Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04340 and RR42_RS04345 are separated by 208 nucleotides RR42_RS04345 and RR42_RS04350 are separated by 243 nucleotides
RR42_RS04340: RR42_RS04340 - hypothetical protein, at 964,094 to 964,774
_RS04340
RR42_RS04345: RR42_RS04345 - iron ABC transporter permease, at 964,983 to 966,611
_RS04345
RR42_RS04350: RR42_RS04350 - ABC transporter ATP-binding protein, at 966,855 to 967,916
_RS04350
Position (kb) 964
965
966
967 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 964.075 kb on + strand at 964.096 kb on - strand at 964.275 kb on + strand, within RR42_RS04340 at 964.346 kb on + strand, within RR42_RS04340 at 964.346 kb on + strand, within RR42_RS04340 at 964.347 kb on - strand, within RR42_RS04340 at 964.401 kb on + strand, within RR42_RS04340 at 964.402 kb on - strand, within RR42_RS04340 at 964.402 kb on - strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.546 kb on + strand, within RR42_RS04340 at 964.774 kb on + strand at 964.774 kb on + strand at 964.774 kb on + strand at 964.774 kb on + strand at 964.774 kb on + strand at 964.774 kb on - strand at 964.922 kb on + strand at 964.923 kb on - strand at 964.985 kb on - strand at 965.113 kb on + strand at 965.114 kb on - strand at 965.114 kb on - strand at 965.366 kb on - strand, within RR42_RS04345 at 965.372 kb on - strand, within RR42_RS04345 at 965.512 kb on + strand, within RR42_RS04345 at 965.646 kb on - strand, within RR42_RS04345 at 965.761 kb on - strand, within RR42_RS04345 at 965.947 kb on + strand, within RR42_RS04345 at 965.980 kb on + strand, within RR42_RS04345 at 966.169 kb on + strand, within RR42_RS04345 at 966.169 kb on + strand, within RR42_RS04345 at 966.308 kb on + strand, within RR42_RS04345 at 966.311 kb on - strand, within RR42_RS04345 at 966.329 kb on - strand, within RR42_RS04345 at 966.329 kb on - strand, within RR42_RS04345 at 966.391 kb on + strand, within RR42_RS04345 at 966.391 kb on + strand, within RR42_RS04345 at 966.467 kb on + strand at 966.467 kb on + strand at 966.467 kb on + strand at 966.468 kb on - strand at 966.478 kb on + strand at 966.479 kb on - strand at 966.657 kb on + strand at 966.657 kb on + strand at 966.658 kb on - strand at 966.713 kb on - strand at 966.751 kb on - strand at 966.762 kb on + strand at 966.812 kb on + strand at 966.812 kb on + strand at 966.812 kb on + strand at 966.812 kb on + strand at 966.812 kb on + strand at 966.813 kb on - strand at 966.813 kb on - strand at 966.814 kb on + strand at 966.814 kb on + strand at 966.814 kb on + strand at 966.815 kb on - strand at 966.815 kb on - strand at 967.132 kb on - strand, within RR42_RS04350 at 967.132 kb on - strand, within RR42_RS04350 at 967.532 kb on + strand, within RR42_RS04350 at 967.532 kb on + strand, within RR42_RS04350 at 967.539 kb on + strand, within RR42_RS04350
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 964,075 + -1.4 964,096 - -0.4 964,275 + RR42_RS04340 0.27 +0.1 964,346 + RR42_RS04340 0.37 -0.4 964,346 + RR42_RS04340 0.37 -1.2 964,347 - RR42_RS04340 0.37 -0.7 964,401 + RR42_RS04340 0.45 +0.3 964,402 - RR42_RS04340 0.45 -0.3 964,402 - RR42_RS04340 0.45 -1.2 964,546 + RR42_RS04340 0.66 +0.6 964,546 + RR42_RS04340 0.66 -0.5 964,546 + RR42_RS04340 0.66 -0.7 964,546 + RR42_RS04340 0.66 +1.3 964,546 + RR42_RS04340 0.66 -0.2 964,546 + RR42_RS04340 0.66 +0.2 964,774 + +0.4 964,774 + -1.6 964,774 + -1.3 964,774 + +0.4 964,774 + -0.7 964,774 - -0.7 964,922 + -1.1 964,923 - -0.5 964,985 - -0.7 965,113 + -1.3 965,114 - -0.2 965,114 - +0.6 965,366 - RR42_RS04345 0.24 -1.2 965,372 - RR42_RS04345 0.24 -0.7 965,512 + RR42_RS04345 0.32 -0.9 965,646 - RR42_RS04345 0.41 -0.6 965,761 - RR42_RS04345 0.48 -0.5 965,947 + RR42_RS04345 0.59 -1.0 965,980 + RR42_RS04345 0.61 -2.8 966,169 + RR42_RS04345 0.73 -1.9 966,169 + RR42_RS04345 0.73 -0.4 966,308 + RR42_RS04345 0.81 -0.2 966,311 - RR42_RS04345 0.82 +0.2 966,329 - RR42_RS04345 0.83 -0.4 966,329 - RR42_RS04345 0.83 +0.4 966,391 + RR42_RS04345 0.86 -2.2 966,391 + RR42_RS04345 0.86 -1.5 966,467 + -2.3 966,467 + -2.2 966,467 + -0.5 966,468 - -0.1 966,478 + -1.0 966,479 - -0.2 966,657 + -1.0 966,657 + -0.1 966,658 - -1.4 966,713 - +0.8 966,751 - -0.4 966,762 + -0.4 966,812 + -0.9 966,812 + +0.6 966,812 + -0.0 966,812 + -1.8 966,812 + -0.3 966,813 - -0.1 966,813 - +0.1 966,814 + -0.9 966,814 + +1.2 966,814 + -0.2 966,815 - +0.3 966,815 - +1.4 967,132 - RR42_RS04350 0.26 -2.2 967,132 - RR42_RS04350 0.26 -0.6 967,532 + RR42_RS04350 0.64 +0.6 967,532 + RR42_RS04350 0.64 -0.3 967,539 + RR42_RS04350 0.64 +0.2
Or see this region's nucleotide sequence