Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09560 and RR42_RS09565 are separated by 10 nucleotides RR42_RS09565 and RR42_RS09570 are separated by 36 nucleotides RR42_RS09570 and RR42_RS09575 are separated by 198 nucleotides RR42_RS09575 and RR42_RS09580 overlap by 4 nucleotides
RR42_RS09560: RR42_RS09560 - hypothetical protein, at 2,025,803 to 2,026,411
_RS09560
RR42_RS09565: RR42_RS09565 - hypothetical protein, at 2,026,422 to 2,026,952
_RS09565
RR42_RS09570: RR42_RS09570 - hypothetical protein, at 2,026,989 to 2,028,572
_RS09570
RR42_RS09575: RR42_RS09575 - hypothetical protein, at 2,028,771 to 2,029,058
_RS09575
RR42_RS09580: RR42_RS09580 - hypothetical protein, at 2,029,055 to 2,029,336
_RS09580
Position (kb)
2026
2027
2028
2029 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2026.038 kb on + strand, within RR42_RS09560 at 2026.039 kb on - strand, within RR42_RS09560 at 2026.039 kb on - strand, within RR42_RS09560 at 2026.131 kb on - strand, within RR42_RS09560 at 2026.306 kb on + strand, within RR42_RS09560 at 2026.307 kb on - strand, within RR42_RS09560 at 2026.393 kb on - strand at 2026.443 kb on + strand at 2026.444 kb on - strand at 2026.447 kb on - strand at 2026.594 kb on + strand, within RR42_RS09565 at 2026.594 kb on + strand, within RR42_RS09565 at 2026.595 kb on - strand, within RR42_RS09565 at 2027.308 kb on + strand, within RR42_RS09570 at 2027.710 kb on + strand, within RR42_RS09570 at 2027.758 kb on + strand, within RR42_RS09570 at 2027.759 kb on - strand, within RR42_RS09570 at 2027.762 kb on + strand, within RR42_RS09570 at 2028.338 kb on - strand, within RR42_RS09570 at 2028.451 kb on + strand at 2028.452 kb on - strand at 2028.452 kb on - strand at 2028.452 kb on - strand at 2028.627 kb on + strand at 2028.628 kb on - strand at 2028.628 kb on - strand at 2028.686 kb on - strand at 2028.686 kb on - strand at 2028.688 kb on - strand at 2028.688 kb on - strand at 2028.688 kb on - strand at 2028.688 kb on - strand at 2028.814 kb on + strand, within RR42_RS09575 at 2028.814 kb on + strand, within RR42_RS09575 at 2028.814 kb on + strand, within RR42_RS09575 at 2028.814 kb on + strand, within RR42_RS09575 at 2028.815 kb on - strand, within RR42_RS09575 at 2029.056 kb on + strand at 2029.057 kb on - strand at 2029.237 kb on - strand, within RR42_RS09580 at 2029.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 2,026,038 + RR42_RS09560 0.39 -1.2 2,026,039 - RR42_RS09560 0.39 -0.0 2,026,039 - RR42_RS09560 0.39 -0.5 2,026,131 - RR42_RS09560 0.54 +0.4 2,026,306 + RR42_RS09560 0.83 -0.7 2,026,307 - RR42_RS09560 0.83 +0.4 2,026,393 - -2.5 2,026,443 + +0.7 2,026,444 - -0.1 2,026,447 - +0.5 2,026,594 + RR42_RS09565 0.32 -0.8 2,026,594 + RR42_RS09565 0.32 +0.1 2,026,595 - RR42_RS09565 0.33 -0.6 2,027,308 + RR42_RS09570 0.20 -0.3 2,027,710 + RR42_RS09570 0.46 -0.6 2,027,758 + RR42_RS09570 0.49 +0.6 2,027,759 - RR42_RS09570 0.49 -1.2 2,027,762 + RR42_RS09570 0.49 +0.4 2,028,338 - RR42_RS09570 0.85 +0.7 2,028,451 + -1.0 2,028,452 - +0.4 2,028,452 - +2.0 2,028,452 - -0.2 2,028,627 + +0.3 2,028,628 - +0.3 2,028,628 - -0.8 2,028,686 - +0.1 2,028,686 - +0.8 2,028,688 - -0.8 2,028,688 - +1.2 2,028,688 - -0.5 2,028,688 - -2.5 2,028,814 + RR42_RS09575 0.15 -0.1 2,028,814 + RR42_RS09575 0.15 -0.3 2,028,814 + RR42_RS09575 0.15 +0.1 2,028,814 + RR42_RS09575 0.15 -0.1 2,028,815 - RR42_RS09575 0.15 +0.4 2,029,056 + -1.0 2,029,057 - +0.1 2,029,237 - RR42_RS09580 0.65 -1.6 2,029,460 - +1.5
Or see this region's nucleotide sequence