Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18530 and RR42_RS18535 are separated by 129 nucleotides RR42_RS18535 and RR42_RS18540 are separated by 88 nucleotides
RR42_RS18530: RR42_RS18530 - chloride channel protein EriC, at 4,008,711 to 4,010,183
_RS18530
RR42_RS18535: RR42_RS18535 - ABC transporter substrate-binding protein, at 4,010,313 to 4,011,290
_RS18535
RR42_RS18540: RR42_RS18540 - long-chain fatty acid--CoA ligase, at 4,011,379 to 4,012,884
_RS18540
Position (kb)
4010
4011
4012 Strain fitness (log2 ratio)
-2
-1
0
1 at 4009.402 kb on + strand, within RR42_RS18530 at 4009.402 kb on + strand, within RR42_RS18530 at 4009.402 kb on + strand, within RR42_RS18530 at 4009.403 kb on - strand, within RR42_RS18530 at 4009.403 kb on - strand, within RR42_RS18530 at 4009.535 kb on + strand, within RR42_RS18530 at 4010.012 kb on - strand, within RR42_RS18530 at 4010.012 kb on - strand, within RR42_RS18530 at 4010.016 kb on + strand, within RR42_RS18530 at 4010.017 kb on - strand, within RR42_RS18530 at 4010.017 kb on - strand, within RR42_RS18530 at 4010.033 kb on + strand, within RR42_RS18530 at 4010.033 kb on + strand, within RR42_RS18530 at 4010.034 kb on - strand, within RR42_RS18530 at 4010.034 kb on - strand, within RR42_RS18530 at 4010.034 kb on - strand, within RR42_RS18530 at 4010.255 kb on + strand at 4010.255 kb on + strand at 4010.256 kb on - strand at 4010.256 kb on - strand at 4010.256 kb on - strand at 4010.257 kb on + strand at 4010.257 kb on + strand at 4010.257 kb on + strand at 4010.258 kb on - strand at 4010.377 kb on + strand at 4010.377 kb on + strand at 4010.377 kb on + strand at 4010.378 kb on - strand at 4010.716 kb on - strand, within RR42_RS18535 at 4010.716 kb on - strand, within RR42_RS18535 at 4010.716 kb on - strand, within RR42_RS18535 at 4010.737 kb on + strand, within RR42_RS18535 at 4010.825 kb on - strand, within RR42_RS18535 at 4010.854 kb on + strand, within RR42_RS18535 at 4010.855 kb on - strand, within RR42_RS18535 at 4010.855 kb on - strand, within RR42_RS18535 at 4010.977 kb on + strand, within RR42_RS18535 at 4011.028 kb on + strand, within RR42_RS18535 at 4011.028 kb on + strand, within RR42_RS18535 at 4011.029 kb on - strand, within RR42_RS18535 at 4011.176 kb on + strand, within RR42_RS18535 at 4011.176 kb on + strand, within RR42_RS18535 at 4011.176 kb on + strand, within RR42_RS18535 at 4011.177 kb on - strand, within RR42_RS18535 at 4011.178 kb on + strand, within RR42_RS18535 at 4011.178 kb on + strand, within RR42_RS18535 at 4011.178 kb on + strand, within RR42_RS18535 at 4011.212 kb on - strand at 4011.284 kb on + strand at 4011.284 kb on + strand at 4011.285 kb on - strand at 4011.285 kb on - strand at 4011.296 kb on - strand at 4011.296 kb on - strand at 4011.296 kb on - strand at 4011.407 kb on + strand at 