Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09080 and RR42_RS09085 overlap by 1 nucleotides RR42_RS09085 and RR42_RS09090 are separated by 223 nucleotides RR42_RS09090 and RR42_RS09095 are separated by 150 nucleotides
RR42_RS09080: RR42_RS09080 - C4-dicarboxylate ABC transporter permease, at 1,910,423 to 1,911,733
_RS09080
RR42_RS09085: RR42_RS09085 - C4-dicarboxylate ABC transporter permease, at 1,911,733 to 1,912,284
_RS09085
RR42_RS09090: RR42_RS09090 - C4-dicarboxylate ABC transporter substrate-binding protein, at 1,912,508 to 1,913,467
_RS09090
RR42_RS09095: RR42_RS09095 - amidase, at 1,913,618 to 1,914,961
_RS09095
Position (kb)
1912
1913
1914 Strain fitness (log2 ratio)
-2
-1
0
1 at 1911.516 kb on - strand, within RR42_RS09080 at 1911.516 kb on - strand, within RR42_RS09080 at 1911.516 kb on - strand, within RR42_RS09080 at 1911.557 kb on - strand, within RR42_RS09080 at 1912.094 kb on - strand, within RR42_RS09085 at 1912.152 kb on + strand, within RR42_RS09085 at 1912.392 kb on + strand at 1912.393 kb on - strand at 1912.413 kb on + strand at 1912.425 kb on + strand at 1912.461 kb on + strand at 1912.474 kb on - strand at 1912.519 kb on - strand at 1912.663 kb on - strand, within RR42_RS09090 at 1912.815 kb on + strand, within RR42_RS09090 at 1912.815 kb on + strand, within RR42_RS09090 at 1912.816 kb on - strand, within RR42_RS09090 at 1912.980 kb on + strand, within RR42_RS09090 at 1912.980 kb on + strand, within RR42_RS09090 at 1912.981 kb on - strand, within RR42_RS09090 at 1912.981 kb on - strand, within RR42_RS09090 at 1912.981 kb on - strand, within RR42_RS09090 at 1913.038 kb on - strand, within RR42_RS09090 at 1913.068 kb on - strand, within RR42_RS09090 at 1913.068 kb on - strand, within RR42_RS09090 at 1913.136 kb on + strand, within RR42_RS09090 at 1913.136 kb on + strand, within RR42_RS09090 at 1913.137 kb on - strand, within RR42_RS09090 at 1913.137 kb on - strand, within RR42_RS09090 at 1913.148 kb on + strand, within RR42_RS09090 at 1913.186 kb on + strand, within RR42_RS09090 at 1913.779 kb on + strand, within RR42_RS09095 at 1913.779 kb on + strand, within RR42_RS09095 at 1913.950 kb on - strand, within RR42_RS09095 at 1913.950 kb on - strand, within RR42_RS09095 at 1913.950 kb on - strand, within RR42_RS09095 at 1914.007 kb on - strand, within RR42_RS09095 at 1914.341 kb on - strand, within RR42_RS09095 at 1914.342 kb on + strand, within RR42_RS09095 at 1914.342 kb on + strand, within RR42_RS09095 at 1914.342 kb on + strand, within RR42_RS09095
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 1,911,516 - RR42_RS09080 0.83 -0.0 1,911,516 - RR42_RS09080 0.83 -1.5 1,911,516 - RR42_RS09080 0.83 -0.8 1,911,557 - RR42_RS09080 0.86 -0.6 1,912,094 - RR42_RS09085 0.65 -1.1 1,912,152 + RR42_RS09085 0.76 -0.8 1,912,392 + -0.5 1,912,393 - +0.4 1,912,413 + -1.0 1,912,425 + -0.9 1,912,461 + -0.3 1,912,474 - -0.7 1,912,519 - -0.2 1,912,663 - RR42_RS09090 0.16 +0.9 1,912,815 + RR42_RS09090 0.32 -1.1 1,912,815 + RR42_RS09090 0.32 -0.6 1,912,816 - RR42_RS09090 0.32 -0.1 1,912,980 + RR42_RS09090 0.49 +0.5 1,912,980 + RR42_RS09090 0.49 -0.8 1,912,981 - RR42_RS09090 0.49 +1.1 1,912,981 - RR42_RS09090 0.49 +0.6 1,912,981 - RR42_RS09090 0.49 -2.7 1,913,038 - RR42_RS09090 0.55 -0.4 1,913,068 - RR42_RS09090 0.58 +0.3 1,913,068 - RR42_RS09090 0.58 +0.3 1,913,136 + RR42_RS09090 0.65 -0.9 1,913,136 + RR42_RS09090 0.65 -1.0 1,913,137 - RR42_RS09090 0.66 -0.0 1,913,137 - RR42_RS09090 0.66 -1.0 1,913,148 + RR42_RS09090 0.67 -2.5 1,913,186 + RR42_RS09090 0.71 +0.3 1,913,779 + RR42_RS09095 0.12 -0.5 1,913,779 + RR42_RS09095 0.12 -0.0 1,913,950 - RR42_RS09095 0.25 -0.1 1,913,950 - RR42_RS09095 0.25 -1.1 1,913,950 - RR42_RS09095 0.25 -1.1 1,914,007 - RR42_RS09095 0.29 +0.6 1,914,341 - RR42_RS09095 0.54 -1.5 1,914,342 + RR42_RS09095 0.54 -0.2 1,914,342 + RR42_RS09095 0.54 -0.9 1,914,342 + RR42_RS09095 0.54 +0.1
Or see this region's nucleotide sequence