Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22690 and RR42_RS22695 are separated by 316 nucleotides RR42_RS22695 and RR42_RS22700 are separated by 193 nucleotides
RR42_RS22690: RR42_RS22690 - CoA-transferase, at 425,401 to 426,582
_RS22690
RR42_RS22695: RR42_RS22695 - chemotaxis protein, at 426,899 to 427,324
_RS22695
RR42_RS22700: RR42_RS22700 - phasin, at 427,518 to 428,075
_RS22700
Position (kb)
426
427
428 Strain fitness (log2 ratio)
-2
-1
0
1 at 426.008 kb on + strand, within RR42_RS22690 at 426.008 kb on + strand, within RR42_RS22690 at 426.008 kb on + strand, within RR42_RS22690 at 426.008 kb on + strand, within RR42_RS22690 at 426.008 kb on + strand, within RR42_RS22690 at 426.008 kb on + strand, within RR42_RS22690 at 426.008 kb on + strand, within RR42_RS22690 at 426.009 kb on - strand, within RR42_RS22690 at 426.009 kb on - strand, within RR42_RS22690 at 426.009 kb on - strand, within RR42_RS22690 at 426.009 kb on - strand, within RR42_RS22690 at 426.009 kb on - strand, within RR42_RS22690 at 426.009 kb on - strand, within RR42_RS22690 at 426.009 kb on - strand, within RR42_RS22690 at 426.009 kb on - strand, within RR42_RS22690 at 426.089 kb on + strand, within RR42_RS22690 at 426.090 kb on - strand, within RR42_RS22690 at 426.122 kb on + strand, within RR42_RS22690 at 426.122 kb on + strand, within RR42_RS22690 at 426.123 kb on - strand, within RR42_RS22690 at 426.123 kb on - strand, within RR42_RS22690 at 426.123 kb on - strand, within RR42_RS22690 at 426.123 kb on - strand, within RR42_RS22690 at 426.182 kb on + strand, within RR42_RS22690 at 426.218 kb on + strand, within RR42_RS22690 at 426.254 kb on + strand, within RR42_RS22690 at 426.255 kb on - strand, within RR42_RS22690 at 426.255 kb on - strand, within RR42_RS22690 at 426.324 kb on - strand, within RR42_RS22690 at 426.368 kb on + strand, within RR42_RS22690 at 426.425 kb on + strand, within RR42_RS22690 at 426.425 kb on + strand, within RR42_RS22690 at 426.426 kb on - strand, within RR42_RS22690 at 426.472 kb on + strand at 426.472 kb on + strand at 426.472 kb on + strand at 426.472 kb on + strand at 426.473 kb on - strand at 426.532 kb on + strand at 426.533 kb on - strand at 426.653 kb on + strand at 426.653 kb on + strand at 426.654 kb on - strand at 426.654 kb on - strand at 426.792 kb on - strand at 426.950 kb on + strand, within RR42_RS22695 at 426.951 kb on - strand, within RR42_RS22695 at 426.970 kb on + strand, within RR42_RS22695 at 426.971 kb on - strand, within RR42_RS22695 at 427.204 kb on + strand, within RR42_RS22695 at 427.204 kb on + strand, within RR42_RS22695 at 427.322 kb on + strand at 427.383 kb on + strand at 427.639 kb on + strand, within RR42_RS22700 at 427.640 kb on - strand, within RR42_RS22700 at 427.759 kb on + strand, within