4011.408 kb on - strand at 4011.591 kb on - strand, within RR42_RS18540 at 4011.671 kb on + strand, within RR42_RS18540 at 4011.671 kb on + strand, within RR42_RS18540 at 4011.671 kb on + strand, within RR42_RS18540 at 4011.728 kb on + strand, within RR42_RS18540 at 4011.747 kb on - strand, within RR42_RS18540 at 4011.815 kb on + strand, within RR42_RS18540 at 4011.815 kb on + strand, within RR42_RS18540 at 4011.848 kb on + strand, within RR42_RS18540 at 4011.848 kb on + strand, within RR42_RS18540 at 4011.848 kb on + strand, within RR42_RS18540 at 4011.849 kb on - strand, within RR42_RS18540 at 4011.849 kb on - strand, within RR42_RS18540 at 4011.849 kb on - strand, within RR42_RS18540 at 4011.976 kb on - strand, within RR42_RS18540 at 4012.001 kb on + strand, within RR42_RS18540 at 4012.001 kb on + strand, within RR42_RS18540 at 4012.001 kb on + strand, within RR42_RS18540 at 4012.001 kb on + strand, within RR42_RS18540 at 4012.002 kb on - strand, within RR42_RS18540 at 4012.002 kb on - strand, within RR42_RS18540 at 4012.002 kb on - strand, within RR42_RS18540 at 4012.148 kb on + strand, within RR42_RS18540 at 4012.148 kb on + strand, within RR42_RS18540 at 4012.148 kb on + strand, within RR42_RS18540 at 4012.148 kb on + strand, within RR42_RS18540 at 4012.148 kb on + strand, within RR42_RS18540 at 4012.148 kb on + strand, within RR42_RS18540 at 4012.148 kb on + strand, within RR42_RS18540 at 4012.148 kb on + strand, within RR42_RS18540 at 4012.148 kb on + strand, within RR42_RS18540 at 4012.148 kb on + strand, within RR42_RS18540 at 4012.149 kb on - strand, within RR42_RS18540 at 4012.149 kb on - strand, within RR42_RS18540 at 4012.149 kb on - strand, within RR42_RS18540 at 4012.149 kb on - strand, within RR42_RS18540 at 4012.149 kb on - strand, within RR42_RS18540 at 4012.149 kb on - strand, within RR42_RS18540 at 4012.149 kb on - strand, within RR42_RS18540 at 4012.149 kb on - strand, within RR42_RS18540 at 4012.149 kb on - strand, within RR42_RS18540 at 4012.166 kb on + strand, within RR42_RS18540 at 4012.167 kb on - strand, within RR42_RS18540 at 4012.167 kb on - strand, within RR42_RS18540 at 4012.167 kb on - strand, within RR42_RS18540 at 4012.192 kb on + strand, within RR42_RS18540 at 4012.193 kb on - strand, within RR42_RS18540
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 4,009,402 + RR42_RS18530 0.47 +0.4 4,009,402 + RR42_RS18530 0.47 -0.5 4,009,402 + RR42_RS18530 0.47 +0.4 4,009,403 - RR42_RS18530 0.47 +0.1 4,009,403 - RR42_RS18530 0.47 -2.7 4,009,535 + RR42_RS18530 0.56 +0.3 4,010,012 - RR42_RS18530 0.88 +0.3 4,010,012 - RR42_RS18530 0.88 +1.1 4,010,016 + RR42_RS18530 0.89 -0.1 4,010,017 - RR42_RS18530 0.89 -0.5 4,010,017 - RR42_RS18530 0.89 -0.7 4,010,033 + RR42_RS18530 0.90 +0.2 4,010,033 + RR42_RS18530 0.90 -1.9 4,010,034 - RR42_RS18530 0.90 +0.1 4,010,034 - RR42_RS18530 0.90 +1.0 4,010,034 - RR42_RS18530 0.90 +0.4 4,010,255 + +0.0 4,010,255 + +0.4 4,010,256 - -0.5 4,010,256 - -0.1 4,010,256 - -0.1 4,010,257 + +0.7 4,010,257 + +0.7 4,010,257 + -0.6 4,010,258 - -0.7 4,010,377 + -0.4 4,010,377 + +0.1 4,010,377 + -0.8 4,010,378 - +1.7 4,010,716 - RR42_RS18535 0.41 -0.1 4,010,716 - RR42_RS18535 0.41 -1.7 4,010,716 - RR42_RS18535 0.41 +0.2 4,010,737 + RR42_RS18535 0.43 -0.3 4,010,825 - RR42_RS18535 0.52 -0.1 4,010,854 + RR42_RS18535 0.55 -0.2 4,010,855 - RR42_RS18535 0.55 +0.0 4,010,855 - RR42_RS18535 0.55 -0.9 4,010,977 + RR42_RS18535 0.68 -0.9 4,011,028 + RR42_RS18535 0.73 -0.3 4,011,028 + RR42_RS18535 0.73 -0.4 4,011,029 - RR42_RS18535 0.73 -0.2 4,011,176 + RR42_RS18535 0.88 +0.1 4,011,176 + RR42_RS18535 0.88 +0.4 4,011,176 + RR42_RS18535 0.88 +0.1 4,011,177 - RR42_RS18535 0.88 +1.7 4,011,178 + RR42_RS18535 0.88 +1.7 4,011,178 + RR42_RS18535 0.88 -0.7 4,011,178 + RR42_RS18535 0.88 +0.7 4,011,212 - -0.9 4,011,284 + -0.1 4,011,284 + -0.0 4,011,285 - +0.0 4,011,285 - -0.8 4,011,296 - -0.1 4,011,296 - +0.9 4,011,296 - +0.6 4,011,407 + -0.6 4,011,408 - +0.6 4,011,591 - RR42_RS18540 0.14 -0.6 4,011,671 + RR42_RS18540 0.19 -0.7 4,011,671 + RR42_RS18540 0.19 +0.2 4,011,671 + RR42_RS18540 0.19 -0.8 4,011,728 + RR42_RS18540 0.23 +0.9 4,011,747 - RR42_RS18540 0.24 -0.5 4,011,815 + RR42_RS18540 0.29 -0.3 4,011,815 + RR42_RS18540 0.29 +0.3 4,011,848 + RR42_RS18540 0.31 -0.3 4,011,848 + RR42_RS18540 0.31 +0.8 4,011,848 + RR42_RS18540 0.31 +0.3 4,011,849 - RR42_RS18540 0.31 +1.4 4,011,849 - RR42_RS18540 0.31 +0.1 4,011,849 - RR42_RS18540 0.31 +0.3 4,011,976 - RR42_RS18540 0.40 -0.0 4,012,001 + RR42_RS18540 0.41 -1.1 4,012,001 + RR42_RS18540 0.41 +0.6 4,012,001 + RR42_RS18540 0.41 -0.5 4,012,001 + RR42_RS18540 0.41 -0.1 4,012,002 - RR42_RS18540 0.41 +0.2 4,012,002 - RR42_RS18540 0.41 +1.1 4,012,002 - RR42_RS18540 0.41 -1.0 4,012,148 + RR42_RS18540 0.51 -0.6 4,012,148 + RR42_RS18540 0.51 +0.8 4,012,148 + RR42_RS18540 0.51 +0.3 4,012,148 + RR42_RS18540 0.51 -0.7 4,012,148 + RR42_RS18540 0.51 -0.4 4,012,148 + RR42_RS18540 0.51 -0.6 4,012,148 + RR42_RS18540 0.51 -1.8 4,012,148 + RR42_RS18540 0.51 -0.9 4,012,148 + RR42_RS18540 0.51 -0.8 4,012,148 + RR42_RS18540 0.51 -1.0 4,012,149 - RR42_RS18540 0.51 +0.5 4,012,149 - RR42_RS18540 0.51 -1.6 4,012,149 - RR42_RS18540 0.51 -2.0 4,012,149 - RR42_RS18540 0.51 +0.8 4,012,149 - RR42_RS18540 0.51 -0.0 4,012,149 - RR42_RS18540 0.51 -0.3 4,012,149 - RR42_RS18540 0.51 +0.6 4,012,149 - RR42_RS18540 0.51 -0.4 4,012,149 - RR42_RS18540 0.51 -0.2 4,012,166 + RR42_RS18540 0.52 -1.6 4,012,167 - RR42_RS18540 0.52 +0.7 4,012,167 - RR42_RS18540 0.52 +0.9 4,012,167 - RR42_RS18540 0.52 -0.4 4,012,192 + RR42_RS18540 0.54 -0.7 4,012,193 - RR42_RS18540 0.54 +0.5
Or see this region's nucleotide sequence