Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS08585 and RR42_RS08590 are separated by 14 nucleotides RR42_RS08590 and RR42_RS08595 are separated by 281 nucleotides RR42_RS08595 and RR42_RS08600 overlap by 4 nucleotides RR42_RS08600 and RR42_RS08605 overlap by 4 nucleotides RR42_RS08605 and RR42_RS08610 overlap by 4 nucleotides
RR42_RS08585: RR42_RS08585 - baseplate assembly protein, at 1,804,363 to 1,804,707
_RS08585
RR42_RS08590: RR42_RS08590 - baseplate assembly protein, at 1,804,722 to 1,805,339
_RS08590
RR42_RS08595: RR42_RS08595 - virion morphogenesis protein, at 1,805,621 to 1,806,067
_RS08595
RR42_RS08600: RR42_RS08600 - tail protein, at 1,806,064 to 1,806,504
_RS08600
RR42_RS08605: RR42_RS08605 - hypothetical protein, at 1,806,501 to 1,807,010
_RS08605
RR42_RS08610: RR42_RS08610 - peptidoglycan-binding protein, at 1,807,007 to 1,807,813
_RS08610
Position (kb) 1805
1806
1807 Strain fitness (log2 ratio) -1
0
1 at 1804.804 kb on - strand, within RR42_RS08590 at 1804.962 kb on + strand, within RR42_RS08590 at 1805.412 kb on + strand at 1805.412 kb on + strand at 1805.493 kb on + strand at 1805.494 kb on - strand at 1805.594 kb on + strand at 1805.611 kb on + strand at 1806.565 kb on - strand, within RR42_RS08605 at 1806.595 kb on + strand, within RR42_RS08605 at 1806.595 kb on + strand, within RR42_RS08605 at 1806.596 kb on - strand, within RR42_RS08605 at 1806.596 kb on - strand, within RR42_RS08605 at 1806.732 kb on - strand, within RR42_RS08605 at 1806.732 kb on - strand, within RR42_RS08605 at 1806.732 kb on - strand, within RR42_RS08605 at 1806.873 kb on + strand, within RR42_RS08605 at 1806.926 kb on - strand, within RR42_RS08605 at 1807.011 kb on - strand at 1807.044 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 1,804,804 - RR42_RS08590 0.13 +0.7 1,804,962 + RR42_RS08590 0.39 +0.2 1,805,412 + +0.7 1,805,412 + -1.0 1,805,493 + +0.6 1,805,494 - -0.5 1,805,594 + -0.0 1,805,611 + +0.3 1,806,565 - RR42_RS08605 0.13 +0.2 1,806,595 + RR42_RS08605 0.18 +0.2 1,806,595 + RR42_RS08605 0.18 +0.1 1,806,596 - RR42_RS08605 0.19 +0.3 1,806,596 - RR42_RS08605 0.19 -0.9 1,806,732 - RR42_RS08605 0.45 -0.4 1,806,732 - RR42_RS08605 0.45 +1.6 1,806,732 - RR42_RS08605 0.45 +0.0 1,806,873 + RR42_RS08605 0.73 +0.0 1,806,926 - RR42_RS08605 0.83 -0.8 1,807,011 - +0.2 1,807,044 + -0.0
Or see this region's nucleotide sequence