Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21060 and RR42_RS21065 are separated by 53 nucleotides RR42_RS21065 and RR42_RS21070 are separated by 526 nucleotides
RR42_RS21060: RR42_RS21060 - histidine kinase, at 49,245 to 51,272
_RS21060
RR42_RS21065: RR42_RS21065 - diguanylate phosphodiesterase, at 51,326 to 52,942
_RS21065
RR42_RS21070: RR42_RS21070 - hypothetical protein, at 53,469 to 53,651
_RS21070
Position (kb)
51
52
53 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 50.514 kb on + strand, within RR42_RS21060 at 50.515 kb on - strand, within RR42_RS21060 at 50.515 kb on - strand, within RR42_RS21060 at 50.515 kb on - strand, within RR42_RS21060 at 50.836 kb on + strand, within RR42_RS21060 at 50.836 kb on + strand, within RR42_RS21060 at 50.969 kb on - strand, within RR42_RS21060 at 50.986 kb on - strand, within RR42_RS21060 at 50.986 kb on - strand, within RR42_RS21060 at 50.986 kb on - strand, within RR42_RS21060 at 50.986 kb on - strand, within RR42_RS21060 at 51.034 kb on + strand, within RR42_RS21060 at 51.143 kb on + strand at 51.143 kb on + strand at 51.144 kb on - strand at 51.144 kb on - strand at 51.144 kb on - strand at 51.144 kb on - strand at 51.145 kb on + strand at 51.146 kb on - strand at 51.146 kb on - strand at 51.357 kb on + strand at 51.539 kb on + strand, within RR42_RS21065 at 51.539 kb on + strand, within RR42_RS21065 at 51.540 kb on - strand, within RR42_RS21065 at 51.540 kb on - strand, within RR42_RS21065 at 51.548 kb on + strand, within RR42_RS21065 at 51.548 kb on + strand, within RR42_RS21065 at 51.548 kb on + strand, within RR42_RS21065 at 51.551 kb on - strand, within RR42_RS21065 at 51.588 kb on - strand, within RR42_RS21065 at 51.588 kb on - strand, within RR42_RS21065 at 51.735 kb on + strand, within RR42_RS21065 at 51.736 kb on - strand, within RR42_RS21065 at 51.758 kb on + strand, within RR42_RS21065 at 51.759 kb on - strand, within RR42_RS21065 at 52.032 kb on + strand, within RR42_RS21065 at 52.033 kb on - strand, within RR42_RS21065 at 52.052 kb on + strand, within RR42_RS21065 at 52.052 kb on + strand, within RR42_RS21065 at 52.052 kb on + strand, within RR42_RS21065 at 52.052 kb on + strand, within RR42_RS21065 at 52.053 kb on - strand, within RR42_RS21065 at 52.053 kb on - strand, within RR42_RS21065 at 52.184 kb on + strand, within RR42_RS21065 at 52.188 kb on - strand, within RR42_RS21065 at 52.188 kb on - strand, within RR42_RS21065 at 52.823 kb on + strand at 52.823 kb on + strand at 52.824 kb on - strand at 53.033 kb on + strand at 53.034 kb on - strand at 53.034 kb on - strand at 53.100 kb on - strand at 53.172 kb on + strand at 53.207 kb on + strand at 53.207 kb on + strand at 53.208 kb on - strand at 53.208 kb on - strand at 53.249 kb on + strand at 53.332 kb on - strand at 53.470 kb on + strand at 53.471 kb on - strand at 53.489 kb on - strand, within RR42_RS21070 at 53.489 kb on - strand, within RR42_RS21070 at 53.522 kb on + strand, within RR42_RS21070 at 53.523 kb on - strand, within RR42_RS21070 at 53.523 kb on - strand, within RR42_RS21070 at 53.580 kb on + strand, within RR42_RS21070 at 53.581 kb on - strand, within RR42_RS21070 at 53.581 kb on - strand, within RR42_RS21070 at 53.645 kb on + strand at 53.645 kb on + strand at 53.646 kb on - strand at 53.646 kb on - strand at 53.646 kb on - strand at 53.776 kb on - strand at 53.794 kb on + strand at 53.794 kb on + strand at 53.795 kb on - strand at 53.795 kb on - strand at 53.795 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 50,514 + RR42_RS21060 0.63 +0.4 50,515 - RR42_RS21060 0.63 -0.6 50,515 - RR42_RS21060 0.63 -0.5 50,515 - RR42_RS21060 0.63 -0.8 50,836 + RR42_RS21060 0.78 +0.0 50,836 + RR42_RS21060 0.78 -0.3 50,969 - RR42_RS21060 0.85 +0.1 50,986 - RR42_RS21060 0.86 +0.1 50,986 - RR42_RS21060 0.86 +0.3 50,986 - RR42_RS21060 0.86 -0.7 50,986 - RR42_RS21060 0.86 -0.3 51,034 + RR42_RS21060 0.88 -0.8 51,143 + -0.8 51,143 + -0.6 51,144 - +0.2 51,144 - -0.4 51,144 - -0.5 51,144 - -0.2 51,145 + -0.2 51,146 - -0.1 51,146 - -0.0 51,357 + +0.5 51,539 + RR42_RS21065 0.13 -0.2 51,539 + RR42_RS21065 0.13 -0.1 51,540 - RR42_RS21065 0.13 +0.0 51,540 - RR42_RS21065 0.13 +0.0 51,548 + RR42_RS21065 0.14 -0.6 51,548 + RR42_RS21065 0.14 +2.1 51,548 + RR42_RS21065 0.14 -0.0 51,551 - RR42_RS21065 0.14 -1.4 51,588 - RR42_RS21065 0.16 -0.8 51,588 - RR42_RS21065 0.16 -0.8 51,735 + RR42_RS21065 0.25 -1.0 51,736 - RR42_RS21065 0.25 -0.1 51,758 + RR42_RS21065 0.27 -1.0 51,759 - RR42_RS21065 0.27 -1.5 52,032 + RR42_RS21065 0.44 -0.5 52,033 - RR42_RS21065 0.44 -0.8 52,052 + RR42_RS21065 0.45 -1.6 52,052 + RR42_RS21065 0.45 -0.9 52,052 + RR42_RS21065 0.45 -0.5 52,052 + RR42_RS21065 0.45 -0.4 52,053 - RR42_RS21065 0.45 -0.9 52,053 - RR42_RS21065 0.45 +0.4 52,184 + RR42_RS21065 0.53 +0.4 52,188 - RR42_RS21065 0.53 +3.2 52,188 - RR42_RS21065 0.53 -0.4 52,823 + +0.5 52,823 + -0.2 52,824 - +0.4 53,033 + -0.1 53,034 - -1.3 53,034 - +0.4 53,100 - -2.1 53,172 + -0.1 53,207 + -0.6 53,207 + -0.6 53,208 - +0.3 53,208 - +0.3 53,249 + +1.2 53,332 - +0.1 53,470 + -0.3 53,471 - -0.2 53,489 - RR42_RS21070 0.11 -0.5 53,489 - RR42_RS21070 0.11 +0.0 53,522 + RR42_RS21070 0.29 -0.3 53,523 - RR42_RS21070 0.30 -2.0 53,523 - RR42_RS21070 0.30 +0.1 53,580 + RR42_RS21070 0.61 -0.0 53,581 - RR42_RS21070 0.61 -0.6 53,581 - RR42_RS21070 0.61 -0.2 53,645 + +0.8 53,645 + +0.9 53,646 - +0.6 53,646 - -1.8 53,646 - -2.0 53,776 - -1.4 53,794 + +0.8 53,794 + -0.4 53,795 - -0.8 53,795 - -0.5 53,795 - -0.2
Or see this region's nucleotide sequence