Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13945 and RR42_RS13950 are separated by 282 nucleotides RR42_RS13950 and RR42_RS13955 are separated by 197 nucleotides RR42_RS13955 and RR42_RS13960 are separated by 48 nucleotides RR42_RS13960 and RR42_RS13965 are separated by 124 nucleotides
RR42_RS13945: RR42_RS13945 - hypothetical protein, at 3,005,664 to 3,005,921
_RS13945
RR42_RS13950: RR42_RS13950 - hypothetical protein, at 3,006,204 to 3,006,530
_RS13950
RR42_RS13955: RR42_RS13955 - tRNA-Leu, at 3,006,728 to 3,006,814
_RS13955
RR42_RS13960: RR42_RS13960 - hypothetical protein, at 3,006,863 to 3,007,966
_RS13960
RR42_RS13965: RR42_RS13965 - LysR family transcriptional regulator, at 3,008,091 to 3,008,996
_RS13965
Position (kb)
3006
3007
3008 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3005.942 kb on + strand at 3005.942 kb on + strand at 3005.943 kb on - strand at 3005.943 kb on - strand at 3005.943 kb on - strand at 3006.015 kb on + strand at 3006.015 kb on + strand at 3006.016 kb on - strand at 3006.085 kb on + strand at 3006.086 kb on - strand at 3006.089 kb on + strand at 3006.089 kb on + strand at 3006.089 kb on + strand at 3006.090 kb on - strand at 3006.090 kb on - strand at 3006.090 kb on - strand at 3006.205 kb on + strand at 3006.239 kb on - strand, within RR42_RS13950 at 3006.250 kb on + strand, within RR42_RS13950 at 3006.251 kb on - strand, within RR42_RS13950 at 3006.251 kb on - strand, within RR42_RS13950 at 3006.251 kb on - strand, within RR42_RS13950 at 3006.265 kb on + strand, within RR42_RS13950 at 3006.266 kb on - strand, within RR42_RS13950 at 3006.268 kb on + strand, within RR42_RS13950 at 3006.301 kb on + strand, within RR42_RS13950 at 3006.301 kb on + strand, within RR42_RS13950 at 3006.302 kb on - strand, within RR42_RS13950 at 3006.304 kb on + strand, within RR42_RS13950 at 3006.305 kb on - strand, within RR42_RS13950 at 3006.305 kb on - strand, within RR42_RS13950 at 3006.343 kb on + strand, within RR42_RS13950 at 3006.343 kb on + strand, within RR42_RS13950 at 3006.344 kb on - strand, within RR42_RS13950 at 3006.344 kb on - strand, within RR42_RS13950 at 3006.346 kb on + strand, within RR42_RS13950 at 3006.347 kb on - strand, within RR42_RS13950 at 3006.347 kb on - strand, within RR42_RS13950 at 3006.386 kb on + strand, within RR42_RS13950 at 3006.386 kb on + strand, within RR42_RS13950 at 3006.386 kb on + strand, within RR42_RS13950 at 3006.386 kb on + strand, within RR42_RS13950 at 3006.386 kb on + strand, within RR42_RS13950 at 3006.387 kb on - strand, within RR42_RS13950 at 3006.387 kb on - strand, within RR42_RS13950 at 3006.387 kb on - strand, within RR42_RS13950 at 3006.387 kb on - strand, within RR42_RS13950 at 3006.387 kb on - strand, within RR42_RS13950 at 3006.388 kb on + strand, within RR42_RS13950 at 3006.389 kb on - strand, within RR42_RS13950 at 3006.389 kb on - strand, within RR42_RS13950 at 3006.389 kb on - strand, within RR42_RS13950 at 3006.389 kb on - strand, within RR42_RS13950 at 3006.406 kb on + strand, within RR42_RS13950 at 3006.406 kb on + strand, within RR42_RS13950 at 3006.406 kb on + strand, within RR42_RS13950 at 3006.537 kb on - strand at 3006.537 kb on - strand at 3006.537 kb on - strand at 3006.537 kb on - strand at 3006.554 kb on + strand at 3006.568 kb on + strand at 3006.568 kb on + strand at 3006.569 kb on - strand at 3006.573 kb on + strand at 3006.573 kb on + strand at 3006.574 kb on - strand at 3006.574 kb on - strand at 3006.610 kb on + strand at 3006.610 kb on + strand at 3006.611 kb on - strand at 3006.618 kb on + strand at 3006.641 kb on + strand at 3006.641 kb on + strand at 3006.641 kb on + strand at 3006.641 kb on + strand at 3006.641 kb on + strand at 3006.641 kb on + strand at 3006.641 kb on + strand at 3006.641 kb on + strand at 3006.641 kb on + strand at 3006.641 kb on + strand at 3006.642 kb on - strand at 3006.642 kb on - strand at 3006.673 kb on - strand at 3006.820 kb on + strand at 3006.820 kb on + strand at 3006.820 kb on + strand at 3006.821 kb on - strand at 3006.821 kb on - strand at 3006.821 kb on - strand at 3007.015 kb on + strand, within RR42_RS13960 at 3007.338 kb on + strand, within RR42_RS13960 at 3007.338 kb on + strand, within RR42_RS13960 at 3007.401 kb on + strand, within RR42_RS13960 at 3007.407 kb on + strand, within RR42_RS13960 at 3007.407 kb on + strand, within RR42_RS13960 at 3007.408 kb on - strand, within RR42_RS13960 at 3007.408 kb on - strand, within RR42_RS13960 at 3007.408 kb on - strand, within RR42_RS13960 at 3007.436 kb on + strand, within RR42_RS13960 at 3007.436 kb on + strand, within RR42_RS13960 at 3007.436 kb on + strand, within RR42_RS13960 at 3007.437 kb on - strand, within RR42_RS13960 at 3007.520 kb on + strand, within RR42_RS13960 at 3007.520 kb on + strand, within RR42_RS13960 at 3007.521 kb on - strand, within RR42_RS13960 at 3007.521 kb on - strand, within RR42_RS13960 at 3007.563 kb on - strand, within RR42_RS13960 at 3007.687 kb on - strand, within RR42_RS13960 at 3007.687 kb on - strand, within RR42_RS13960 at 3007.687 kb on - strand, within RR42_RS13960 at 3007.687 kb on - strand, within RR42_RS13960 at 3007.771 kb on + strand, within RR42_RS13960 at 3007.771 kb on + strand, within RR42_RS13960 at 3007.771 kb on + strand, within RR42_RS13960 at 3007.771 kb on + strand, within RR42_RS13960 at 3007.771 kb on + strand, within RR42_RS13960 at 3008.009 kb on + strand at 3008.009 kb on + strand at 3008.010 kb on - strand at 3008.010 kb on - strand at 3008.016 kb on + strand at 3008.029 kb on + strand at 3008.029 kb on + strand at 3008.029 kb on + strand at 3008.030 kb on - strand at 3008.030 kb on - strand at 