RR42_RS22700 at 427.813 kb on + strand, within RR42_RS22700 at 427.813 kb on + strand, within RR42_RS22700 at 427.813 kb on + strand, within RR42_RS22700 at 427.814 kb on - strand, within RR42_RS22700 at 427.828 kb on + strand, within RR42_RS22700 at 427.865 kb on + strand, within RR42_RS22700 at 427.866 kb on - strand, within RR42_RS22700 at 427.866 kb on - strand, within RR42_RS22700 at 428.000 kb on + strand, within RR42_RS22700 at 428.001 kb on - strand, within RR42_RS22700 at 428.001 kb on - strand, within RR42_RS22700 at 428.091 kb on + strand at 428.213 kb on + strand at 428.223 kb on + strand at 428.263 kb on + strand at 428.264 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 426,008 + RR42_RS22690 0.51 +0.4 426,008 + RR42_RS22690 0.51 -0.1 426,008 + RR42_RS22690 0.51 -0.6 426,008 + RR42_RS22690 0.51 -1.0 426,008 + RR42_RS22690 0.51 -0.8 426,008 + RR42_RS22690 0.51 +0.8 426,008 + RR42_RS22690 0.51 -0.1 426,009 - RR42_RS22690 0.51 -1.8 426,009 - RR42_RS22690 0.51 +0.1 426,009 - RR42_RS22690 0.51 +0.6 426,009 - RR42_RS22690 0.51 -0.1 426,009 - RR42_RS22690 0.51 -0.6 426,009 - RR42_RS22690 0.51 -0.7 426,009 - RR42_RS22690 0.51 +0.1 426,009 - RR42_RS22690 0.51 -0.3 426,089 + RR42_RS22690 0.58 -0.4 426,090 - RR42_RS22690 0.58 +0.4 426,122 + RR42_RS22690 0.61 -0.6 426,122 + RR42_RS22690 0.61 -0.4 426,123 - RR42_RS22690 0.61 -0.5 426,123 - RR42_RS22690 0.61 -0.0 426,123 - RR42_RS22690 0.61 -0.6 426,123 - RR42_RS22690 0.61 -0.2 426,182 + RR42_RS22690 0.66 +0.2 426,218 + RR42_RS22690 0.69 +0.1 426,254 + RR42_RS22690 0.72 -2.6 426,255 - RR42_RS22690 0.72 +1.5 426,255 - RR42_RS22690 0.72 +0.6 426,324 - RR42_RS22690 0.78 +0.4 426,368 + RR42_RS22690 0.82 -0.0 426,425 + RR42_RS22690 0.87 +0.1 426,425 + RR42_RS22690 0.87 -0.2 426,426 - RR42_RS22690 0.87 -0.5 426,472 + +0.7 426,472 + +0.5 426,472 + -1.5 426,472 + +0.1 426,473 - +0.4 426,532 + -1.4 426,533 - -1.2 426,653 + +0.3 426,653 + -0.0 426,654 - -0.1 426,654 - +0.2 426,792 - -0.4 426,950 + RR42_RS22695 0.12 +0.1 426,951 - RR42_RS22695 0.12 +0.9 426,970 + RR42_RS22695 0.17 -0.8 426,971 - RR42_RS22695 0.17 -0.8 427,204 + RR42_RS22695 0.72 -0.5 427,204 + RR42_RS22695 0.72 -0.9 427,322 + -0.3 427,383 + +0.0 427,639 + RR42_RS22700 0.22 -1.0 427,640 - RR42_RS22700 0.22 -0.1 427,759 + RR42_RS22700 0.43 +0.5 427,813 + RR42_RS22700 0.53 +0.9 427,813 + RR42_RS22700 0.53 -1.4 427,813 + RR42_RS22700 0.53 +0.0 427,814 - RR42_RS22700 0.53 +0.5 427,828 + RR42_RS22700 0.56 -0.3 427,865 + RR42_RS22700 0.62 -0.8 427,866 - RR42_RS22700 0.62 +0.4 427,866 - RR42_RS22700 0.62 -0.6 428,000 + RR42_RS22700 0.86 -1.1 428,001 - RR42_RS22700 0.87 +0.5 428,001 - RR42_RS22700 0.87 -0.2 428,091 + -0.2 428,213 + -1.4 428,223 + -0.4 428,263 + +0.2 428,264 - -0.6
Or see this region's nucleotide sequence