Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS20020 and RR42_RS20025 are separated by 64 nucleotides RR42_RS20025 and RR42_RS20030 are separated by 207 nucleotides
RR42_RS20020: RR42_RS20020 - cytochrome C, at 4,324,146 to 4,325,027
_RS20020
RR42_RS20025: RR42_RS20025 - coproporphyrinogen III oxidase, at 4,325,092 to 4,326,693
_RS20025
RR42_RS20030: RR42_RS20030 - ABC transporter permease, at 4,326,901 to 4,328,043
_RS20030
Position (kb)
4325
4326
4327 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4324.093 kb on + strand at 4324.093 kb on + strand at 4324.093 kb on + strand at 4324.093 kb on + strand at 4324.093 kb on + strand at 4324.093 kb on + strand at 4324.093 kb on + strand at 4324.093 kb on + strand at 4324.094 kb on - strand at 4324.145 kb on + strand at 4324.145 kb on + strand at 4324.146 kb on - strand at 4324.311 kb on - strand, within RR42_RS20020 at 4324.407 kb on + strand, within RR42_RS20020 at 4324.408 kb on - strand, within RR42_RS20020 at 4324.408 kb on - strand, within RR42_RS20020 at 4324.408 kb on - strand, within RR42_RS20020 at 4324.408 kb on - strand, within RR42_RS20020 at 4324.408 kb on - strand, within RR42_RS20020 at 4324.461 kb on + strand, within RR42_RS20020 at 4324.462 kb on - strand, within RR42_RS20020 at 4324.579 kb on - strand, within RR42_RS20020 at 4324.611 kb on + strand, within RR42_RS20020 at 4324.611 kb on + strand, within RR42_RS20020 at 4324.611 kb on + strand, within RR42_RS20020 at 4324.809 kb on + strand, within RR42_RS20020 at 4324.809 kb on + strand, within RR42_RS20020 at 4325.004 kb on + strand at 4325.004 kb on + strand at 4325.004 kb on + strand at 4325.004 kb on + strand at 4325.004 kb on + strand at 4325.004 kb on + strand at 4325.005 kb on - strand at 4325.026 kb on - strand at 4325.109 kb on - strand at 4325.133 kb on + strand at 4325.134 kb on - strand at 4325.480 kb on + strand, within RR42_RS20025 at 4325.480 kb on + strand, within RR42_RS20025 at 4325.481 kb on - strand, within RR42_RS20025 at 4325.483 kb on + strand, within RR42_RS20025 at 4325.483 kb on + strand, within RR42_RS20025 at 4325.484 kb on - strand, within RR42_RS20025 at 4325.484 kb on - strand, within RR42_RS20025 at 4325.513 kb on + strand, within RR42_RS20025 at 4325.583 kb on - strand, within RR42_RS20025 at 4325.583 kb on - strand, within RR42_RS20025 at 4325.669 kb on + strand, within RR42_RS20025 at 4325.669 kb on + strand, within RR42_RS20025 at 4325.669 kb on + strand, within RR42_RS20025 at 4325.669 kb on + strand, within RR42_RS20025 at 4325.669 kb on + strand, within RR42_RS20025 at 4325.670 kb on - strand, within RR42_RS20025 at 4325.670 kb on - strand, within RR42_RS20025 at 4325.670 kb on - strand, within RR42_RS20025 at 4325.670 kb on - strand, within RR42_RS20025 at 4325.670 kb on - strand, within RR42_RS20025 at 4325.675 kb on + strand, within RR42_RS20025 at 4325.795 kb on + strand, within RR42_RS20025 at 4325.801 kb on + strand, within RR42_RS20025 at 4325.802 kb on - strand, within RR42_RS20025 at 4325.885 kb on + strand, within RR42_RS20025 at 4325.886 kb on - strand, within RR42_RS20025 at 4325.886 kb on - strand, within RR42_RS20025 at 4326.270 kb on - strand, within RR42_RS20025 at 4326.324 kb on - strand, within RR42_RS20025 at 4326.356 kb on + strand, within