Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS20725

Experiment: R2A with Dimethyl Sulfoxide 5 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS20715 and RR42_RS20720 are separated by 55 nucleotidesRR42_RS20720 and RR42_RS20725 overlap by 4 nucleotidesRR42_RS20725 and RR42_RS20730 are separated by 61 nucleotidesRR42_RS20730 and RR42_RS20735 overlap by 4 nucleotides RR42_RS20715: RR42_RS20715 - 4-hydroxyacetophenone monooxygenase, at 4,498,637 to 4,500,211 _RS20715 RR42_RS20720: RR42_RS20720 - short-chain dehydrogenase, at 4,500,267 to 4,501,181 _RS20720 RR42_RS20725: RR42_RS20725 - alpha/beta hydrolase, at 4,501,178 to 4,502,140 _RS20725 RR42_RS20730: RR42_RS20730 - metal-dependent hydrolase, at 4,502,202 to 4,503,074 _RS20730 RR42_RS20735: RR42_RS20735 - rubredoxin, at 4,503,071 to 4,503,235 _RS20735 Position (kb) 4501 4502 4503Strain fitness (log2 ratio) -2 -1 0 1 2at 4500.463 kb on + strand, within RR42_RS20720at 4500.464 kb on - strand, within RR42_RS20720at 4500.483 kb on + strand, within RR42_RS20720at 4500.483 kb on + strand, within RR42_RS20720at 4500.483 kb on + strand, within RR42_RS20720at 4500.484 kb on - strand, within RR42_RS20720at 4500.484 kb on - strand, within RR42_RS20720at 4500.484 kb on - strand, within RR42_RS20720at 4500.582 kb on + strand, within RR42_RS20720at 4500.582 kb on + strand, within RR42_RS20720at 4500.582 kb on + strand, within RR42_RS20720at 4500.583 kb on - strand, within RR42_RS20720at 4500.583 kb on - strand, within RR42_RS20720at 4500.583 kb on - strand, within RR42_RS20720at 4500.583 kb on - strand, within RR42_RS20720at 4500.690 kb on - strand, within RR42_RS20720at 4500.690 kb on - strand, within RR42_RS20720at 4500.750 kb on + strand, within RR42_RS20720at 4500.936 kb on + strand, within RR42_RS20720at 4500.936 kb on + strand, within RR42_RS20720at 4500.937 kb on - strand, within RR42_RS20720at 4501.302 kb on - strand, within RR42_RS20725at 4501.302 kb on - strand, within RR42_RS20725at 4501.352 kb on + strand, within RR42_RS20725at 4501.353 kb on - strand, within RR42_RS20725at 4501.353 kb on - strand, within RR42_RS20725at 4501.353 kb on - strand, within RR42_RS20725at 4501.546 kb on + strand, within RR42_RS20725at 4501.547 kb on - strand, within RR42_RS20725at 4501.550 kb on + strand, within RR42_RS20725at 4501.550 kb on + strand, within RR42_RS20725at 4501.550 kb on + strand, within RR42_RS20725at 4501.587 kb on - strand, within RR42_RS20725at 4501.721 kb on + strand, within RR42_RS20725at 4501.721 kb on + strand, within RR42_RS20725at 4501.721 kb on + strand, within RR42_RS20725at 4501.724 kb on - strand, within RR42_RS20725at 4501.811 kb on + strand, within RR42_RS20725at 4502.058 kb on - strandat 4502.194 kb on + strandat 4502.194 kb on + strandat 4502.194 kb on + strandat 4502.195 kb on - strandat 4502.405 kb on + strand, within RR42_RS20730at 4502.438 kb on + strand, within RR42_RS20730at 4502.438 kb on + strand, within RR42_RS20730at 4502.439 kb on - strand, within RR42_RS20730at 4502.446 kb on - strand, within RR42_RS20730at 4502.446 kb on - strand, within RR42_RS20730at 4502.446 kb on - strand, within RR42_RS20730at 4502.531 kb on - strand, within RR42_RS20730at 4502.700 kb on + strand, within RR42_RS20730at 4502.701 kb on - strand, within RR42_RS20730at 4502.736 kb on + strand, within RR42_RS20730at 4502.737 kb on - strand, within RR42_RS20730at 4502.743 kb on - strand, within RR42_RS20730at 4502.931 kb on + strand, within RR42_RS20730at 4503.107 kb on + strand, within RR42_RS20735

