Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10640 and RR42_RS10645 are separated by 154 nucleotides RR42_RS10645 and RR42_RS10650 are separated by 177 nucleotides RR42_RS10650 and RR42_RS10655 overlap by 4 nucleotides
RR42_RS10640: RR42_RS10640 - alanine dehydrogenase, at 2,273,201 to 2,274,316
_RS10640
RR42_RS10645: RR42_RS10645 - hypothetical protein, at 2,274,471 to 2,274,818
_RS10645
RR42_RS10650: RR42_RS10650 - acetyl-CoA synthetase, at 2,274,996 to 2,276,762
_RS10650
RR42_RS10655: RR42_RS10655 - pyruvate dehydrogenase, at 2,276,759 to 2,277,781
_RS10655
Position (kb)
2274
2275
2276
2277 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2274.037 kb on - strand, within RR42_RS10640 at 2274.037 kb on - strand, within RR42_RS10640 at 2274.043 kb on - strand, within RR42_RS10640 at 2274.125 kb on + strand, within RR42_RS10640 at 2274.146 kb on + strand, within RR42_RS10640 at 2274.146 kb on + strand, within RR42_RS10640 at 2274.147 kb on - strand, within RR42_RS10640 at 2274.169 kb on - strand, within RR42_RS10640 at 2274.251 kb on - strand at 2274.472 kb on + strand at 2274.472 kb on + strand at 2274.473 kb on - strand at 2274.549 kb on + strand, within RR42_RS10645 at 2274.549 kb on + strand, within RR42_RS10645 at 2274.654 kb on + strand, within RR42_RS10645 at 2274.654 kb on + strand, within RR42_RS10645 at 2274.655 kb on - strand, within RR42_RS10645 at 2274.655 kb on - strand, within RR42_RS10645 at 2274.656 kb on + strand, within RR42_RS10645 at 2274.657 kb on - strand, within RR42_RS10645 at 2274.657 kb on - strand, within RR42_RS10645 at 2274.751 kb on - strand, within RR42_RS10645 at 2274.751 kb on - strand, within RR42_RS10645 at 2274.767 kb on - strand, within RR42_RS10645 at 2274.767 kb on - strand, within RR42_RS10645 at 2274.894 kb on - strand at 2274.894 kb on - strand at 2274.895 kb on + strand at 2274.895 kb on + strand at 2274.895 kb on + strand at 2274.895 kb on + strand at 2274.895 kb on + strand at 2274.895 kb on + strand at 2274.896 kb on - strand at 2274.959 kb on - strand at 2274.966 kb on + strand at 2274.967 kb on - strand at 2274.967 kb on - strand at 2275.175 kb on + strand, within RR42_RS10650 at 2275.239 kb on + strand, within RR42_RS10650 at 2275.348 kb on - strand, within RR42_RS10650 at 2275.654 kb on + strand, within RR42_RS10650 at 2275.655 kb on - strand, within RR42_RS10650 at 2275.773 kb on + strand, within RR42_RS10650 at 2275.773 kb on + strand, within RR42_RS10650 at 2275.773 kb on + strand, within RR42_RS10650 at 2275.782 kb on - strand, within RR42_RS10650 at 2275.815 kb on + strand, within RR42_RS10650 at 2275.890 kb on + strand, within RR42_RS10650 at 2275.926 kb on + strand, within RR42_RS10650 at 2275.926 kb on + strand, within RR42_RS10650 at 2275.986 kb on + strand, within RR42_RS10650 at 2276.228 kb on - strand, within RR42_RS10650 at 2276.228 kb on - strand, within RR42_RS10650 at 2276.230 kb on + strand, within RR42_RS10650 at 2276.231 kb on - strand, within RR42_RS10650 at 2276.231 kb on - strand, within RR42_RS10650 at 2276.382 kb on + strand, within RR42_RS10650 at 2276.383 kb on - strand, within RR42_RS10650 at 2276.386 kb on - strand, within RR42_RS10650 at 2276.386 kb on - strand, within RR42_RS10650 at 2276.386 kb on - strand, within RR42_RS10650 at 2276.518 kb on + strand, within RR42_RS10650 at 2276.519 kb on - strand, within RR42_RS10650 at 2276.736 kb on - strand at 2276.816 kb on - strand at 2276.889 kb on - strand, within RR42_RS10655 at 2276.904 kb on + strand, within RR42_RS10655 at 2276.905 kb on - strand, within RR42_RS10655 at 2276.924 kb on + strand, within RR42_RS10655 at 2276.924 