Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10985 and RR42_RS10990 are separated by 79 nucleotides RR42_RS10990 and RR42_RS10995 are separated by 111 nucleotides RR42_RS10995 and RR42_RS11000 are separated by 116 nucleotides
RR42_RS10985: RR42_RS10985 - NAD-dependent epimerase, at 2,346,324 to 2,347,313
_RS10985
RR42_RS10990: RR42_RS10990 - MFS transporter, at 2,347,393 to 2,347,830
_RS10990
RR42_RS10995: RR42_RS10995 - hypothetical protein, at 2,347,942 to 2,348,928
_RS10995
RR42_RS11000: RR42_RS11000 - alpha/beta hydrolase, at 2,349,045 to 2,350,010
_RS11000
Position (kb)
2347
2348
2349 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2347.187 kb on + strand, within RR42_RS10985 at 2347.188 kb on - strand, within RR42_RS10985 at 2347.281 kb on + strand at 2347.282 kb on - strand at 2347.283 kb on + strand at 2347.283 kb on + strand at 2347.283 kb on + strand at 2347.283 kb on + strand at 2347.284 kb on - strand at 2347.300 kb on + strand at 2347.418 kb on + strand at 2347.418 kb on + strand at 2347.418 kb on + strand at 2347.419 kb on - strand at 2347.490 kb on + strand, within RR42_RS10990 at 2347.491 kb on - strand, within RR42_RS10990 at 2347.491 kb on - strand, within RR42_RS10990 at 2347.491 kb on - strand, within RR42_RS10990 at 2347.529 kb on + strand, within RR42_RS10990 at 2347.529 kb on + strand, within RR42_RS10990 at 2347.530 kb on - strand, within RR42_RS10990 at 2347.709 kb on + strand, within RR42_RS10990 at 2347.725 kb on + strand, within RR42_RS10990 at 2347.726 kb on - strand, within RR42_RS10990 at 2347.923 kb on + strand at 2347.924 kb on - strand at 2347.932 kb on + strand at 2347.944 kb on - strand at 2347.944 kb on - strand at 2347.944 kb on - strand at 2348.018 kb on + strand at 2348.018 kb on + strand at 2348.019 kb on - strand at 2348.035 kb on - strand at 2348.036 kb on + strand at 2348.037 kb on - strand at 2348.037 kb on - strand at 2348.075 kb on + strand, within RR42_RS10995 at 2348.075 kb on + strand, within RR42_RS10995 at 2348.279 kb on + strand, within RR42_RS10995 at 2348.279 kb on + strand, within RR42_RS10995 at 2348.280 kb on - strand, within RR42_RS10995 at 2348.360 kb on + strand, within RR42_RS10995 at 2348.447 kb on + strand, within RR42_RS10995 at 2348.447 kb on + strand, within RR42_RS10995 at 2348.486 kb on + strand, within RR42_RS10995 at 2348.486 kb on + strand, within RR42_RS10995 at 2348.486 kb on + strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.487 kb on - strand, within RR42_RS10995 at 2348.561 kb on + strand, within RR42_RS10995 at 2348.562 kb on - strand, within RR42_RS10995 at 2348.604 kb on - strand, within RR42_RS10995 at 2348.646 kb on - strand, within RR42_RS10995 at 2348.955 kb on + strand at 2348.955 kb on + strand at 2348.955 kb on + strand at 2348.955 kb on + strand at 2348.955 kb on + strand at 2348.955 kb on + strand at 2348.956 kb on - strand at 2348.956 kb on - strand at 2348.961 kb on + strand at 2348.961 kb on + strand at 2348.961 kb on + strand at 2348.961 kb on + strand at 2348.961 kb on + strand at 2348.961 kb on + strand at 2348.961 kb on + strand at 2348.962 kb on - strand at 2348.962 kb on - strand at 2348.969 kb on + strand at 2348.975 kb on + strand at 2348.976 kb on - strand at 2349.151 kb on + strand, within RR42_RS11000 at 2349.152 kb on - strand, within RR42_RS11000 at 2349.152 kb on - strand, within RR42_RS11000 at 2349.230 kb on - strand, within RR42_RS11000 at 2349.341 kb on - strand, within RR42_RS11000 at 2349.341 kb on - strand, within RR42_RS11000 at 2349.382 kb on + strand, within RR42_RS11000 at 2349.439 kb on + strand, within RR42_RS11000 at 2349.439 kb on + strand, within RR42_RS11000 at 2349.439 kb on + strand, within RR42_RS11000 at 2349.440 kb on - strand, within RR42_RS11000 at 2349.440 kb on - strand, within RR42_RS11000 at 2349.440 kb on - strand, within RR42_RS11000 at 2349.440 kb on - strand, within RR42_RS11000 at 2349.646 kb on + strand, within RR42_RS11000 at 2349.749 kb on - strand, within RR42_RS11000 at 2349.796 kb on + strand, within RR42_RS11000 at 2349.797 kb on - strand, within RR42_RS11000
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,347,187 + RR42_RS10985 0.87 -0.6 2,347,188 - RR42_RS10985 0.87 -0.4 2,347,281 + -0.7 2,347,282 - -0.1 2,347,283 + +2.3 2,347,283 + -0.6 2,347,283 + -0.5 2,347,283 + -0.2 2,347,284 - +0.1 2,347,300 + +0.0 2,347,418 + -0.3 2,347,418 + +1.2 2,347,418 + -0.3 2,347,419 - +0.8 2,347,490 + RR42_RS10990 0.22 -0.3 2,347,491 - RR42_RS10990 0.22 +0.8 2,347,491 - RR42_RS10990 0.22 -0.3 2,347,491 - RR42_RS10990 0.22 +0.1 2,347,529 + RR42_RS10990 0.31 -0.4 2,347,529 + RR42_RS10990 0.31 +0.2 2,347,530 - RR42_RS10990 0.31 -0.1 2,347,709 + RR42_RS10990 0.72 -0.5 2,347,725 + RR42_RS10990 0.76 +0.7 2,347,726 - RR42_RS10990 0.76 -0.1 2,347,923 + +0.1 2,347,924 - +0.1 2,347,932 + -0.8 2,347,944 - +0.1 2,347,944 - -0.4 2,347,944 - -1.0 2,348,018 + -0.3 2,348,018 + +0.3 2,348,019 - +0.3 2,348,035 - -0.3 2,348,036 + +0.0 2,348,037 - -0.0 2,348,037 - +0.2 2,348,075 + RR42_RS10995 0.13 +1.4 2,348,075 + RR42_RS10995 0.13 -1.2 2,348,279 + RR42_RS10995 0.34 +0.0 2,348,279 + RR42_RS10995 0.34 -0.1 2,348,280 - RR42_RS10995 0.34 +1.5 2,348,360 + RR42_RS10995 0.42 +0.3 2,348,447 + RR42_RS10995 0.51 -0.5 2,348,447 + RR42_RS10995 0.51 -0.8 2,348,486 + RR42_RS10995 0.55 +1.4 2,348,486 + RR42_RS10995 0.55 -0.0 2,348,486 + RR42_RS10995 0.55 -0.5 2,348,487 - RR42_RS10995 0.55 +0.2 2,348,487 - RR42_RS10995 0.55 -0.6 2,348,487 - RR42_RS10995 0.55 -0.9 2,348,487 - RR42_RS10995 0.55 +0.5 2,348,487 - RR42_RS10995 0.55 -1.4 2,348,487 - RR42_RS10995 0.55 -1.0 2,348,487 - RR42_RS10995 0.55 -1.1 2,348,487 - RR42_RS10995 0.55 -0.2 2,348,561 + RR42_RS10995 0.63 -0.6 2,348,562 - RR42_RS10995 0.63 +0.2 2,348,604 - RR42_RS10995 0.67 +0.5 2,348,646 - RR42_RS10995 0.71 -0.8 2,348,955 + -0.3 2,348,955 + -0.1 2,348,955 + -0.6 2,348,955 + -0.1 2,348,955 + -0.2 2,348,955 + -1.0 2,348,956 - -0.0 2,348,956 - +0.2 2,348,961 + -1.3 2,348,961 + -0.2 2,348,961 + -1.0 2,348,961 + -0.1 2,348,961 + -0.1 2,348,961 + -0.2 2,348,961 + -1.0 2,348,962 - -1.3 2,348,962 - -1.9 2,348,969 + +1.4 2,348,975 + +1.8 2,348,976 - -0.2 2,349,151 + RR42_RS11000 0.11 -1.2 2,349,152 - RR42_RS11000 0.11 -0.8 2,349,152 - RR42_RS11000 0.11 -1.1 2,349,230 - RR42_RS11000 0.19 -0.2 2,349,341 - RR42_RS11000 0.31 -0.2 2,349,341 - RR42_RS11000 0.31 -0.2 2,349,382 + RR42_RS11000 0.35 -0.2 2,349,439 + RR42_RS11000 0.41 +0.3 2,349,439 + RR42_RS11000 0.41 +1.6 2,349,439 + RR42_RS11000 0.41 +0.1 2,349,440 - RR42_RS11000 0.41 +0.4 2,349,440 - RR42_RS11000 0.41 +1.5 2,349,440 - RR42_RS11000 0.41 -0.9 2,349,440 - RR42_RS11000 0.41 -0.2 2,349,646 + RR42_RS11000 0.62 -0.8 2,349,749 - RR42_RS11000 0.73 +0.2 2,349,796 + RR42_RS11000 0.78 -0.5 2,349,797 - RR42_RS11000 0.78 -0.2
Or see this region's nucleotide sequence