Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09370 and RR42_RS09375 are separated by 55 nucleotides RR42_RS09375 and RR42_RS09380 are separated by 9 nucleotides
RR42_RS09370: RR42_RS09370 - transposase, at 1,983,180 to 1,984,235
_RS09370
RR42_RS09375: RR42_RS09375 - taurine dioxygenase, at 1,984,291 to 1,985,139
_RS09375
RR42_RS09380: RR42_RS09380 - quinone oxidoreductase, at 1,985,149 to 1,986,129
_RS09380
Position (kb)
1984
1985
1986 Strain fitness (log2 ratio)
-2
-1
0
1 at 1983.602 kb on - strand, within RR42_RS09370 at 1983.830 kb on + strand, within RR42_RS09370 at 1983.830 kb on + strand, within RR42_RS09370 at 1983.831 kb on - strand, within RR42_RS09370 at 1983.831 kb on - strand, within RR42_RS09370 at 1983.836 kb on + strand, within RR42_RS09370 at 1983.837 kb on - strand, within RR42_RS09370 at 1983.837 kb on - strand, within RR42_RS09370 at 1984.085 kb on - strand, within RR42_RS09370 at 1984.085 kb on - strand, within RR42_RS09370 at 1984.169 kb on - strand at 1984.240 kb on + strand at 1984.312 kb on - strand at 1984.365 kb on - strand at 1984.365 kb on - strand at 1984.892 kb on + strand, within RR42_RS09375 at 1985.089 kb on + strand at 1985.121 kb on + strand at 1985.122 kb on - strand at 1985.151 kb on - strand at 1985.321 kb on + strand, within RR42_RS09380 at 1985.321 kb on + strand, within RR42_RS09380 at 1985.322 kb on - strand, within RR42_RS09380 at 1985.322 kb on - strand, within RR42_RS09380 at 1985.349 kb on - strand, within RR42_RS09380 at 1985.552 kb on + strand, within RR42_RS09380 at 1985.738 kb on + strand, within RR42_RS09380 at 1985.993 kb on + strand, within RR42_RS09380 at 1985.993 kb on + strand, within RR42_RS09380 at 1985.994 kb on - strand, within RR42_RS09380 at 1985.994 kb on - strand, within RR42_RS09380
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 1,983,602 - RR42_RS09370 0.40 -0.5 1,983,830 + RR42_RS09370 0.62 +0.6 1,983,830 + RR42_RS09370 0.62 +1.0 1,983,831 - RR42_RS09370 0.62 -0.5 1,983,831 - RR42_RS09370 0.62 -0.2 1,983,836 + RR42_RS09370 0.62 -0.0 1,983,837 - RR42_RS09370 0.62 -0.1 1,983,837 - RR42_RS09370 0.62 +1.4 1,984,085 - RR42_RS09370 0.86 -2.0 1,984,085 - RR42_RS09370 0.86 +0.5 1,984,169 - +0.1 1,984,240 + -0.5 1,984,312 - -0.2 1,984,365 - -0.4 1,984,365 - -2.0 1,984,892 + RR42_RS09375 0.71 -1.1 1,985,089 + +0.9 1,985,121 + +0.4 1,985,122 - -0.4 1,985,151 - +0.1 1,985,321 + RR42_RS09380 0.18 +0.5 1,985,321 + RR42_RS09380 0.18 -0.1 1,985,322 - RR42_RS09380 0.18 -0.4 1,985,322 - RR42_RS09380 0.18 -0.1 1,985,349 - RR42_RS09380 0.20 -1.2 1,985,552 + RR42_RS09380 0.41 -0.3 1,985,738 + RR42_RS09380 0.60 +1.1 1,985,993 + RR42_RS09380 0.86 -1.2 1,985,993 + RR42_RS09380 0.86 +0.7 1,985,994 - RR42_RS09380 0.86 +0.5 1,985,994 - RR42_RS09380 0.86 -0.8
Or see this region's nucleotide sequence