kb on + strand, within RR42_RS10655 at 2276.925 kb on - strand, within RR42_RS10655 at 2276.996 kb on + strand, within RR42_RS10655 at 2276.997 kb on - strand, within RR42_RS10655 at 2277.059 kb on + strand, within RR42_RS10655 at 2277.395 kb on + strand, within RR42_RS10655 at 2277.396 kb on - strand, within RR42_RS10655 at 2277.401 kb on - strand, within RR42_RS10655 at 2277.401 kb on - strand, within RR42_RS10655 at 2277.401 kb on - strand, within RR42_RS10655 at 2277.515 kb on + strand, within RR42_RS10655 at 2277.516 kb on - strand, within RR42_RS10655 at 2277.540 kb on - strand, within RR42_RS10655 at 2277.554 kb on + strand, within RR42_RS10655 at 2277.604 kb on + strand, within RR42_RS10655 at 2277.605 kb on - strand, within RR42_RS10655 at 2277.605 kb on - strand, within RR42_RS10655 at 2277.755 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,274,037 - RR42_RS10640 0.75 +0.5 2,274,037 - RR42_RS10640 0.75 +0.1 2,274,043 - RR42_RS10640 0.75 -0.4 2,274,125 + RR42_RS10640 0.83 -0.3 2,274,146 + RR42_RS10640 0.85 -0.4 2,274,146 + RR42_RS10640 0.85 -0.1 2,274,147 - RR42_RS10640 0.85 +0.5 2,274,169 - RR42_RS10640 0.87 -0.2 2,274,251 - -0.7 2,274,472 + -0.3 2,274,472 + -1.0 2,274,473 - +0.0 2,274,549 + RR42_RS10645 0.22 -0.4 2,274,549 + RR42_RS10645 0.22 +0.0 2,274,654 + RR42_RS10645 0.53 +0.3 2,274,654 + RR42_RS10645 0.53 +1.2 2,274,655 - RR42_RS10645 0.53 -0.5 2,274,655 - RR42_RS10645 0.53 -0.3 2,274,656 + RR42_RS10645 0.53 +0.6 2,274,657 - RR42_RS10645 0.53 +1.7 2,274,657 - RR42_RS10645 0.53 +1.2 2,274,751 - RR42_RS10645 0.80 -0.1 2,274,751 - RR42_RS10645 0.80 -0.3 2,274,767 - RR42_RS10645 0.85 +0.2 2,274,767 - RR42_RS10645 0.85 -1.0 2,274,894 - -0.2 2,274,894 - -0.2 2,274,895 + +0.0 2,274,895 + -2.4 2,274,895 + +0.7 2,274,895 + +0.1 2,274,895 + -1.5 2,274,895 + +0.2 2,274,896 - +0.1 2,274,959 - -0.4 2,274,966 + -0.6 2,274,967 - +0.1 2,274,967 - -0.5 2,275,175 + RR42_RS10650 0.10 +0.6 2,275,239 + RR42_RS10650 0.14 -0.1 2,275,348 - RR42_RS10650 0.20 +0.3 2,275,654 + RR42_RS10650 0.37 +0.2 2,275,655 - RR42_RS10650 0.37 +0.4 2,275,773 + RR42_RS10650 0.44 +0.4 2,275,773 + RR42_RS10650 0.44 -0.3 2,275,773 + RR42_RS10650 0.44 -0.3 2,275,782 - RR42_RS10650 0.44 +0.4 2,275,815 + RR42_RS10650 0.46 -2.0 2,275,890 + RR42_RS10650 0.51 +0.2 2,275,926 + RR42_RS10650 0.53 -0.7 2,275,926 + RR42_RS10650 0.53 -0.8 2,275,986 + RR42_RS10650 0.56 -0.2 2,276,228 - RR42_RS10650 0.70 -1.5 2,276,228 - RR42_RS10650 0.70 -0.4 2,276,230 + RR42_RS10650 0.70 -0.5 2,276,231 - RR42_RS10650 0.70 -0.2 2,276,231 - RR42_RS10650 0.70 -0.4 2,276,382 + RR42_RS10650 0.78 -0.6 2,276,383 - RR42_RS10650 0.78 -0.6 2,276,386 - RR42_RS10650 0.79 +0.2 2,276,386 - RR42_RS10650 0.79 -0.6 2,276,386 - RR42_RS10650 0.79 +0.0 2,276,518 + RR42_RS10650 0.86 -0.2 2,276,519 - RR42_RS10650 0.86 -0.4 2,276,736 - +0.0 2,276,816 - -3.0 2,276,889 - RR42_RS10655 0.13 +0.8 2,276,904 + RR42_RS10655 0.14 +0.1 2,276,905 - RR42_RS10655 0.14 -1.8 2,276,924 + RR42_RS10655 0.16 -0.2 2,276,924 + RR42_RS10655 0.16 +0.2 2,276,925 - RR42_RS10655 0.16 -0.3 2,276,996 + RR42_RS10655 0.23 -0.9 2,276,997 - RR42_RS10655 0.23 +0.5 2,277,059 + RR42_RS10655 0.29 -0.5 2,277,395 + RR42_RS10655 0.62 -1.6 2,277,396 - RR42_RS10655 0.62 -0.8 2,277,401 - RR42_RS10655 0.63 +0.4 2,277,401 - RR42_RS10655 0.63 -0.6 2,277,401 - RR42_RS10655 0.63 -0.4 2,277,515 + RR42_RS10655 0.74 +0.3 2,277,516 - RR42_RS10655 0.74 +1.4 2,277,540 - RR42_RS10655 0.76 -0.6 2,277,554 + RR42_RS10655 0.78 -0.4 2,277,604 + RR42_RS10655 0.83 -0.6 2,277,605 - RR42_RS10655 0.83 -0.3 2,277,605 - RR42_RS10655 0.83 +0.9 2,277,755 + +0.0
Or see this region's nucleotide sequence