Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13240 and RR42_RS13245 are separated by 194 nucleotides RR42_RS13245 and RR42_RS13250 are separated by 233 nucleotides
RR42_RS13240: RR42_RS13240 - mannosyltransferase, at 2,851,261 to 2,852,073
_RS13240
RR42_RS13245: RR42_RS13245 - ABC transporter substrate-binding protein, at 2,852,268 to 2,853,314
_RS13245
RR42_RS13250: RR42_RS13250 - LacI family transcriptional regulator, at 2,853,548 to 2,854,498
_RS13250
Position (kb)
2852
2853
2854 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2851.495 kb on + strand, within RR42_RS13240 at 2851.496 kb on - strand, within RR42_RS13240 at 2851.609 kb on + strand, within RR42_RS13240 at 2851.610 kb on - strand, within RR42_RS13240 at 2851.610 kb on - strand, within RR42_RS13240 at 2851.820 kb on + strand, within RR42_RS13240 at 2851.821 kb on - strand, within RR42_RS13240 at 2851.867 kb on + strand, within RR42_RS13240 at 2851.913 kb on + strand, within RR42_RS13240 at 2851.998 kb on + strand at 2852.000 kb on + strand at 2852.001 kb on - strand at 2852.011 kb on - strand at 2852.145 kb on + strand at 2852.148 kb on + strand at 2852.149 kb on - strand at 2852.269 kb on + strand at 2852.269 kb on + strand at 2852.269 kb on + strand at 2852.269 kb on + strand at 2852.269 kb on + strand at 2852.269 kb on + strand at 2852.275 kb on + strand at 2852.275 kb on + strand at 2852.477 kb on - strand, within RR42_RS13245 at 2852.477 kb on - strand, within RR42_RS13245 at 2852.477 kb on - strand, within RR42_RS13245 at 2852.605 kb on + strand, within RR42_RS13245 at 2852.606 kb on - strand, within RR42_RS13245 at 2853.014 kb on + strand, within RR42_RS13245 at 2853.014 kb on + strand, within RR42_RS13245 at 2853.014 kb on + strand, within RR42_RS13245 at 2853.015 kb on - strand, within RR42_RS13245 at 2853.015 kb on - strand, within RR42_RS13245 at 2853.015 kb on - strand, within RR42_RS13245 at 2853.025 kb on + strand, within RR42_RS13245 at 2853.130 kb on + strand, within RR42_RS13245 at 2853.163 kb on + strand, within RR42_RS13245 at 2853.166 kb on + strand, within RR42_RS13245 at 2853.166 kb on + strand, within RR42_RS13245 at 2853.167 kb on - strand, within RR42_RS13245 at 2853.167 kb on - strand, within RR42_RS13245 at 2853.308 kb on + strand at 2853.310 kb on + strand at 2853.310 kb on + strand at 2853.311 kb on - strand at 2853.377 kb on + strand at 2853.377 kb on + strand at 2853.378 kb on - strand at 2853.388 kb on + strand at 2853.388 kb on + strand at 2853.388 kb on + strand at 2853.389 kb on - strand at 2853.389 kb on - strand at 2853.455 kb on + strand at 2853.455 kb on + strand at 2853.456 kb on - strand at 2853.456 kb on - strand at 2853.456 kb on - strand at 2853.456 kb on - strand at 2853.497 kb on + strand at 2853.498 