Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12495 and RR42_RS12500 are separated by 112 nucleotides RR42_RS12500 and RR42_RS12505 overlap by 4 nucleotides RR42_RS12505 and RR42_RS12510 are separated by 232 nucleotides
RR42_RS12495: RR42_RS12495 - ABC transporter permease, at 2,687,327 to 2,688,511
_RS12495
RR42_RS12500: RR42_RS12500 - enoyl-CoA hydratase, at 2,688,624 to 2,689,403
_RS12500
RR42_RS12505: RR42_RS12505 - TetR family transcriptional regulator, at 2,689,400 to 2,690,005
_RS12505
RR42_RS12510: RR42_RS12510 - hydrolase, at 2,690,238 to 2,691,077
_RS12510
Position (kb)
2688
2689
2690 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2687.634 kb on + strand, within RR42_RS12495 at 2687.634 kb on + strand, within RR42_RS12495 at 2687.685 kb on + strand, within RR42_RS12495 at 2687.686 kb on - strand, within RR42_RS12495 at 2687.691 kb on + strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.724 kb on + strand, within RR42_RS12495 at 2687.775 kb on + strand, within RR42_RS12495 at 2687.922 kb on + strand, within RR42_RS12495 at 2687.922 kb on + strand, within RR42_RS12495 at 2687.922 kb on + strand, within RR42_RS12495 at 2687.923 kb on - strand, within RR42_RS12495 at 2687.994 kb on + strand, within RR42_RS12495 at 2687.994 kb on + strand, within RR42_RS12495 at 2688.093 kb on + strand, within RR42_RS12495 at 2688.093 kb on + strand, within RR42_RS12495 at 2688.094 kb on - strand, within RR42_RS12495 at 2688.094 kb on - strand, within RR42_RS12495 at 2688.241 kb on - strand, within RR42_RS12495 at 2688.345 kb on + strand, within RR42_RS12495 at 2688.414 kb on + strand at 2688.415 kb on - strand at 2688.415 kb on - strand at 2688.626 kb on - strand at 2688.626 kb on - strand at 2688.626 kb on - strand at 2688.991 kb on + strand, within RR42_RS12500 at 2688.991 kb on + strand, within RR42_RS12500 at 2688.991 kb on + strand, within RR42_RS12500 at 2688.992 kb on - strand, within RR42_RS12500 at 2688.992 kb on - strand, within RR42_RS12500 at 2688.994 kb on + strand, within RR42_RS12500 at 2688.994 kb on + strand, within RR42_RS12500 at 2688.995 kb on - strand, within RR42_RS12500 at 2689.441 kb on + strand at 2689.486 kb on - strand, within RR42_RS12505 at 2689.591 kb on + strand, within RR42_RS12505 at 2689.594 kb on - strand, within RR42_RS12505 at 2689.617 kb on + strand, within RR42_RS12505 at 2689.618 kb on - strand, within RR42_RS12505 at 2689.626 kb on + strand, within RR42_RS12505 at 2689.627 kb on - strand, within RR42_RS12505 at 2689.627 kb on - strand, within RR42_RS12505 at 2689.630 kb on + strand, within RR42_RS12505 at 2689.630 kb on + strand, within RR42_RS12505 at 2689.630 kb on + strand, within RR42_RS12505 at 2689.833 kb on + strand, within RR42_RS12505 at 2689.834 kb on - strand, within RR42_RS12505 at 2689.839 kb on + strand, within RR42_RS12505 at 2689.839 kb on + strand, within RR42_RS12505 at 2689.839 kb on + strand, within RR42_RS12505 at 2689.839 kb on + strand, within RR42_RS12505 at 2689.840 kb on - strand, within RR42_RS12505 