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol%
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4,500,463 + RR42_RS20720 0.21 -1.9
4,500,464 - RR42_RS20720 0.22 -0.6
4,500,483 + RR42_RS20720 0.24 -0.3
4,500,483 + RR42_RS20720 0.24 -1.2
4,500,483 + RR42_RS20720 0.24 +0.2
4,500,484 - RR42_RS20720 0.24 +0.6
4,500,484 - RR42_RS20720 0.24 -1.5
4,500,484 - RR42_RS20720 0.24 +0.8
4,500,582 + RR42_RS20720 0.34 -0.9
4,500,582 + RR42_RS20720 0.34 -2.0
4,500,582 + RR42_RS20720 0.34 +0.1
4,500,583 - RR42_RS20720 0.35 -0.6
4,500,583 - RR42_RS20720 0.35 +0.1
4,500,583 - RR42_RS20720 0.35 -0.4
4,500,583 - RR42_RS20720 0.35 +1.8
4,500,690 - RR42_RS20720 0.46 +0.7
4,500,690 - RR42_RS20720 0.46 +1.1
4,500,750 + RR42_RS20720 0.53 -0.3
4,500,936 + RR42_RS20720 0.73 +0.7
4,500,936 + RR42_RS20720 0.73 +0.0
4,500,937 - RR42_RS20720 0.73 -0.3
4,501,302 - RR42_RS20725 0.13 +0.6
4,501,302 - RR42_RS20725 0.13 +0.1
4,501,352 + RR42_RS20725 0.18 -0.8
4,501,353 - RR42_RS20725 0.18 -1.0
4,501,353 - RR42_RS20725 0.18 -0.9
4,501,353 - RR42_RS20725 0.18 +1.5
4,501,546 + RR42_RS20725 0.38 -0.6
4,501,547 - RR42_RS20725 0.38 +0.3
4,501,550 + RR42_RS20725 0.39 +1.3
4,501,550 + RR42_RS20725 0.39 +1.1
4,501,550 + RR42_RS20725 0.39 +0.2
4,501,587 - RR42_RS20725 0.42 +0.6
4,501,721 + RR42_RS20725 0.56 +0.4
4,501,721 + RR42_RS20725 0.56 +0.2
4,501,721 + RR42_RS20725 0.56 -1.0
4,501,724 - RR42_RS20725 0.57 +1.0
4,501,811 + RR42_RS20725 0.66 +1.1
4,502,058 - -2.5
4,502,194 + -0.1
4,502,194 + +0.0
4,502,194 + -0.7
4,502,195 - -0.8
4,502,405 + RR42_RS20730 0.23 +0.8
4,502,438 + RR42_RS20730 0.27 +0.5
4,502,438 + RR42_RS20730 0.27 -0.2
4,502,439 - RR42_RS20730 0.27 +0.3
4,502,446 - RR42_RS20730 0.28 +0.5
4,502,446 - RR42_RS20730 0.28 -0.1
4,502,446 - RR42_RS20730 0.28 +0.5
4,502,531 - RR42_RS20730 0.38 +0.3
4,502,700 + RR42_RS20730 0.57 +0.2
4,502,701 - RR42_RS20730 0.57 +0.5
4,502,736 + RR42_RS20730 0.61 -0.4
4,502,737 - RR42_RS20730 0.61 +0.1
4,502,743 - RR42_RS20730 0.62 -1.0
4,502,931 + RR42_RS20730 0.84 -0.1
4,503,107 + RR42_RS20735 0.22 +1.7

Or see this region's nucleotide sequence