Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05890 and RR42_RS05895 overlap by 4 nucleotides RR42_RS05895 and RR42_RS05900 are separated by 71 nucleotides RR42_RS05900 and RR42_RS05905 are separated by 122 nucleotides RR42_RS05905 and RR42_RS05910 are separated by 93 nucleotides
RR42_RS05890: RR42_RS05890 - glutamine-synthetase adenylyltransferase, at 1,296,180 to 1,299,140
_RS05890
RR42_RS05895: RR42_RS05895 - rhombosortase, at 1,299,137 to 1,299,772
_RS05895
RR42_RS05900: RR42_RS05900 - peptidase, at 1,299,844 to 1,301,529
_RS05900
RR42_RS05905: RR42_RS05905 - hypothetical protein, at 1,301,652 to 1,302,044
_RS05905
RR42_RS05910: RR42_RS05910 - DNA repair protein, at 1,302,138 to 1,303,862
_RS05910
Position (kb)
1299
1300
1301
1302 Strain fitness (log2 ratio)
-2
-1
0
1 at 1298.859 kb on + strand at 1298.859 kb on - strand at 1298.860 kb on - strand at 1298.860 kb on - strand at 1298.860 kb on - strand at 1298.860 kb on - strand at 1298.860 kb on - strand at 1299.195 kb on + strand at 1299.229 kb on + strand, within RR42_RS05895 at 1299.229 kb on + strand, within RR42_RS05895 at 1299.263 kb on + strand, within RR42_RS05895 at 1299.721 kb on - strand at 1299.771 kb on - strand at 1300.074 kb on - strand, within RR42_RS05900 at 1300.074 kb on - strand, within RR42_RS05900 at 1300.074 kb on - strand, within RR42_RS05900 at 1300.074 kb on - strand, within RR42_RS05900 at 1300.223 kb on + strand, within RR42_RS05900 at 1300.223 kb on + strand, within RR42_RS05900 at 1300.223 kb on + strand, within RR42_RS05900 at 1300.223 kb on + strand, within RR42_RS05900 at 1300.224 kb on - strand, within RR42_RS05900 at 1300.224 kb on - strand, within RR42_RS05900 at 1300.364 kb on + strand, within RR42_RS05900 at 1300.365 kb on - strand, within RR42_RS05900 at 1300.365 kb on - strand, within RR42_RS05900 at 1300.365 kb on - strand, within RR42_RS05900 at 1300.714 kb on - strand, within RR42_RS05900 at 1300.761 kb on + strand, within RR42_RS05900 at 1300.761 kb on + strand, within RR42_RS05900 at 1300.761 kb on + strand, within RR42_RS05900 at 1300.761 kb on + strand, within RR42_RS05900 at 1300.761 kb on + strand, within RR42_RS05900 at 1300.761 kb on + strand, within RR42_RS05900 at 1300.762 kb on - strand, within RR42_RS05900 at 1300.762 kb on - strand, within RR42_RS05900 at 1300.762 kb on - strand, within RR42_RS05900 at 1300.762 kb on - strand, within RR42_RS05900 at 1300.940 kb on + strand, within RR42_RS05900 at 1301.046 kb on - strand, within RR42_RS05900 at 1301.268 kb on + strand, within RR42_RS05900 at 1301.269 kb on - strand, within RR42_RS05900 at 1301.269 kb on - strand, within RR42_RS05900 at 1301.379 kb on - strand at 1301.533 kb on + strand at 1301.533 kb on + strand at 1301.534 kb on - strand at 1301.534 kb on - strand at 1301.572 kb on + strand at 1301.925 kb on - strand at 1301.931 kb on + strand, within RR42_RS05905 at 1301.931 kb on + strand, within RR42_RS05905 at 1301.931 kb on + strand, within RR42_RS05905
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 1,298,859 + +0.2 1,298,859 - +0.1 1,298,860 - -1.8 1,298,860 - -0.7 1,298,860 - -0.7 1,298,860 - +0.2 1,298,860 - -0.1 1,299,195 + -2.0 1,299,229 + RR42_RS05895 0.14 -0.3 1,299,229 + RR42_RS05895 0.14 -0.9 1,299,263 + RR42_RS05895 0.20 +0.8 1,299,721 - -0.8 1,299,771 - -0.0 1,300,074 - RR42_RS05900 0.14 -0.4 1,300,074 - RR42_RS05900 0.14 -1.0 1,300,074 - RR42_RS05900 0.14 +0.5 1,300,074 - RR42_RS05900 0.14 -0.5 1,300,223 + RR42_RS05900 0.22 +0.2 1,300,223 + RR42_RS05900 0.22 -1.5 1,300,223 + RR42_RS05900 0.22 -2.4 1,300,223 + RR42_RS05900 0.22 -1.7 1,300,224 - RR42_RS05900 0.23 -0.4 1,300,224 - RR42_RS05900 0.23 -0.9 1,300,364 + RR42_RS05900 0.31 +0.4 1,300,365 - RR42_RS05900 0.31 +0.8 1,300,365 - RR42_RS05900 0.31 -0.8 1,300,365 - RR42_RS05900 0.31 -0.8 1,300,714 - RR42_RS05900 0.52 -0.3 1,300,761 + RR42_RS05900 0.54 -1.5 1,300,761 + RR42_RS05900 0.54 -1.3 1,300,761 + RR42_RS05900 0.54 -0.4 1,300,761 + RR42_RS05900 0.54 +0.4 1,300,761 + RR42_RS05900 0.54 -0.1 1,300,761 + RR42_RS05900 0.54 -0.2 1,300,762 - RR42_RS05900 0.54 +0.3 1,300,762 - RR42_RS05900 0.54 -1.7 1,300,762 - RR42_RS05900 0.54 +0.5 1,300,762 - RR42_RS05900 0.54 -0.5 1,300,940 + RR42_RS05900 0.65 +0.4 1,301,046 - RR42_RS05900 0.71 +0.6 1,301,268 + RR42_RS05900 0.84 -0.1 1,301,269 - RR42_RS05900 0.85 +0.2 1,301,269 - RR42_RS05900 0.85 -0.3 1,301,379 - -0.8 1,301,533 + +0.5 1,301,533 + -0.9 1,301,534 - -0.6 1,301,534 - -0.1 1,301,572 + +0.6 1,301,925 - -0.2 1,301,931 + RR42_RS05905 0.71 -1.1 1,301,931 + RR42_RS05905 0.71 +1.5 1,301,931 + RR42_RS05905 0.71 -0.5
Or see this region's nucleotide sequence