Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12090 and RR42_RS12095 are separated by 12 nucleotides RR42_RS12095 and RR42_RS12100 overlap by 4 nucleotides
RR42_RS12090: RR42_RS12090 - monooxygenase, at 2,590,063 to 2,591,250
_RS12090
RR42_RS12095: RR42_RS12095 - monooxygenase, at 2,591,263 to 2,592,510
_RS12095
RR42_RS12100: RR42_RS12100 - monooxygenase, at 2,592,507 to 2,593,859
_RS12100
Position (kb)
2591
2592
2593 Strain fitness (log2 ratio)
-2
-1
0
1 at 2590.823 kb on - strand, within RR42_RS12090 at 2590.975 kb on + strand, within RR42_RS12090 at 2590.975 kb on + strand, within RR42_RS12090 at 2590.975 kb on + strand, within RR42_RS12090 at 2590.975 kb on + strand, within RR42_RS12090 at 2590.976 kb on - strand, within RR42_RS12090 at 2590.976 kb on - strand, within RR42_RS12090 at 2590.976 kb on - strand, within RR42_RS12090 at 2591.215 kb on - strand at 2591.242 kb on + strand at 2591.242 kb on + strand at 2591.243 kb on - strand at 2591.243 kb on - strand at 2591.575 kb on + strand, within RR42_RS12095 at 2591.576 kb on - strand, within RR42_RS12095 at 2591.911 kb on + strand, within RR42_RS12095 at 2591.912 kb on - strand, within RR42_RS12095 at 2592.082 kb on + strand, within RR42_RS12095 at 2592.420 kb on + strand at 2592.421 kb on - strand at 2592.421 kb on - strand at 2592.421 kb on - strand at 2592.443 kb on - strand at 2592.503 kb on - strand at 2592.504 kb on - strand at 2592.637 kb on - strand at 2592.789 kb on - strand, within RR42_RS12100 at 2592.819 kb on + strand, within RR42_RS12100 at 2592.831 kb on + strand, within RR42_RS12100 at 2592.831 kb on + strand, within RR42_RS12100 at 2592.831 kb on + strand, within RR42_RS12100 at 2592.832 kb on - strand, within RR42_RS12100 at 2592.859 kb on + strand, within RR42_RS12100
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,590,823 - RR42_RS12090 0.64 +0.4 2,590,975 + RR42_RS12090 0.77 -0.7 2,590,975 + RR42_RS12090 0.77 +0.3 2,590,975 + RR42_RS12090 0.77 +0.3 2,590,975 + RR42_RS12090 0.77 +0.2 2,590,976 - RR42_RS12090 0.77 -0.7 2,590,976 - RR42_RS12090 0.77 -0.7 2,590,976 - RR42_RS12090 0.77 -0.7 2,591,215 - +0.4 2,591,242 + -1.9 2,591,242 + -0.0 2,591,243 - +0.1 2,591,243 - -2.2 2,591,575 + RR42_RS12095 0.25 +0.3 2,591,576 - RR42_RS12095 0.25 -1.2 2,591,911 + RR42_RS12095 0.52 -0.7 2,591,912 - RR42_RS12095 0.52 -1.3 2,592,082 + RR42_RS12095 0.66 -0.5 2,592,420 + -0.6 2,592,421 - +0.2 2,592,421 - -0.2 2,592,421 - -0.7 2,592,443 - +0.0 2,592,503 - -0.7 2,592,504 - -0.2 2,592,637 - -0.8 2,592,789 - RR42_RS12100 0.21 -0.9 2,592,819 + RR42_RS12100 0.23 +0.1 2,592,831 + RR42_RS12100 0.24 +0.9 2,592,831 + RR42_RS12100 0.24 -0.3 2,592,831 + RR42_RS12100 0.24 +0.4 2,592,832 - RR42_RS12100 0.24 +0.3 2,592,859 + RR42_RS12100 0.26 +0.3
Or see this region's nucleotide sequence