 643.460 kb on + strand, within RR42_RS23700 at 643.460 kb on + strand, within RR42_RS23700 at 643.461 kb on - strand, within RR42_RS23700 at 643.767 kb on + strand, within RR42_RS23700 at 643.808 kb on + strand, within RR42_RS23700 at 643.808 kb on + strand, within RR42_RS23700 at 643.808 kb on + strand, within RR42_RS23700 at 643.808 kb on + strand, within RR42_RS23700 at 643.809 kb on - strand, within RR42_RS23700 at 643.809 kb on - strand, within RR42_RS23700 at 643.809 kb on - strand, within RR42_RS23700 at 644.001 kb on - strand, within RR42_RS23700 at 644.001 kb on - strand, within RR42_RS23700 at 644.229 kb on - strand at 644.396 kb on + strand, within RR42_RS23705 at 644.514 kb on - strand, within RR42_RS23705 at 644.648 kb on + strand, within RR42_RS23705 at 644.648 kb on + strand, within RR42_RS23705 at 644.649 kb on - strand, within RR42_RS23705 at 644.816 kb on + strand, within RR42_RS23705 at 644.816 kb on + strand, within RR42_RS23705 at 644.817 kb on - strand, within RR42_RS23705 at 644.817 kb on - strand, within RR42_RS23705 at 644.817 kb on - strand, within RR42_RS23705 at 644.891 kb on + strand, within RR42_RS23705 at 644.892 kb on - strand, within RR42_RS23705 at 644.969 kb on + strand, within RR42_RS23705 at 644.969 kb on + strand, within RR42_RS23705 at 644.969 kb on + strand, within RR42_RS23705 at 644.970 kb on - strand, within RR42_RS23705 at 644.970 kb on - strand, within RR42_RS23705 at 644.970 kb on - strand, within RR42_RS23705 at 644.970 kb on - strand, within RR42_RS23705 at 645.043 kb on - strand, within RR42_RS23705 at 645.590 kb on + strand, within RR42_RS23705 at 645.591 kb on - strand, within RR42_RS23705 at 645.746 kb on + strand at 645.746 kb on + strand at 645.746 kb on + strand at 645.746 kb on + strand at 645.746 kb on + strand at 645.747 kb on - strand at 645.747 kb on - strand at 645.907 kb on + strand at 646.113 kb on + strand, within RR42_RS23710 at 646.113 kb on + strand, within RR42_RS23710 at 646.114 kb on - strand, within RR42_RS23710 at 646.114 kb on - strand, within RR42_RS23710 at 646.115 kb on + strand, within RR42_RS23710 at 646.115 kb on + strand, within RR42_RS23710 at 646.115 kb on + strand, within RR42_RS23710 at 646.115 kb on + strand, within RR42_RS23710 at 646.115 kb on + strand, within RR42_RS23710 at 646.115 kb on + strand, within RR42_RS23710 at 646.137 kb on + strand, within RR42_RS23710 at 646.137 kb on + strand, within RR42_RS23710 at 646.138 kb on - strand, within RR42_RS23710 at 646.138 kb on - strand, within RR42_RS23710 at 646.138 kb on - strand, within RR42_RS23710 at 646.420 kb on + strand, within RR42_RS23710 at 646.420 kb on + strand, within RR42_RS23710 at 646.666 kb on - strand at 646.789 kb on + strand at 646.789 kb on + strand at 646.790 kb on - strand at 646.792 kb on + strand at 646.793 kb on - strand at 646.803 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 643,274 + RR42_RS23700 0.22 -2.0 643,274 + RR42_RS23700 0.22 +0.5 643,274 + RR42_RS23700 0.22 -0.8 643,274 + RR42_RS23700 0.22 -0.0 643,274 + RR42_RS23700 0.22 -2.0 643,274 + RR42_RS23700 0.22 -0.4 643,275 - RR42_RS23700 0.22 -0.6 643,275 - RR42_RS23700 0.22 -1.3 643,460 + RR42_RS23700 0.38 -0.6 643,460 + RR42_RS23700 0.38 -1.8 643,460 + RR42_RS23700 0.38 -1.5 643,460 + RR42_RS23700 0.38 +0.3 643,461 - RR42_RS23700 0.38 +0.6 643,767 + RR42_RS23700 0.65 -1.8 643,808 + RR42_RS23700 0.68 +0.8 643,808 + RR42_RS23700 0.68 +0.4 643,808 + RR42_RS23700 0.68 +0.0 643,808 + RR42_RS23700 0.68 -0.5 643,809 - RR42_RS23700 0.68 -3.4 643,809 - RR42_RS23700 0.68 +0.1 643,809 - RR42_RS23700 0.68 -0.3 644,001 - RR42_RS23700 0.85 +1.0 644,001 - RR42_RS23700 0.85 -0.9 644,229 - -0.5 644,396 + RR42_RS23705 0.10 -0.4 644,514 - RR42_RS23705 0.17 -0.6 644,648 + RR42_RS23705 0.26 +1.9 644,648 + RR42_RS23705 0.26 -0.5 644,649 - RR42_RS23705 0.26 +0.5 644,816 + RR42_RS23705 0.36 +0.4 644,816 + RR42_RS23705 0.36 +0.2 644,817 - RR42_RS23705 0.36 -0.2 644,817 - RR42_RS23705 0.36 -1.7 644,817 - RR42_RS23705 0.36 -1.0 644,891 + RR42_RS23705 0.40 -1.6 644,892 - RR42_RS23705 0.40 -0.3 644,969 + RR42_RS23705 0.45 -0.3 644,969 + RR42_RS23705 0.45 -0.2 644,969 + RR42_RS23705 0.45 -0.6 644,970 - RR42_RS23705 0.45 +0.3 644,970 - RR42_RS23705 0.45 -2.8 644,970 - RR42_RS23705 0.45 -1.8 644,970 - RR42_RS23705 0.45 -0.3 645,043 - RR42_RS23705 0.49 -0.3 645,590 + RR42_RS23705 0.83 -1.2 645,591 - RR42_RS23705 0.83 -1.8 645,746 + -1.2 645,746 + -0.4 645,746 + -1.2 645,746 + +0.1 645,746 + -0.4 645,747 - +1.0 645,747 - -1.2 645,907 + -1.5 646,113 + RR42_RS23710 0.26 -1.6 646,113 + RR42_RS23710 0.26 +0.1 646,114 - RR42_RS23710 0.26 -0.4 646,114 - RR42_RS23710 0.26 -0.7 646,115 + RR42_RS23710 0.26 -0.1 646,115 + RR42_RS23710 0.26 -0.4 646,115 + RR42_RS23710 0.26 -1.2 646,115 + RR42_RS23710 0.26 -1.6 646,115 + RR42_RS23710 0.26 -0.0 646,115 + RR42_RS23710 0.26 -0.0 646,137 + RR42_RS23710 0.29 +0.2 646,137 + RR42_RS23710 0.29 +0.0 646,138 - RR42_RS23710 0.29 -0.6 646,138 - RR42_RS23710 0.29 -0.2 646,138 - RR42_RS23710 0.29 -2.1 646,420 + RR42_RS23710 0.67 -2.9 646,420 + RR42_RS23710 0.67 -0.3 646,666 - -0.6 646,789 + -0.5 646,789 + +0.4 646,790 - -0.8 646,792 + -0.4 646,793 - -0.6 646,803 + +0.4
Or see this region's nucleotide sequence