Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12765 and RR42_RS12770 are separated by 433 nucleotides RR42_RS12770 and RR42_RS12775 are separated by 58 nucleotides RR42_RS12775 and RR42_RS12780 are separated by 100 nucleotides RR42_RS12780 and RR42_RS12785 are separated by 179 nucleotides
RR42_RS12765: RR42_RS12765 - protein kinase, at 2,741,615 to 2,743,696
_RS12765
RR42_RS12770: RR42_RS12770 - hypothetical protein, at 2,744,130 to 2,744,387
_RS12770
RR42_RS12775: RR42_RS12775 - hypothetical protein, at 2,744,446 to 2,744,703
_RS12775
RR42_RS12780: RR42_RS12780 - hypothetical protein, at 2,744,804 to 2,745,067
_RS12780
RR42_RS12785: RR42_RS12785 - hypothetical protein, at 2,745,247 to 2,745,465
_RS12785
Position (kb)
2744
2745 Strain fitness (log2 ratio)
-1
0
1 at 2743.240 kb on - strand, within RR42_RS12765 at 2743.240 kb on - strand, within RR42_RS12765 at 2743.240 kb on - strand, within RR42_RS12765 at 2743.287 kb on + strand, within RR42_RS12765 at 2743.287 kb on + strand, within RR42_RS12765 at 2743.348 kb on + strand, within RR42_RS12765 at 2743.348 kb on + strand, within RR42_RS12765 at 2743.527 kb on + strand at 2743.528 kb on - strand at 2743.669 kb on + strand at 2743.669 kb on + strand at 2743.673 kb on + strand at 2743.673 kb on + strand at 2743.673 kb on + strand at 2743.673 kb on + strand at 2743.674 kb on - strand at 2743.674 kb on - strand at 2743.764 kb on - strand at 2743.781 kb on - strand at 2743.781 kb on - strand at 2743.783 kb on + strand at 2743.874 kb on + strand at 2743.874 kb on + strand at 2743.875 kb on - strand at 2744.698 kb on + strand at 2745.157 kb on + strand at 2745.158 kb on - strand at 2745.158 kb on - strand at 2745.158 kb on - strand at 2745.158 kb on - strand at 2745.158 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,743,240 - RR42_RS12765 0.78 -0.7 2,743,240 - RR42_RS12765 0.78 -0.8 2,743,240 - RR42_RS12765 0.78 -1.2 2,743,287 + RR42_RS12765 0.80 +0.4 2,743,287 + RR42_RS12765 0.80 -0.8 2,743,348 + RR42_RS12765 0.83 -0.5 2,743,348 + RR42_RS12765 0.83 +0.0 2,743,527 + -0.8 2,743,528 - -1.4 2,743,669 + +0.2 2,743,669 + -1.6 2,743,673 + -1.5 2,743,673 + -0.0 2,743,673 + -0.5 2,743,673 + -0.7 2,743,674 - -0.2 2,743,674 - -1.6 2,743,764 - -0.4 2,743,781 - -1.6 2,743,781 - -1.5 2,743,783 + -0.5 2,743,874 + -0.3 2,743,874 + -0.1 2,743,875 - -1.0 2,744,698 + -0.8 2,745,157 + +1.5 2,745,158 - +0.8 2,745,158 - -1.6 2,745,158 - -0.6 2,745,158 - +1.1 2,745,158 - +0.8
Or see this region's nucleotide sequence