kb on - strand at 2853.523 kb on - strand at 2853.523 kb on - strand at 2853.612 kb on + strand at 2853.757 kb on - strand, within RR42_RS13250 at 2853.757 kb on - strand, within RR42_RS13250 at 2853.757 kb on - strand, within RR42_RS13250 at 2853.968 kb on + strand, within RR42_RS13250 at 2853.970 kb on - strand, within RR42_RS13250 at 2853.970 kb on - strand, within RR42_RS13250 at 2853.970 kb on - strand, within RR42_RS13250 at 2853.970 kb on - strand, within RR42_RS13250 at 2854.056 kb on + strand, within RR42_RS13250 at 2854.087 kb on - strand, within RR42_RS13250 at 2854.087 kb on - strand, within RR42_RS13250 at 2854.258 kb on - strand, within RR42_RS13250
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,851,495 + RR42_RS13240 0.29 +0.5 2,851,496 - RR42_RS13240 0.29 -1.1 2,851,609 + RR42_RS13240 0.43 -0.9 2,851,610 - RR42_RS13240 0.43 -0.3 2,851,610 - RR42_RS13240 0.43 +0.5 2,851,820 + RR42_RS13240 0.69 -3.0 2,851,821 - RR42_RS13240 0.69 -1.3 2,851,867 + RR42_RS13240 0.75 -1.2 2,851,913 + RR42_RS13240 0.80 -0.4 2,851,998 + -1.0 2,852,000 + -1.4 2,852,001 - -1.1 2,852,011 - -1.0 2,852,145 + -1.1 2,852,148 + -1.7 2,852,149 - -0.6 2,852,269 + -0.4 2,852,269 + -0.4 2,852,269 + +0.3 2,852,269 + -2.0 2,852,269 + -0.9 2,852,269 + +0.1 2,852,275 + -1.4 2,852,275 + +0.1 2,852,477 - RR42_RS13245 0.20 -1.1 2,852,477 - RR42_RS13245 0.20 -0.8 2,852,477 - RR42_RS13245 0.20 -2.2 2,852,605 + RR42_RS13245 0.32 -1.2 2,852,606 - RR42_RS13245 0.32 -0.4 2,853,014 + RR42_RS13245 0.71 -0.7 2,853,014 + RR42_RS13245 0.71 +0.1 2,853,014 + RR42_RS13245 0.71 -0.4 2,853,015 - RR42_RS13245 0.71 +1.1 2,853,015 - RR42_RS13245 0.71 -1.9 2,853,015 - RR42_RS13245 0.71 +0.1 2,853,025 + RR42_RS13245 0.72 -0.4 2,853,130 + RR42_RS13245 0.82 -0.3 2,853,163 + RR42_RS13245 0.85 -0.9 2,853,166 + RR42_RS13245 0.86 +1.1 2,853,166 + RR42_RS13245 0.86 -1.1 2,853,167 - RR42_RS13245 0.86 -0.7 2,853,167 - RR42_RS13245 0.86 -0.9 2,853,308 + -0.8 2,853,310 + -0.6 2,853,310 + -2.1 2,853,311 - -0.0 2,853,377 + +0.4 2,853,377 + -0.7 2,853,378 - -0.2 2,853,388 + -0.1 2,853,388 + +0.9 2,853,388 + -0.8 2,853,389 - -0.2 2,853,389 - -1.0 2,853,455 + +0.5 2,853,455 + +1.8 2,853,456 - -0.6 2,853,456 - -0.9 2,853,456 - -1.0 2,853,456 - -0.1 2,853,497 + +0.1 2,853,498 - -0.4 2,853,523 - -0.5 2,853,523 - -0.9 2,853,612 + -1.7 2,853,757 - RR42_RS13250 0.22 -0.4 2,853,757 - RR42_RS13250 0.22 -0.5 2,853,757 - RR42_RS13250 0.22 -1.5 2,853,968 + RR42_RS13250 0.44 -0.3 2,853,970 - RR42_RS13250 0.44 -0.2 2,853,970 - RR42_RS13250 0.44 +0.1 2,853,970 - RR42_RS13250 0.44 -0.4 2,853,970 - RR42_RS13250 0.44 -0.3 2,854,056 + RR42_RS13250 0.53 -0.7 2,854,087 - RR42_RS13250 0.57 +0.3 2,854,087 - RR42_RS13250 0.57 -0.5 2,854,258 - RR42_RS13250 0.75 -0.5
Or see this region's nucleotide sequence