Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23080 and RR42_RS23085 are separated by 159 nucleotides RR42_RS23085 and RR42_RS23090 are separated by 33 nucleotides
RR42_RS23080: RR42_RS23080 - LysR family transcriptional regulator, at 512,656 to 513,591
_RS23080
RR42_RS23085: RR42_RS23085 - cytochrome C biogenesis protein CcdA, at 513,751 to 514,677
_RS23085
RR42_RS23090: RR42_RS23090 - 2,5-dioxovalerate dehydrogenase, at 514,711 to 516,312
_RS23090
Position (kb) 513
514
515 Strain fitness (log2 ratio) -2
-1
0
1
2 at 512.836 kb on + strand, within RR42_RS23080 at 512.837 kb on - strand, within RR42_RS23080 at 513.083 kb on + strand, within RR42_RS23080 at 513.083 kb on + strand, within RR42_RS23080 at 513.084 kb on - strand, within RR42_RS23080 at 513.090 kb on - strand, within RR42_RS23080 at 513.591 kb on + strand at 513.592 kb on - strand at 513.676 kb on - strand at 513.677 kb on + strand at 513.677 kb on + strand at 513.677 kb on + strand at 513.677 kb on + strand at 513.678 kb on - strand at 513.678 kb on - strand at 513.701 kb on + strand at 513.701 kb on + strand at 513.701 kb on + strand at 513.702 kb on - strand at 513.760 kb on - strand at 513.773 kb on - strand at 513.773 kb on - strand at 513.783 kb on - strand at 514.123 kb on + strand, within RR42_RS23085 at 514.123 kb on + strand, within RR42_RS23085 at 514.124 kb on - strand, within RR42_RS23085 at 514.124 kb on - strand, within RR42_RS23085 at 514.124 kb on - strand, within RR42_RS23085 at 514.158 kb on + strand, within RR42_RS23085 at 514.253 kb on - strand, within RR42_RS23085 at 514.284 kb on - strand, within RR42_RS23085 at 514.478 kb on - strand, within RR42_RS23085 at 514.478 kb on - strand, within RR42_RS23085 at 514.504 kb on + strand, within RR42_RS23085 at 514.504 kb on + strand, within RR42_RS23085 at 514.504 kb on + strand, within RR42_RS23085 at 514.505 kb on - strand, within RR42_RS23085 at 514.505 kb on - strand, within RR42_RS23085 at 515.018 kb on + strand, within RR42_RS23090 at 515.284 kb on - strand, within RR42_RS23090 at 515.497 kb on + strand, within RR42_RS23090
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 512,836 + RR42_RS23080 0.19 -0.6 512,837 - RR42_RS23080 0.19 +0.3 513,083 + RR42_RS23080 0.46 +0.0 513,083 + RR42_RS23080 0.46 +0.5 513,084 - RR42_RS23080 0.46 -0.4 513,090 - RR42_RS23080 0.46 -0.4 513,591 + -1.1 513,592 - +1.8 513,676 - +0.0 513,677 + -1.6 513,677 + +0.2 513,677 + -0.2 513,677 + -0.5 513,678 - +0.5 513,678 - +0.1 513,701 + -0.5 513,701 + +0.6 513,701 + +2.0 513,702 - +0.5 513,760 - +0.1 513,773 - -0.4 513,773 - -1.3 513,783 - +0.8 514,123 + RR42_RS23085 0.40 -2.2 514,123 + RR42_RS23085 0.40 -0.6 514,124 - RR42_RS23085 0.40 -0.3 514,124 - RR42_RS23085 0.40 -1.2 514,124 - RR42_RS23085 0.40 +0.0 514,158 + RR42_RS23085 0.44 +0.6 514,253 - RR42_RS23085 0.54 +0.0 514,284 - RR42_RS23085 0.57 -0.8 514,478 - RR42_RS23085 0.78 -0.2 514,478 - RR42_RS23085 0.78 -0.6 514,504 + RR42_RS23085 0.81 -0.6 514,504 + RR42_RS23085 0.81 +0.2 514,504 + RR42_RS23085 0.81 +0.1 514,505 - RR42_RS23085 0.81 +0.8 514,505 - RR42_RS23085 0.81 +0.2 515,018 + RR42_RS23090 0.19 +0.2 515,284 - RR42_RS23090 0.36 +0.1 515,497 + RR42_RS23090 0.49 -2.4
Or see this region's nucleotide sequence