at 2689.840 kb on - strand, within RR42_RS12505 at 2689.896 kb on + strand, within RR42_RS12505 at 2689.896 kb on + strand, within RR42_RS12505 at 2689.896 kb on + strand, within RR42_RS12505 at 2689.897 kb on - strand, within RR42_RS12505 at 2689.899 kb on + strand, within RR42_RS12505 at 2689.899 kb on + strand, within RR42_RS12505 at 2690.142 kb on - strand at 2690.149 kb on + strand at 2690.149 kb on + strand at 2690.149 kb on + strand at 2690.149 kb on + strand at 2690.150 kb on - strand at 2690.150 kb on - strand at 2690.150 kb on - strand at 2690.150 kb on - strand at 2690.150 kb on - strand at 2690.150 kb on - strand at 2690.150 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,687,634 + RR42_RS12495 0.26 -0.2 2,687,634 + RR42_RS12495 0.26 +1.3 2,687,685 + RR42_RS12495 0.30 +0.7 2,687,686 - RR42_RS12495 0.30 -1.1 2,687,691 + RR42_RS12495 0.31 -1.0 2,687,692 - RR42_RS12495 0.31 -0.2 2,687,692 - RR42_RS12495 0.31 -0.2 2,687,692 - RR42_RS12495 0.31 +0.1 2,687,692 - RR42_RS12495 0.31 -0.7 2,687,724 + RR42_RS12495 0.34 +1.1 2,687,775 + RR42_RS12495 0.38 +0.2 2,687,922 + RR42_RS12495 0.50 -1.3 2,687,922 + RR42_RS12495 0.50 -0.0 2,687,922 + RR42_RS12495 0.50 +0.1 2,687,923 - RR42_RS12495 0.50 +1.8 2,687,994 + RR42_RS12495 0.56 -0.4 2,687,994 + RR42_RS12495 0.56 -0.5 2,688,093 + RR42_RS12495 0.65 -0.8 2,688,093 + RR42_RS12495 0.65 +1.5 2,688,094 - RR42_RS12495 0.65 -0.5 2,688,094 - RR42_RS12495 0.65 -0.5 2,688,241 - RR42_RS12495 0.77 -0.4 2,688,345 + RR42_RS12495 0.86 +0.4 2,688,414 + +0.2 2,688,415 - +0.2 2,688,415 - +0.8 2,688,626 - -0.0 2,688,626 - +0.5 2,688,626 - -1.5 2,688,991 + RR42_RS12500 0.47 -0.3 2,688,991 + RR42_RS12500 0.47 +3.1 2,688,991 + RR42_RS12500 0.47 +0.4 2,688,992 - RR42_RS12500 0.47 +1.0 2,688,992 - RR42_RS12500 0.47 +0.1 2,688,994 + RR42_RS12500 0.47 +0.5 2,688,994 + RR42_RS12500 0.47 -0.2 2,688,995 - RR42_RS12500 0.48 -0.5 2,689,441 + +0.6 2,689,486 - RR42_RS12505 0.14 +1.2 2,689,591 + RR42_RS12505 0.32 +0.2 2,689,594 - RR42_RS12505 0.32 +0.4 2,689,617 + RR42_RS12505 0.36 -0.3 2,689,618 - RR42_RS12505 0.36 -0.7 2,689,626 + RR42_RS12505 0.37 +2.1 2,689,627 - RR42_RS12505 0.37 +0.8 2,689,627 - RR42_RS12505 0.37 +2.2 2,689,630 + RR42_RS12505 0.38 +0.7 2,689,630 + RR42_RS12505 0.38 +2.4 2,689,630 + RR42_RS12505 0.38 -0.7 2,689,833 + RR42_RS12505 0.71 +0.2 2,689,834 - RR42_RS12505 0.72 -0.9 2,689,839 + RR42_RS12505 0.72 -0.8 2,689,839 + RR42_RS12505 0.72 +0.1 2,689,839 + RR42_RS12505 0.72 -0.2 2,689,839 + RR42_RS12505 0.72 -1.3 2,689,840 - RR42_RS12505 0.73 +0.1 2,689,840 - RR42_RS12505 0.73 -0.8 2,689,896 + RR42_RS12505 0.82 -1.5 2,689,896 + RR42_RS12505 0.82 -0.1 2,689,896 + RR42_RS12505 0.82 +0.5 2,689,897 - RR42_RS12505 0.82 +0.9 2,689,899 + RR42_RS12505 0.82 -0.2 2,689,899 + RR42_RS12505 0.82 -0.7 2,690,142 - +0.7 2,690,149 + +0.1 2,690,149 + +0.8 2,690,149 + +1.1 2,690,149 + +0.1 2,690,150 - -0.0 2,690,150 - -2.1 2,690,150 - +0.6 2,690,150 - -1.3 2,690,150 - -0.2 2,690,150 - -0.8 2,690,150 - +0.9
Or see this region's nucleotide sequence