Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS18160 and RR42_RS18165 are separated by 51 nucleotides RR42_RS18165 and RR42_RS18170 are separated by 89 nucleotides
RR42_RS18160: RR42_RS18160 - acyl-CoA synthetase, at 3,929,782 to 3,931,512
_RS18160
RR42_RS18165: RR42_RS18165 - carbamoyl-phosphate synthase large subunit, at 3,931,564 to 3,934,902
_RS18165
RR42_RS18170: RR42_RS18170 - amino acid ABC transporter substrate-binding protein, at 3,934,992 to 3,936,266
_RS18170
Position (kb)
3931
3932
3933
3934
3935 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3930.767 kb on - strand, within RR42_RS18160 at 3930.797 kb on + strand, within RR42_RS18160 at 3930.920 kb on - strand, within RR42_RS18160 at 3930.991 kb on + strand, within RR42_RS18160 at 3931.171 kb on + strand, within RR42_RS18160 at 3931.172 kb on - strand, within RR42_RS18160 at 3931.172 kb on - strand, within RR42_RS18160 at 3931.411 kb on + strand at 3931.411 kb on + strand at 3931.412 kb on - strand at 3931.412 kb on - strand at 3931.412 kb on - strand at 3931.431 kb on + strand at 3931.431 kb on + strand at 3931.431 kb on + strand at 3931.431 kb on + strand at 3931.432 kb on - strand at 3931.432 kb on - strand at 3931.432 kb on - strand at 3931.432 kb on - strand at 3931.432 kb on - strand at 3931.432 kb on - strand at 3931.581 kb on - strand at 3931.581 kb on - strand at 3931.660 kb on + strand at 3931.661 kb on - strand at 3931.662 kb on + strand at 3931.760 kb on + strand at 3932.116 kb on + strand, within RR42_RS18165 at 3932.116 kb on + strand, within RR42_RS18165 at 3932.116 kb on + strand, within RR42_RS18165 at 3932.117 kb on - strand, within RR42_RS18165 at 3932.167 kb on + strand, within RR42_RS18165 at 3932.168 kb on - strand, within RR42_RS18165 at 3932.677 kb on + strand, within RR42_RS18165 at 3932.678 kb on - strand, within RR42_RS18165 at 3932.730 kb on + strand, within RR42_RS18165 at 3933.670 kb on + strand, within RR42_RS18165 at 3933.671 kb on - strand, within RR42_RS18165 at 3933.671 kb on - strand, within RR42_RS18165 at 3933.894 kb on - strand, within RR42_RS18165 at 3933.894 kb on - strand, within RR42_RS18165 at 3933.894 kb on - strand, within RR42_RS18165 at 3933.894 kb on - strand, within RR42_RS18165 at 3933.894 kb on - strand, within RR42_RS18165 at 3934.085 kb on - strand, within RR42_RS18165 at 3934.085 kb on - strand, within RR42_RS18165 at 3934.094 kb on - strand, within RR42_RS18165 at 3934.177 kb on + strand, within RR42_RS18165 at 3934.177 kb on + strand, within RR42_RS18165 at 3934.178 kb on - strand, within RR42_RS18165 at 3934.178 kb on - strand, within RR42_RS18165 at 3934.752 kb on + strand at 3934.756 kb on - strand at 3935.041 kb on + strand at 3935.232 kb on + strand, within RR42_RS18170 at 3935.233 kb on - strand, within RR42_RS18170 at 3935.233 kb on - strand, within RR42_RS18170 at 3935.406 kb on + strand, within RR42_RS18170 at 3935.407 kb on - strand, within RR42_RS18170 at 3935.538 kb on + strand, within RR42_RS18170 at 3935.538 kb on + strand, within RR42_RS18170 at 3935.539 kb on - strand, within RR42_RS18170 at 3935.539 kb on - strand, within RR42_RS18170 at 3935.539 kb on - strand, within RR42_RS18170 at 3935.545 kb on + strand, within RR42_RS18170 at 3935.545 kb on + strand, within RR42_RS18170 at 3935.545 kb on + strand, within RR42_RS18170 at 3935.556 kb on + strand, within RR42_RS18170 at 3935.556 kb on + strand, within RR42_RS18170 at 3935.556 kb on + strand, within RR42_RS18170 at 3935.557 kb on - strand, within RR42_RS18170 at 3935.676 kb on + strand, within RR42_RS18170 at 3935.677 kb on - strand, within RR42_RS18170 at 3935.787 kb on + strand, within RR42_RS18170 at 3935.799 kb on - strand, within RR42_RS18170
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 3,930,767 - RR42_RS18160 0.57 -0.9 3,930,797 + RR42_RS18160 0.59 +0.5 3,930,920 - RR42_RS18160 0.66 -0.4 3,930,991 + RR42_RS18160 0.70 -0.8 3,931,171 + RR42_RS18160 0.80 -0.2 3,931,172 - RR42_RS18160 0.80 +0.2 3,931,172 - RR42_RS18160 0.80 -0.1 3,931,411 + +0.5 3,931,411 + -0.1 3,931,412 - +1.0 3,931,412 - -0.3 3,931,412 - -0.4 3,931,431 + +0.3 3,931,431 + -0.0 3,931,431 + -3.0 3,931,431 + +0.1 3,931,432 - -0.2 3,931,432 - +0.1 3,931,432 - +0.3 3,931,432 - -0.3 3,931,432 - -0.7 3,931,432 - -1.2 3,931,581 - +0.7 3,931,581 - -0.2 3,931,660 + +0.1 3,931,661 - +0.4 3,931,662 + -0.1 3,931,760 + -2.9 3,932,116 + RR42_RS18165 0.17 +2.0 3,932,116 + RR42_RS18165 0.17 -0.4 3,932,116 + RR42_RS18165 0.17 -0.0 3,932,117 - RR42_RS18165 0.17 -1.2 3,932,167 + RR42_RS18165 0.18 +0.6 3,932,168 - RR42_RS18165 0.18 -0.0 3,932,677 + RR42_RS18165 0.33 -1.1 3,932,678 - RR42_RS18165 0.33 -0.6 3,932,730 + RR42_RS18165 0.35 -0.5 3,933,670 + RR42_RS18165 0.63 -0.2 3,933,671 - RR42_RS18165 0.63 +0.2 3,933,671 - RR42_RS18165 0.63 -1.3 3,933,894 - RR42_RS18165 0.70 -0.2 3,933,894 - RR42_RS18165 0.70 -0.3 3,933,894 - RR42_RS18165 0.70 +0.2 3,933,894 - RR42_RS18165 0.70 -0.6 3,933,894 - RR42_RS18165 0.70 -0.1 3,934,085 - RR42_RS18165 0.76 -0.5 3,934,085 - RR42_RS18165 0.76 +0.8 3,934,094 - RR42_RS18165 0.76 +0.2 3,934,177 + RR42_RS18165 0.78 -0.2 3,934,177 + RR42_RS18165 0.78 +0.2 3,934,178 - RR42_RS18165 0.78 -0.6 3,934,178 - RR42_RS18165 0.78 +0.4 3,934,752 + +0.4 3,934,756 - +0.5 3,935,041 + -0.5 3,935,232 + RR42_RS18170 0.19 +1.6 3,935,233 - RR42_RS18170 0.19 -0.2 3,935,233 - RR42_RS18170 0.19 -1.1 3,935,406 + RR42_RS18170 0.32 +0.0 3,935,407 - RR42_RS18170 0.33 -0.3 3,935,538 + RR42_RS18170 0.43 -0.3 3,935,538 + RR42_RS18170 0.43 +0.3 3,935,539 - RR42_RS18170 0.43 -1.0 3,935,539 - RR42_RS18170 0.43 -1.5 3,935,539 - RR42_RS18170 0.43 +0.9 3,935,545 + RR42_RS18170 0.43 -0.1 3,935,545 + RR42_RS18170 0.43 +0.7 3,935,545 + RR42_RS18170 0.43 +0.3 3,935,556 + RR42_RS18170 0.44 +0.3 3,935,556 + RR42_RS18170 0.44 +0.7 3,935,556 + RR42_RS18170 0.44 -1.5 3,935,557 - RR42_RS18170 0.44 -0.2 3,935,676 + RR42_RS18170 0.54 -1.0 3,935,677 - RR42_RS18170 0.54 -0.8 3,935,787 + RR42_RS18170 0.62 -1.0 3,935,799 - RR42_RS18170 0.63 -1.1
Or see this region's nucleotide sequence