Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22935 and RR42_RS22940 are separated by 60 nucleotides RR42_RS22940 and RR42_RS22945 are separated by 137 nucleotides
RR42_RS22935: RR42_RS22935 - metal transporter, at 479,396 to 480,613
_RS22935
RR42_RS22940: RR42_RS22940 - TetR family transcriptional regulator, at 480,674 to 481,348
_RS22940
RR42_RS22945: RR42_RS22945 - oxidoreductase, at 481,486 to 482,514
_RS22945
Position (kb) 480
481
482 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 479.866 kb on - strand, within RR42_RS22935 at 480.080 kb on + strand, within RR42_RS22935 at 480.080 kb on + strand, within RR42_RS22935 at 480.081 kb on - strand, within RR42_RS22935 at 480.458 kb on + strand, within RR42_RS22935 at 480.661 kb on + strand at 480.819 kb on - strand, within RR42_RS22940 at 481.130 kb on - strand, within RR42_RS22940 at 481.182 kb on + strand, within RR42_RS22940 at 481.183 kb on - strand, within RR42_RS22940 at 481.735 kb on + strand, within RR42_RS22945 at 481.735 kb on + strand, within RR42_RS22945 at 481.735 kb on + strand, within RR42_RS22945 at 481.735 kb on + strand, within RR42_RS22945 at 481.735 kb on + strand, within RR42_RS22945 at 481.735 kb on + strand, within RR42_RS22945 at 481.736 kb on - strand, within RR42_RS22945 at 481.810 kb on + strand, within RR42_RS22945 at 481.811 kb on - strand, within RR42_RS22945 at 481.924 kb on + strand, within RR42_RS22945 at 481.924 kb on + strand, within RR42_RS22945 at 481.924 kb on + strand, within RR42_RS22945 at 481.924 kb on + strand, within RR42_RS22945 at 481.925 kb on - strand, within RR42_RS22945 at 481.927 kb on + strand, within RR42_RS22945 at 481.928 kb on - strand, within RR42_RS22945 at 482.205 kb on + strand, within RR42_RS22945 at 482.205 kb on + strand, within RR42_RS22945 at 482.205 kb on + strand, within RR42_RS22945 at 482.206 kb on - strand, within RR42_RS22945 at 482.206 kb on - strand, within RR42_RS22945 at 482.317 kb on + strand, within RR42_RS22945
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 479,866 - RR42_RS22935 0.39 -0.5 480,080 + RR42_RS22935 0.56 -0.8 480,080 + RR42_RS22935 0.56 -0.8 480,081 - RR42_RS22935 0.56 -1.5 480,458 + RR42_RS22935 0.87 -0.7 480,661 + -2.3 480,819 - RR42_RS22940 0.21 +0.2 481,130 - RR42_RS22940 0.68 -0.2 481,182 + RR42_RS22940 0.75 -0.8 481,183 - RR42_RS22940 0.75 -3.1 481,735 + RR42_RS22945 0.24 +0.7 481,735 + RR42_RS22945 0.24 +2.8 481,735 + RR42_RS22945 0.24 -0.6 481,735 + RR42_RS22945 0.24 -0.1 481,735 + RR42_RS22945 0.24 -0.4 481,735 + RR42_RS22945 0.24 +0.5 481,736 - RR42_RS22945 0.24 -1.0 481,810 + RR42_RS22945 0.31 +0.2 481,811 - RR42_RS22945 0.32 -3.3 481,924 + RR42_RS22945 0.43 -0.6 481,924 + RR42_RS22945 0.43 -0.3 481,924 + RR42_RS22945 0.43 -1.2 481,924 + RR42_RS22945 0.43 -1.9 481,925 - RR42_RS22945 0.43 -2.0 481,927 + RR42_RS22945 0.43 +1.2 481,928 - RR42_RS22945 0.43 -2.2 482,205 + RR42_RS22945 0.70 +0.3 482,205 + RR42_RS22945 0.70 -0.5 482,205 + RR42_RS22945 0.70 +1.1 482,206 - RR42_RS22945 0.70 +0.9 482,206 - RR42_RS22945 0.70 +1.5 482,317 + RR42_RS22945 0.81 -1.0
Or see this region's nucleotide sequence