Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18330 and RR42_RS18335 are separated by 81 nucleotides RR42_RS18335 and RR42_RS18340 are separated by 26 nucleotides
RR42_RS18330: RR42_RS18330 - sulfonate ABC transporter permease, at 3,969,386 to 3,971,140
_RS18330
RR42_RS18335: RR42_RS18335 - nitrate ABC transporter ATP-binding protein, at 3,971,222 to 3,972,568
_RS18335
RR42_RS18340: RR42_RS18340 - membrane protein, at 3,972,595 to 3,975,105
_RS18340
Position (kb)
3971
3972
3973 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3970.458 kb on - strand, within RR42_RS18330 at 3970.763 kb on + strand, within RR42_RS18330 at 3970.763 kb on + strand, within RR42_RS18330 at 3970.763 kb on + strand, within RR42_RS18330 at 3970.764 kb on - strand, within RR42_RS18330 at 3970.940 kb on - strand, within RR42_RS18330 at 3970.953 kb on - strand, within RR42_RS18330 at 3971.000 kb on + strand at 3971.000 kb on + strand at 3971.000 kb on + strand at 3971.001 kb on - strand at 3971.001 kb on - strand at 3971.001 kb on - strand at 3971.001 kb on - strand at 3971.001 kb on - strand at 3971.001 kb on - strand at 3971.001 kb on - strand at 3971.001 kb on - strand at 3971.001 kb on - strand at 3971.001 kb on - strand at 3971.001 kb on - strand at 3971.002 kb on + strand at 3971.109 kb on - strand at 3971.148 kb on + strand at 3971.149 kb on - strand at 3971.149 kb on - strand at 3971.168 kb on + strand at 3971.179 kb on + strand at 3971.179 kb on + strand at 3971.256 kb on + strand at 3971.257 kb on - strand at 3971.259 kb on + strand at 3971.259 kb on + strand at 3971.259 kb on + strand at 3971.260 kb on - strand at 3971.260 kb on - strand at 3971.813 kb on - strand, within RR42_RS18335 at 3971.813 kb on - strand, within RR42_RS18335 at 3971.813 kb on - strand, within RR42_RS18335 at 3971.813 kb on - strand, within RR42_RS18335 at 3971.985 kb on - strand, within RR42_RS18335 at 3971.985 kb on - strand, within RR42_RS18335 at 3971.985 kb on - strand, within RR42_RS18335 at 3971.985 kb on - strand, within RR42_RS18335 at 3971.985 kb on - strand, within RR42_RS18335 at 3972.272 kb on + strand, within RR42_RS18335 at 3972.273 kb on - strand, within RR42_RS18335 at 3972.273 kb on - strand, within RR42_RS18335 at 3972.488 kb on + strand at 3972.494 kb on + strand at 3972.494 kb on + strand at 3972.495 kb on - strand at 3972.500 kb on + strand at 3972.501 kb on - strand at 3972.501 kb on - strand at 3972.501 kb on - strand at 3972.501 kb on - strand at 3972.596 kb on + strand at 3973.224 kb on - strand, within RR42_RS18340 at 3973.274 kb on + strand, within RR42_RS18340 at 3973.275 kb on - strand, within RR42_RS18340 at 3973.275 kb on - strand, within RR42_RS18340 at 3973.275 kb on - strand, within RR42_RS18340 at 3973.275 kb on - strand, within RR42_RS18340 at 3973.326 kb on + strand, within RR42_RS18340 at 3973.326 kb on + strand, within RR42_RS18340 at 3973.326 kb on + strand, within RR42_RS18340 at 3973.327 kb on - strand, within RR42_RS18340 at 3973.455 kb on + strand, within RR42_RS18340 at 3973.457 kb on + strand, within RR42_RS18340 at 3973.509 kb on - strand, within RR42_RS18340
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 3,970,458 - RR42_RS18330 0.61 -0.6 3,970,763 + RR42_RS18330 0.78 -0.3 3,970,763 + RR42_RS18330 0.78 +0.1 3,970,763 + RR42_RS18330 0.78 +0.2 3,970,764 - RR42_RS18330 0.79 -0.7 3,970,940 - RR42_RS18330 0.89 +0.4 3,970,953 - RR42_RS18330 0.89 +0.4 3,971,000 + +0.3 3,971,000 + -0.4 3,971,000 + +0.2 3,971,001 - -1.1 3,971,001 - -0.3 3,971,001 - -0.8 3,971,001 - -0.1 3,971,001 - +0.3 3,971,001 - -1.6 3,971,001 - -0.8 3,971,001 - -0.2 3,971,001 - -0.5 3,971,001 - -1.9 3,971,001 - +0.3 3,971,002 + -1.1 3,971,109 - +0.8 3,971,148 + -0.3 3,971,149 - -1.7 3,971,149 - +0.6 3,971,168 + +0.1 3,971,179 + -0.6 3,971,179 + -0.7 3,971,256 + -0.3 3,971,257 - +0.6 3,971,259 + -0.6 3,971,259 + -0.3 3,971,259 + -1.2 3,971,260 - +1.8 3,971,260 - +0.1 3,971,813 - RR42_RS18335 0.44 -1.0 3,971,813 - RR42_RS18335 0.44 -0.2 3,971,813 - RR42_RS18335 0.44 +0.4 3,971,813 - RR42_RS18335 0.44 +0.7 3,971,985 - RR42_RS18335 0.57 -0.9 3,971,985 - RR42_RS18335 0.57 +0.9 3,971,985 - RR42_RS18335 0.57 +0.8 3,971,985 - RR42_RS18335 0.57 +1.0 3,971,985 - RR42_RS18335 0.57 +0.5 3,972,272 + RR42_RS18335 0.78 +0.9 3,972,273 - RR42_RS18335 0.78 +0.6 3,972,273 - RR42_RS18335 0.78 +0.4 3,972,488 + +0.8 3,972,494 + +0.0 3,972,494 + +0.0 3,972,495 - -0.5 3,972,500 + +1.2 3,972,501 - -0.2 3,972,501 - -2.0 3,972,501 - +0.3 3,972,501 - +0.5 3,972,596 + +0.1 3,973,224 - RR42_RS18340 0.25 -3.4 3,973,274 + RR42_RS18340 0.27 -0.6 3,973,275 - RR42_RS18340 0.27 -0.4 3,973,275 - RR42_RS18340 0.27 +0.1 3,973,275 - RR42_RS18340 0.27 -1.1 3,973,275 - RR42_RS18340 0.27 -0.0 3,973,326 + RR42_RS18340 0.29 -0.6 3,973,326 + RR42_RS18340 0.29 -0.4 3,973,326 + RR42_RS18340 0.29 +0.8 3,973,327 - RR42_RS18340 0.29 +0.8 3,973,455 + RR42_RS18340 0.34 -0.2 3,973,457 + RR42_RS18340 0.34 +1.1 3,973,509 - RR42_RS18340 0.36 +0.0
Or see this region's nucleotide sequence