RR42_RS20025 at 4326.356 kb on + strand, within RR42_RS20025 at 4326.356 kb on + strand, within RR42_RS20025 at 4326.357 kb on - strand, within RR42_RS20025 at 4326.357 kb on - strand, within RR42_RS20025 at 4326.357 kb on - strand, within RR42_RS20025 at 4326.357 kb on - strand, within RR42_RS20025 at 4326.491 kb on + strand, within RR42_RS20025 at 4326.508 kb on - strand, within RR42_RS20025 at 4326.729 kb on + strand at 4327.082 kb on - strand, within RR42_RS20030 at 4327.082 kb on - strand, within RR42_RS20030 at 4327.409 kb on - strand, within RR42_RS20030 at 4327.409 kb on - strand, within RR42_RS20030 at 4327.409 kb on - strand, within RR42_RS20030 at 4327.660 kb on + strand, within RR42_RS20030
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 4,324,093 + +0.3 4,324,093 + -1.3 4,324,093 + -0.7 4,324,093 + -0.4 4,324,093 + +0.3 4,324,093 + -1.1 4,324,093 + -0.2 4,324,093 + -2.0 4,324,094 - -0.9 4,324,145 + +2.6 4,324,145 + -1.1 4,324,146 - -0.5 4,324,311 - RR42_RS20020 0.19 -1.2 4,324,407 + RR42_RS20020 0.30 -1.1 4,324,408 - RR42_RS20020 0.30 -1.8 4,324,408 - RR42_RS20020 0.30 +0.1 4,324,408 - RR42_RS20020 0.30 -0.4 4,324,408 - RR42_RS20020 0.30 +1.3 4,324,408 - RR42_RS20020 0.30 -1.3 4,324,461 + RR42_RS20020 0.36 +0.2 4,324,462 - RR42_RS20020 0.36 -1.9 4,324,579 - RR42_RS20020 0.49 -1.8 4,324,611 + RR42_RS20020 0.53 -0.1 4,324,611 + RR42_RS20020 0.53 -0.9 4,324,611 + RR42_RS20020 0.53 -2.0 4,324,809 + RR42_RS20020 0.75 +1.4 4,324,809 + RR42_RS20020 0.75 -0.7 4,325,004 + -0.3 4,325,004 + +0.2 4,325,004 + -1.0 4,325,004 + -1.1 4,325,004 + -0.5 4,325,004 + -0.1 4,325,005 - +0.5 4,325,026 - -0.9 4,325,109 - -0.8 4,325,133 + +0.3 4,325,134 - -0.1 4,325,480 + RR42_RS20025 0.24 +0.2 4,325,480 + RR42_RS20025 0.24 -0.8 4,325,481 - RR42_RS20025 0.24 -1.1 4,325,483 + RR42_RS20025 0.24 +0.7 4,325,483 + RR42_RS20025 0.24 -1.3 4,325,484 - RR42_RS20025 0.24 -0.3 4,325,484 - RR42_RS20025 0.24 +0.8 4,325,513 + RR42_RS20025 0.26 -0.6 4,325,583 - RR42_RS20025 0.31 -0.7 4,325,583 - RR42_RS20025 0.31 -0.3 4,325,669 + RR42_RS20025 0.36 -0.7 4,325,669 + RR42_RS20025 0.36 -0.9 4,325,669 + RR42_RS20025 0.36 +0.4 4,325,669 + RR42_RS20025 0.36 -0.1 4,325,669 + RR42_RS20025 0.36 +0.2 4,325,670 - RR42_RS20025 0.36 -1.0 4,325,670 - RR42_RS20025 0.36 -0.9 4,325,670 - RR42_RS20025 0.36 -0.6 4,325,670 - RR42_RS20025 0.36 -0.5 4,325,670 - RR42_RS20025 0.36 +1.2 4,325,675 + RR42_RS20025 0.36 -0.3 4,325,795 + RR42_RS20025 0.44 -1.2 4,325,801 + RR42_RS20025 0.44 +0.2 4,325,802 - RR42_RS20025 0.44 -0.9 4,325,885 + RR42_RS20025 0.50 -0.4 4,325,886 - RR42_RS20025 0.50 -0.6 4,325,886 - RR42_RS20025 0.50 -2.8 4,326,270 - RR42_RS20025 0.74 -0.8 4,326,324 - RR42_RS20025 0.77 +1.3 4,326,356 + RR42_RS20025 0.79 -0.8 4,326,356 + RR42_RS20025 0.79 -1.1 4,326,356 + RR42_RS20025 0.79 -0.3 4,326,357 - RR42_RS20025 0.79 -0.9 4,326,357 - RR42_RS20025 0.79 -0.5 4,326,357 - RR42_RS20025 0.79 -0.5 4,326,357 - RR42_RS20025 0.79 -0.2 4,326,491 + RR42_RS20025 0.87 -1.7 4,326,508 - RR42_RS20025 0.88 -0.6 4,326,729 + +0.4 4,327,082 - RR42_RS20030 0.16 +1.6 4,327,082 - RR42_RS20030 0.16 -0.8 4,327,409 - RR42_RS20030 0.44 -0.3 4,327,409 - RR42_RS20030 0.44 -0.6 4,327,409 - RR42_RS20030 0.44 -0.4 4,327,660 + RR42_RS20030 0.66 +1.3
Or see this region's nucleotide sequence