3008.033 kb on + strand at 3008.033 kb on + strand at 3008.033 kb on + strand at 3008.033 kb on + strand at 3008.033 kb on + strand at 3008.033 kb on + strand at 3008.033 kb on + strand at 3008.033 kb on + strand at 3008.033 kb on + strand at 3008.034 kb on - strand at 3008.034 kb on - strand at 3008.034 kb on - strand at 3008.034 kb on - strand at 3008.034 kb on - strand at 3008.034 kb on - strand at 3008.034 kb on - strand at 3008.034 kb on - strand at 3008.043 kb on - strand at 3008.070 kb on + strand at 3008.399 kb on + strand, within RR42_RS13965 at 3008.409 kb on + strand, within RR42_RS13965 at 3008.409 kb on + strand, within RR42_RS13965 at 3008.409 kb on + strand, within RR42_RS13965 at 3008.409 kb on + strand, within RR42_RS13965 at 3008.409 kb on + strand, within RR42_RS13965 at 3008.409 kb on + strand, within RR42_RS13965 at 3008.409 kb on + strand, within RR42_RS13965 at 3008.409 kb on + strand, within RR42_RS13965 at 3008.411 kb on + strand, within RR42_RS13965 at 3008.411 kb on + strand, within RR42_RS13965 at 3008.411 kb on + strand, within RR42_RS13965 at 3008.634 kb on + strand, within RR42_RS13965 at 3008.929 kb on - strand at 3008.929 kb on - strand at 3008.929 kb on - strand at 3008.934 kb on - strand at 3008.934 kb on - strand at 3008.934 kb on - strand at 3008.962 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 3,005,942 + +0.6 3,005,942 + +0.3 3,005,943 - +0.4 3,005,943 - +0.6 3,005,943 - +0.4 3,006,015 + +0.2 3,006,015 + +0.2 3,006,016 - -2.8 3,006,085 + +0.8 3,006,086 - -0.9 3,006,089 + +0.3 3,006,089 + +0.8 3,006,089 + +0.6 3,006,090 - +0.3 3,006,090 - -0.7 3,006,090 - +1.6 3,006,205 + -0.3 3,006,239 - RR42_RS13950 0.11 +0.3 3,006,250 + RR42_RS13950 0.14 +0.3 3,006,251 - RR42_RS13950 0.14 -1.3 3,006,251 - RR42_RS13950 0.14 +0.3 3,006,251 - RR42_RS13950 0.14 +0.3 3,006,265 + RR42_RS13950 0.19 -0.9 3,006,266 - RR42_RS13950 0.19 +0.3 3,006,268 + RR42_RS13950 0.20 -0.4 3,006,301 + RR42_RS13950 0.30 +0.7 3,006,301 + RR42_RS13950 0.30 +0.9 3,006,302 - RR42_RS13950 0.30 -1.6 3,006,304 + RR42_RS13950 0.31 -0.9 3,006,305 - RR42_RS13950 0.31 -0.5 3,006,305 - RR42_RS13950 0.31 -0.2 3,006,343 + RR42_RS13950 0.43 +0.3 3,006,343 + RR42_RS13950 0.43 +0.3 3,006,344 - RR42_RS13950 0.43 +0.1 3,006,344 - RR42_RS13950 0.43 +0.3 3,006,346 + RR42_RS13950 0.43 +0.2 3,006,347 - RR42_RS13950 0.44 -0.2 3,006,347 - RR42_RS13950 0.44 +1.3 3,006,386 + RR42_RS13950 0.56 +0.1 3,006,386 + RR42_RS13950 0.56 -0.2 3,006,386 + RR42_RS13950 0.56 -0.0 3,006,386 + RR42_RS13950 0.56 -0.7 3,006,386 + RR42_RS13950 0.56 +0.1 3,006,387 - RR42_RS13950 0.56 -0.1 3,006,387 - RR42_RS13950 0.56 -0.2 3,006,387 - RR42_RS13950 0.56 +1.9 3,006,387 - RR42_RS13950 0.56 -0.3 3,006,387 - RR42_RS13950 0.56 +0.3 3,006,388 + RR42_RS13950 0.56 +0.3 3,006,389 - RR42_RS13950 0.57 -0.1 3,006,389 - RR42_RS13950 