Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10340 and RR42_RS10345 are separated by 168 nucleotides RR42_RS10345 and RR42_RS10350 are separated by 180 nucleotides
RR42_RS10340: RR42_RS10340 - GntR family transcriptional regulator, at 2,212,791 to 2,213,579
_RS10340
RR42_RS10345: RR42_RS10345 - amino acid dehydrogenase, at 2,213,748 to 2,214,818
_RS10345
RR42_RS10350: RR42_RS10350 - indolepyruvate ferredoxin oxidoreductase, at 2,214,999 to 2,218,484
_RS10350
Position (kb)
2213
2214
2215 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2212.872 kb on + strand, within RR42_RS10340 at 2213.045 kb on - strand, within RR42_RS10340 at 2213.045 kb on - strand, within RR42_RS10340 at 2213.508 kb on + strand at 2213.509 kb on - strand at 2213.592 kb on - strand at 2213.647 kb on + strand at 2213.648 kb on - strand at 2213.648 kb on - strand at 2213.648 kb on - strand at 2213.648 kb on - strand at 2213.691 kb on + strand at 2213.793 kb on - strand at 2213.902 kb on + strand, within RR42_RS10345 at 2213.902 kb on + strand, within RR42_RS10345 at 2213.903 kb on - strand, within RR42_RS10345 at 2213.945 kb on - strand, within RR42_RS10345 at 2213.956 kb on + strand, within RR42_RS10345 at 2214.011 kb on + strand, within RR42_RS10345 at 2214.123 kb on + strand, within RR42_RS10345 at 2214.282 kb on + strand, within RR42_RS10345 at 2214.283 kb on - strand, within RR42_RS10345 at 2214.466 kb on + strand, within RR42_RS10345 at 2214.467 kb on - strand, within RR42_RS10345 at 2214.467 kb on - strand, within RR42_RS10345 at 2214.619 kb on - strand, within RR42_RS10345 at 2214.797 kb on + strand at 2214.797 kb on + strand at 2214.797 kb on + strand at 2214.798 kb on - strand at 2214.801 kb on + strand at 2214.848 kb on - strand at 2214.942 kb on + strand at 2214.942 kb on + strand at 2214.943 kb on - strand at 2214.943 kb on - strand at 2214.943 kb on - strand at 2214.946 kb on + strand at 2214.946 kb on + strand at 2214.946 kb on + strand at 2214.947 kb on - strand at 2214.950 kb on + strand at 2215.004 kb on - strand at 2215.004 kb on - strand at 2215.125 kb on - strand at 2215.426 kb on + strand, within RR42_RS10350 at 2215.427 kb on - strand, within RR42_RS10350 at 2215.427 kb on - strand, within RR42_RS10350 at 2215.439 kb on - strand, within RR42_RS10350 at 2215.555 kb on + strand, within RR42_RS10350 at 2215.556 kb on - strand, within RR42_RS10350 at 2215.812 kb on - strand, within RR42_RS10350
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,212,872 + RR42_RS10340 0.10 +0.2 2,213,045 - RR42_RS10340 0.32 -0.8 2,213,045 - RR42_RS10340 0.32 +0.2 2,213,508 + +1.4 2,213,509 - -1.0 2,213,592 - -0.1 2,213,647 + +0.2 2,213,648 - -0.4 2,213,648 - -0.7 2,213,648 - -1.1 2,213,648 - -0.4 2,213,691 + +0.0 2,213,793 - -1.2 2,213,902 + RR42_RS10345 0.14 -0.5 2,213,902 + RR42_RS10345 0.14 +0.3 2,213,903 - RR42_RS10345 0.14 -1.1 2,213,945 - RR42_RS10345 0.18 -0.9 2,213,956 + RR42_RS10345 0.19 +0.2 2,214,011 + RR42_RS10345 0.25 -0.0 2,214,123 + RR42_RS10345 0.35 -0.2 2,214,282 + RR42_RS10345 0.50 +0.2 2,214,283 - RR42_RS10345 0.50 -1.2 2,214,466 + RR42_RS10345 0.67 -0.7 2,214,467 - RR42_RS10345 0.67 -0.3 2,214,467 - RR42_RS10345 0.67 -0.1 2,214,619 - RR42_RS10345 0.81 -1.0 2,214,797 + -0.0 2,214,797 + +1.3 2,214,797 + -0.6 2,214,798 - -0.2 2,214,801 + -0.0 2,214,848 - -1.0 2,214,942 + +0.5 2,214,942 + +0.5 2,214,943 - +0.0 2,214,943 - -0.3 2,214,943 - -0.2 2,214,946 + +1.0 2,214,946 + -1.6 2,214,946 + -0.2 2,214,947 - +2.0 2,214,950 + +0.9 2,215,004 - +0.3 2,215,004 - -0.3 2,215,125 - -1.1 2,215,426 + RR42_RS10350 0.12 -0.6 2,215,427 - RR42_RS10350 0.12 +0.6 2,215,427 - RR42_RS10350 0.12 +0.2 2,215,439 - RR42_RS10350 0.13 +0.1 2,215,555 + RR42_RS10350 0.16 -2.6 2,215,556 - RR42_RS10350 0.16 -0.2 2,215,812 - RR42_RS10350 0.23 -0.2
Or see this region's nucleotide sequence