Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10325 and RR42_RS10330 are separated by 21 nucleotides RR42_RS10330 and RR42_RS10335 overlap by 1 nucleotides RR42_RS10335 and RR42_RS10340 are separated by 112 nucleotides
RR42_RS10325: RR42_RS10325 - 5-oxoprolinase, at 2,208,318 to 2,210,390
_RS10325
RR42_RS10330: RR42_RS10330 - hydantoinase, at 2,210,412 to 2,212,199
_RS10330
RR42_RS10335: RR42_RS10335 - hypothetical protein, at 2,212,199 to 2,212,678
_RS10335
RR42_RS10340: RR42_RS10340 - GntR family transcriptional regulator, at 2,212,791 to 2,213,579
_RS10340
Position (kb)
2210
2211
2212
2213 Strain fitness (log2 ratio)
-1
0
1 at 2209.461 kb on + strand, within RR42_RS10325 at 2209.462 kb on - strand, within RR42_RS10325 at 2209.528 kb on + strand, within RR42_RS10325 at 2209.528 kb on + strand, within RR42_RS10325 at 2209.608 kb on - strand, within RR42_RS10325 at 2209.823 kb on + strand, within RR42_RS10325 at 2210.006 kb on + strand, within RR42_RS10325 at 2210.388 kb on + strand at 2210.540 kb on + strand at 2210.838 kb on + strand, within RR42_RS10330 at 2210.838 kb on + strand, within RR42_RS10330 at 2210.838 kb on + strand, within RR42_RS10330 at 2210.838 kb on + strand, within RR42_RS10330 at 2210.838 kb on + strand, within RR42_RS10330 at 2210.839 kb on - strand, within RR42_RS10330 at 2210.839 kb on - strand, within RR42_RS10330 at 2210.839 kb on - strand, within RR42_RS10330 at 2210.839 kb on - strand, within RR42_RS10330 at 2210.862 kb on + strand, within RR42_RS10330 at 2210.863 kb on - strand, within RR42_RS10330 at 2211.350 kb on + strand, within RR42_RS10330 at 2211.680 kb on - strand, within RR42_RS10330 at 2212.001 kb on - strand, within RR42_RS10330 at 2212.008 kb on + strand, within RR42_RS10330 at 2212.067 kb on + strand at 2212.067 kb on + strand at 2212.068 kb on - strand at 2212.068 kb on - strand at 2212.068 kb on - strand at 2212.872 kb on + strand, within RR42_RS10340 at 2213.045 kb on - strand, within RR42_RS10340 at 2213.045 kb on - strand, within RR42_RS10340
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,209,461 + RR42_RS10325 0.55 -0.4 2,209,462 - RR42_RS10325 0.55 -0.9 2,209,528 + RR42_RS10325 0.58 +0.5 2,209,528 + RR42_RS10325 0.58 +0.4 2,209,608 - RR42_RS10325 0.62 +0.8 2,209,823 + RR42_RS10325 0.73 +0.4 2,210,006 + RR42_RS10325 0.81 -0.2 2,210,388 + +0.4 2,210,540 + +0.0 2,210,838 + RR42_RS10330 0.24 -0.4 2,210,838 + RR42_RS10330 0.24 +0.8 2,210,838 + RR42_RS10330 0.24 +1.6 2,210,838 + RR42_RS10330 0.24 +1.2 2,210,838 + RR42_RS10330 0.24 +0.2 2,210,839 - RR42_RS10330 0.24 -0.2 2,210,839 - RR42_RS10330 0.24 -1.2 2,210,839 - RR42_RS10330 0.24 -1.2 2,210,839 - RR42_RS10330 0.24 -0.8 2,210,862 + RR42_RS10330 0.25 -0.0 2,210,863 - RR42_RS10330 0.25 +0.4 2,211,350 + RR42_RS10330 0.52 -1.5 2,211,680 - RR42_RS10330 0.71 -0.2 2,212,001 - RR42_RS10330 0.89 -0.8 2,212,008 + RR42_RS10330 0.89 +0.0 2,212,067 + -0.8 2,212,067 + -0.2 2,212,068 - -0.1 2,212,068 - +0.1 2,212,068 - -0.3 2,212,872 + RR42_RS10340 0.10 +0.2 2,213,045 - RR42_RS10340 0.32 -0.8 2,213,045 - RR42_RS10340 0.32 +0.2
Or see this region's nucleotide sequence