0.57 +0.2 3,006,389 - RR42_RS13950 0.57 -0.5 3,006,389 - RR42_RS13950 0.57 +0.7 3,006,406 + RR42_RS13950 0.62 -0.0 3,006,406 + RR42_RS13950 0.62 -2.7 3,006,406 + RR42_RS13950 0.62 -0.7 3,006,537 - +0.1 3,006,537 - +0.7 3,006,537 - -0.5 3,006,537 - -0.5 3,006,554 + +0.2 3,006,568 + +0.4 3,006,568 + -0.1 3,006,569 - +1.0 3,006,573 + +0.2 3,006,573 + +2.1 3,006,574 - +0.4 3,006,574 - -0.2 3,006,610 + +0.2 3,006,610 + -0.7 3,006,611 - -0.3 3,006,618 + -0.2 3,006,641 + +0.2 3,006,641 + +0.2 3,006,641 + +0.1 3,006,641 + +0.0 3,006,641 + +0.3 3,006,641 + -0.7 3,006,641 + -0.5 3,006,641 + -1.1 3,006,641 + +0.2 3,006,641 + +0.5 3,006,642 - +0.2 3,006,642 - -0.3 3,006,673 - -0.4 3,006,820 + +0.2 3,006,820 + +0.8 3,006,820 + +0.4 3,006,821 - -0.8 3,006,821 - -0.4 3,006,821 - +0.6 3,007,015 + RR42_RS13960 0.14 -0.3 3,007,338 + RR42_RS13960 0.43 -0.7 3,007,338 + RR42_RS13960 0.43 -0.3 3,007,401 + RR42_RS13960 0.49 +0.3 3,007,407 + RR42_RS13960 0.49 +0.4 3,007,407 + RR42_RS13960 0.49 +0.9 3,007,408 - RR42_RS13960 0.49 +0.3 3,007,408 - RR42_RS13960 0.49 -0.2 3,007,408 - RR42_RS13960 0.49 -0.3 3,007,436 + RR42_RS13960 0.52 +0.2 3,007,436 + RR42_RS13960 0.52 -1.9 3,007,436 + RR42_RS13960 0.52 -0.3 3,007,437 - RR42_RS13960 0.52 +0.4 3,007,520 + RR42_RS13960 0.60 +0.3 3,007,520 + RR42_RS13960 0.60 -0.5 3,007,521 - RR42_RS13960 0.60 +0.9 3,007,521 - RR42_RS13960 0.60 +1.1 3,007,563 - RR42_RS13960 0.63 +1.1 3,007,687 - RR42_RS13960 0.75 -0.1 3,007,687 - RR42_RS13960 0.75 -0.2 3,007,687 - RR42_RS13960 0.75 -1.2 3,007,687 - RR42_RS13960 0.75 +1.6 3,007,771 + RR42_RS13960 0.82 +0.9 3,007,771 + RR42_RS13960 0.82 +1.1 3,007,771 + RR42_RS13960 0.82 +0.4 3,007,771 + RR42_RS13960 0.82 -0.1 3,007,771 + RR42_RS13960 0.82 -0.4 3,008,009 + -0.2 3,008,009 + +0.1 3,008,010 - -0.7 3,008,010 - +0.7 3,008,016 + +0.3 3,008,029 + +0.8 3,008,029 + +0.1 3,008,029 + +0.5 3,008,030 - -0.5 3,008,030 - +0.7 3,008,033 + -0.3 3,008,033 + -0.2 3,008,033 + -0.8 3,008,033 + -0.1 3,008,033 + +0.5 3,008,033 + +0.2 3,008,033 + +0.1 3,008,033 + -2.1 3,008,033 + -0.5 3,008,034 - +0.7 3,008,034 - -0.7 3,008,034 - -0.9 3,008,034 - -0.2 3,008,034 - +0.1 3,008,034 - +0.8 3,008,034 - -0.9 3,008,034 - -0.1 3,008,043 - +0.5 3,008,070 + -0.8 3,008,399 + RR42_RS13965 0.34 +0.4 3,008,409 + RR42_RS13965 0.35 -0.2 3,008,409 + RR42_RS13965 0.35 +0.3 3,008,409 + RR42_RS13965 0.35 +0.2 3,008,409 + RR42_RS13965 0.35 +0.7 3,008,409 + RR42_RS13965 0.35 +0.0 3,008,409 + RR42_RS13965 0.35 -1.0 3,008,409 + RR42_RS13965 0.35 +0.1 3,008,409 + RR42_RS13965 0.35 +0.1 3,008,411 + RR42_RS13965 0.35 -0.6 3,008,411 + RR42_RS13965 0.35 +0.2 3,008,411 + RR42_RS13965 0.35 +0.3 3,008,634 + RR42_RS13965 0.60 +0.2 3,008,929 - +0.2 3,008,929 - -0.9 3,008,929 - +0.6 3,008,934 - -0.9 3,008,934 - +0.4 3,008,934 - -0.5 3,008,962 - -0.4
Or see this region's nucleotide sequence